本文選題：表面增強(qiáng)拉曼散射 + 生化監(jiān)測(cè)　； 參考：《中北大學(xué)》2017年碩士論文

【摘要】：近年來,生化恐怖襲擊活動(dòng)在全球呈蔓延趨勢(shì),食品中毒事件也是頻繁發(fā)生,而這些都是有毒有害的生化分子造成的,因此尋找準(zhǔn)確、快捷、指標(biāo)定量的有害生化分子的檢測(cè)方法意義重大。現(xiàn)有的光譜學(xué)檢測(cè)手段主要有兩種:質(zhì)譜法和光譜法。表面拉曼增強(qiáng)散射(Surface Enhanced Raman Scattering,SERS)傳感技術(shù)具有靈敏度高、選擇性好、可快速檢測(cè)的優(yōu)點(diǎn),且具有分子結(jié)構(gòu)指紋的功能,因此SERS傳感技術(shù)在光化學(xué)傳感器件領(lǐng)域展現(xiàn)出了極大的應(yīng)用潛力。傳統(tǒng)的SERS技術(shù)需要在玻璃片上單獨(dú)地制作金屬微納結(jié)構(gòu)基底,并且必須在大型拉曼光譜儀下面進(jìn)行檢測(cè),檢測(cè)設(shè)備體積大、價(jià)格昂貴,不能適應(yīng)現(xiàn)場(chǎng)檢測(cè)的需要。光纖具有低損耗、抗干擾、制作成本低、制造簡(jiǎn)單等優(yōu)點(diǎn),通過將貴金屬微/納結(jié)構(gòu)沉積在光纖的端面和表面制備的光纖SERS探針,在保持靈敏度下降不大、快速檢測(cè)特點(diǎn)的同時(shí),還能夠?qū)崿F(xiàn)對(duì)生化樣品分子的在線監(jiān)測(cè)和遠(yuǎn)程檢測(cè),因此極大地挑戰(zhàn)了傳統(tǒng)SERS技術(shù)的地位。本論文將光纖SERS探針上的光學(xué)理論與技術(shù)、現(xiàn)代MEMS/NEMS技術(shù)以及食品安全檢測(cè)迫切需求相結(jié)合,提出開展基于SERS效應(yīng)的光纖探針生化傳感技術(shù)研究,深入研究了光纖探針設(shè)計(jì)方法和制備工藝,并制備出兩種靈敏度高、穩(wěn)定性好的光纖SERS探針。利用制備的光纖SERS探針,成功實(shí)現(xiàn)了對(duì)羅丹明6G(R6G)分子的檢測(cè)。本文首先介紹了表面增強(qiáng)拉曼散射的基本概念,探討了光纖生化傳感器的基本工作原理及其應(yīng)用領(lǐng)域。之后針對(duì)傳統(tǒng)SERS技術(shù)的問題設(shè)計(jì)了一種錐柱組合型光纖SERS探針,基于光線追跡法優(yōu)化了錐柱組合型光纖SERS探針的結(jié)構(gòu)參數(shù),在以上工作基礎(chǔ)上,著重開展了對(duì)錐柱組合型光纖SERS探針制備工藝的研究,并成功實(shí)現(xiàn)了基于管腐蝕法錐柱組合型光纖SERS探針的批量制備。在此基礎(chǔ)上,論文進(jìn)一步研究了不同光纖探針錐柱區(qū)直徑和銀納米顆粒修飾時(shí)間對(duì)光纖SERS探針檢測(cè)性能的影響,建立了快速、高效、具有指紋識(shí)別檢測(cè)的錐柱組合型光纖SERS傳感器,實(shí)現(xiàn)了對(duì)R6G分子的快速痕量檢測(cè),該類型光纖SERS傳感器的檢測(cè)限達(dá)到了10-14 mol/L。論文隨后針對(duì)光纖端面SERS探針的遠(yuǎn)端檢測(cè)能力不足的問題,開展了一種基于球形端面結(jié)構(gòu)的光纖SERS探針傳感技術(shù)研究,包括球型探針結(jié)構(gòu)設(shè)計(jì)和幾何參數(shù)優(yōu)化、SERS探針表面納米顆粒表征等關(guān)鍵技術(shù)研究,開發(fā)了基于熔融法和銀溶膠自組裝法制備光纖球形SERS探針的制備工藝,成功制備出SERS探針敏感結(jié)構(gòu)。利用該SERS探針球型端面表面積大的優(yōu)點(diǎn),以R6G作為待測(cè)目的物,研究了不同直徑端面小球的SERS探針遠(yuǎn)端檢測(cè)生化分子的性能,實(shí)現(xiàn)了對(duì)R6G分子的指紋識(shí)別檢測(cè)。相比于錐柱組合型光纖SERS探針,該探針制備簡(jiǎn)單安全,穩(wěn)定性也得到了進(jìn)一步的提升,驗(yàn)證了本文提出的利用光纖SERS探針傳感器對(duì)生化目的物進(jìn)行遠(yuǎn)端在線快速痕量識(shí)別的功能。
[Abstract]:In recent years, chemical and biological terrorist attacks have spread all over the world, and food poisoning incidents have occurred frequently, and these are caused by toxic and harmful biological and chemical molecules, so it is accurate and quick to look for them.It is of great significance to detect harmful biochemical molecules with quantitative index.There are two main methods of spectroscopic detection: mass spectrometry and spectral method.Surface Enhanced Raman scattering (SERS) sensing technology has the advantages of high sensitivity, good selectivity, rapid detection, and the function of molecular structure fingerprint.Therefore, SERS sensing technology has shown great application potential in the field of photochemical sensing devices.The traditional SERS technology needs to fabricate the metal micro-nano structure substrate on the glass and must be detected under the large Raman spectrometer. The detection equipment is large and expensive and can not meet the needs of the field detection.Optical fiber has the advantages of low loss, anti-interference, low cost and simple fabrication. By depositing the noble metal micro / nano structure on the end surface and surface of fiber, the sensitivity