本文選題：非酶葡萄糖傳感器　切入點(diǎn)：氧化銅　出處：《東北電力大學(xué)》2017年碩士論文

【摘要】：葡萄糖作為構(gòu)成生命體的必需化合物,快速可靠的檢測(cè)葡萄糖濃度對(duì)于當(dāng)前社會(huì)至關(guān)重要。到目前為止,葡萄糖傳感器已經(jīng)發(fā)展了四代,前三代是主體基于生物酶的葡萄糖傳感器,由于酶類傳感器對(duì)環(huán)境苛刻的要求,極大的限制了其發(fā)展,因此,第四代非酶葡萄糖傳感器應(yīng)運(yùn)而生。近年來,已有大量關(guān)于非酶葡萄糖傳感器的報(bào)道,這類傳感器主要由各種金屬與金屬氧化物及其復(fù)合材料構(gòu)建而成;其中基于過渡金屬鎳、銅的材料由于其低廉的價(jià)格與其金屬氧化物優(yōu)異的電催化性能,受到研究人員的廣泛關(guān)注。因此,本課題以鎳、銅的氧化物為研究對(duì)象,構(gòu)建非酶葡萄糖傳感器,利用XRD、SEM、EDS、AFM等表征手段對(duì)其進(jìn)行分析,進(jìn)而利用循環(huán)伏安法與電流-時(shí)間法考察其對(duì)葡萄糖檢測(cè)的電化學(xué)性能。本文主要圍繞以下幾個(gè)方面進(jìn)行研究:(1)通過溶劑熱合成法,在模板劑P123的作用下,合成納米花狀氧化鎳超薄片層,該形貌使得氧化鎳的比表面積增大,與葡萄糖分子的接觸面積變大,電化學(xué)檢測(cè)葡萄糖時(shí)可以提供更多的電子參與反應(yīng),該氧化鎳材料構(gòu)建的非酶葡萄糖傳感器靈敏度為2550.693μA·cm~(-2)·mM~(-1),并有良好的選擇性。(2)利用微波輔助法合成了氧化銅塊狀材料,并用其修飾玻碳電極構(gòu)建非酶葡萄糖傳感器,該塊狀結(jié)構(gòu)表面光滑,電化學(xué)反應(yīng)時(shí)與葡萄糖接觸面積大,具有快的反應(yīng)時(shí)間,該傳感器的靈敏度為2491.236μA·cm~(-2)·mM~(-1),有良好的選擇性,并且抗毒性優(yōu)良,但其檢測(cè)范圍比較窄。(3)利用溶劑熱合成法在模板劑P123的作用下合成表面有微小凸起的樹莓狀氧化銅材料,該形貌極大的增加了氧化銅的比表面積,增多了氧化銅的活性位點(diǎn),在電化學(xué)檢測(cè)葡萄糖時(shí)使得葡萄糖與氧化銅的接觸面積增大,反應(yīng)更加迅速,從而使得該氧化銅材料構(gòu)建的非酶葡萄糖傳感器有更高的靈敏度,為2810.01μA·cm~(-2)·mM~(-1),并對(duì)179 nM到6.5 mM寬濃度范圍的葡萄糖實(shí)現(xiàn)電化學(xué)檢測(cè),檢測(cè)下限低至59 nM(S/N=3),并具有良好的選擇性,氯離子對(duì)其的影響也可以忽略不計(jì)。綜上,本課題主要研究了氧化鎳與氧化銅作為敏感材料用于構(gòu)建非酶葡萄糖傳感器,并得出溶劑熱合成法制備的樹莓狀氧化銅材料具有最為優(yōu)良的性質(zhì),為快速、靈敏的檢測(cè)葡萄糖提供了新思路。
[Abstract]:Glucose is an essential compound that forms a living body, and rapid and reliable detection of glucose concentrations is crucial to the current society. So far, glucose sensors have been developed for four generations. The first three generations of glucose sensors are based on biological enzymes. The development of enzyme sensors is greatly limited by the demanding requirements of the environment. Therefore, the fourth generation of non-enzymatic glucose sensors have emerged as the times require in recent years. There have been numerous reports of non-enzymatic glucose sensors, which are constructed mainly of metal and metal oxides and their composite materials; based on the transition metal nickel, Due to its low price and excellent electrocatalytic performance of metal oxides, copper materials have attracted wide attention. Therefore, the non-enzymatic glucose sensor is constructed by using nickel and copper oxides as research objects. The electrochemical properties of glucose were investigated by cyclic voltammetry and current-time method. The study focused on the following aspects: 1) Solvothermal synthesis. Nanoscale nickel oxide ultrathin layer was synthesized under the action of template P123. The surface area of nickel oxide increases and the contact area with glucose molecule becomes larger. More electrons can be provided for electrochemical detection of glucose. The sensitivity of the non-enzymatic glucose sensor is 2550.693 渭 A / cm ~ (2) (m ~ (2)) ~ ((1)) the copper oxide bulk material was synthesized by microwave-assisted method, and the nonenzymatic glucose sensor was constructed by modifying the glassy carbon electrode. The bulk structure has a smooth surface, a large contact area with glucose during electrochemical reaction, and a fast reaction time. The sensor has a sensitivity of 2491.236 渭 A cm ~ (-1) ~ (-2) mm ~ (-1), good selectivity and good anti-toxicity. However, its detection range is relatively narrow. (3) the raspberry shaped copper oxide material with small protruding surface was synthesized by solvothermal synthesis under the action of template P123, which greatly increased the specific surface area of copper oxide and the active sites of copper oxide. In the electrochemical detection of glucose, the contact area between glucose and copper oxide is increased and the reaction is more rapid, which makes the non-enzymatic glucose sensor constructed by the copper oxide material more sensitive. The electrochemical detection of glucose with a wide concentration range from 179 nm to 6.5 mm was carried out. The detection limit was as low as 59nMN / N / N ~ (3 +) and had good selectivity. The effect of chloride ion on it was negligible. In this paper, nickel oxide and copper oxide were used as sensitive materials to construct non-enzymatic glucose sensors. The raspberry copper oxide materials prepared by solvothermal synthesis were characterized by their excellent properties and rapidity. Sensitive detection of glucose provides a new idea.
【學(xué)位授予單位】：東北電力大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TP212

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 孫墨杰;王世杰;;基于ZnO/石墨烯復(fù)合材料的有機(jī)磷電化學(xué)生物傳感器研究[J];東北電力大學(xué)學(xué)報(bào);2016年05期

2 趙曉慧;孫金妮;李其林;楊云慧;;基于氧化亞銅納米立方體的無酶葡萄糖傳感器的研制[J];化學(xué)傳感器;2013年03期

3 岳永海;周曉亮;王立華;韓曉東;張澤;;Cu納米線的制備及其力學(xué)性能的研究[J];電子顯微學(xué)報(bào);2011年03期

4 曹偉;宋雪梅;王波;嚴(yán)輝;;碳納米管的研究進(jìn)展[J];材料導(dǎo)報(bào);2007年S1期

相關(guān)博士學(xué)位論文 前1條

1 鐘霞;幾種復(fù)合納米材料的合成及其在葡萄糖生物傳感器中的應(yīng)用研究[D];西南大學(xué);2013年

相關(guān)碩士學(xué)位論文 前5條

1 楊璐;基于貴金屬納米線的葡萄糖無酶?jìng)鞲泻腿剂想姵匮芯縖D];湖南師范大學(xué);2014年

2 黃靜偉;基于納米線/片的非酶葡萄糖傳感器研究[D];蘭州大學(xué);2014年

3 牟瑩;電化學(xué)非酶葡萄糖傳感器的研究[D];浙江師范大學(xué);2012年

4 黃蕓;納米結(jié)構(gòu)氧化鎳的制備及其電化學(xué)性能研究[D];吉林大學(xué);2012年

5 邢亮;基于納米材料的無酶生物傳感器研究[D];湖南大學(xué);2010年

，

本文編號(hào)：1656118