of optical fiber SERS probe is not much decreased.At the same time, it can also realize the on-line monitoring and remote detection of biochemical sample molecules, which greatly challenges the status of traditional SERS technology.In this paper, the optical theory and technology of optical fiber SERS probe, modern MEMS/NEMS technology and the urgent need of food safety detection are combined, and the research on biochemical sensing technology of fiber optic probe based on SERS effect is put forward.The design method and fabrication process of optical fiber probe were studied in detail, and two kinds of optical fiber SERS probes with high sensitivity and good stability were prepared.The detection of Rhodamine 6GN R 6G was successfully achieved by using the optical fiber SERS probe.In this paper, the basic concept of surface-enhanced Raman scattering is introduced, and the basic working principle and application field of fiber optic biochemical sensor are discussed.Then, a cone-column composite fiber SERS probe is designed to solve the problem of traditional SERS technology. Based on the ray tracing method, the structural parameters of the cone-column composite fiber SERS probe are optimized.The fabrication process of cone-column composite fiber SERS probe was studied, and the batch fabrication of cone-column composite fiber SERS probe based on tube corrosion method was successfully realized.On this basis, the influence of the diameter of the cone column and the modification time of silver nanoparticles on the detection performance of the fiber SERS probe is further studied, and a fast and efficient method is established.The rapid trace detection of R6G molecule is achieved by using a cone-column combined fiber SERS sensor with fingerprint identification. The detection limit of this type of optical fiber SERS sensor is 10-14 mol / L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) ~ (-1).Then, aiming at the problem that the detection ability of optical fiber end SERS probe is insufficient, a kind of optical fiber SERS probe sensing technology based on spherical end structure is studied.The structure design of spherical probe and the characterization of surface nanoparticles of spherical probe were studied. The fabrication process of spherical SERS probe based on melting method and silver sol self-assembly method was developed.The sensitive structure of SERS probe was successfully prepared.Taking advantage of the large surface area of the spherical end surface of the SERS probe, using R6G as the object to be tested, the performance of the SERS probe with different diameters for detecting biochemical molecules at the far end of the probe was studied, and the fingerprint detection of the R6G molecule was realized.Compared with the cone-column composite fiber SERS probe, the preparation of the probe is simple and safe, and the stability of the probe is further improved.The proposed optical fiber SERS probe sensor for remote on-line trace recognition of biochemical targets is verified.
【學(xué)位授予單位】：中北大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TP212

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本文編號(hào)：1769102