本文選題：碳納米點(diǎn)　切入點(diǎn)：熒光傳感器　出處：《天津工業(yè)大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：最近,碳點(diǎn)(CDs)作為優(yōu)越的熒光團(tuán)出現(xiàn),有很多吸引人的優(yōu)點(diǎn),包括優(yōu)異的光穩(wěn)定性、尺寸小、生物相容性好、高度可調(diào)光致發(fā)光屬性、電化學(xué)發(fā)光、化學(xué)惰性、易于與分子功能化等。碳點(diǎn)作為化學(xué)傳感器,用于重金屬離子和有機(jī)物的檢測(cè)。本文的主要工作如下:1.生物質(zhì)是制備雜原子摻雜的碳納米材料的良好的前體。我們用鴿子羽毛、蛋和糞作為前體,通過(guò)熱解碳化的方法制備氮硫摻雜的熒光碳點(diǎn)(PCDs),制備的PCDs都具有較高的熒光量子產(chǎn)率分別是24.87%(PCDs-f)、17.48%(PCDs-w)、16.34%(PCDs-y)和 33.50%(PCDs-m)。我們發(fā)現(xiàn) PCDs-m對(duì)重金屬離子沒(méi)有單一的選擇性,其他的PCDs對(duì)Hg~(2+)/Fe_(3+)有較高的靈敏性和選擇性,同時(shí)PCDs-f的最低檢測(cè)限為10.3(Hg~(2+))和60.9nM(Fe_(3+))。能夠用于人臍靜脈內(nèi)皮細(xì)胞的成像,表明碳點(diǎn)的低毒性和較好的生物相容性。2.氮和硼摻雜的碳點(diǎn)(BCNDs1-3)的制備是從三種不同的硼酸通過(guò)水熱法合成的。BCNDs擁有良好的水溶性和較高的熒光量子產(chǎn)率分別為29.01%、51.42%和68.28%。隨后,BCNDs作為H2+和三硝基苯酚(TNP)良好的的探針。有效的選擇性檢測(cè)Hf+可以歸因于通過(guò)有效的電子轉(zhuǎn)移過(guò)程促進(jìn)BCNDs無(wú)輻射電子/空穴復(fù)合湮滅和TNP的檢測(cè)可以歸因于熒光共振能量轉(zhuǎn)移過(guò)程。結(jié)果表明BCNDs2對(duì)Hg~(2+)的和TNP的檢測(cè)是最靈敏的熒光探針。3.碳點(diǎn)與4-羧基苯硼酸(CPBA)共價(jià)結(jié)合,合成4-羧基苯硼酸功能化的碳點(diǎn)(CPBA-CDs)。CPBA-CDs能與鄰苯二酚的鄰二醇結(jié)構(gòu)共價(jià)結(jié)合,主要是通過(guò)表面淬火誘導(dǎo)機(jī)制,從而使CPBA-CDs的熒光猝滅。檢測(cè)鄰苯二酚的線性范圍為0.1-56 μM檢測(cè)限為115 nM。根據(jù)CPBA-CDs熒光發(fā)射光譜可以實(shí)現(xiàn)在細(xì)胞里多色成像。4.溴乙酰溴功能化的碳點(diǎn)(CDs-Br)用于直接檢測(cè)谷胱甘肽(GSH)。溴乙酰溴功能化的部分(鹵素部分)能夠使CDs-Br的熒光發(fā)生猝滅,然而這個(gè)鹵素部分可以被巰基取代,從而是CDs-Br的熒光恢復(fù)。在優(yōu)化的條件下,探針CDs-Br 對(duì)谷胱甘肽有良好的選擇性,超過(guò)了半胱氨酸和高半胱氨酸。同時(shí)可以用于細(xì)胞里面檢測(cè)谷胱甘肽和細(xì)胞成像。
[Abstract]:Recently, carbon dots have emerged as superior fluorescent groups with many attractive advantages, including excellent photostability, small size, good biocompatibility, highly tunable photoluminescence properties, electrochemiluminescence, chemical inertia, Carbon spots are used as chemical sensors for the detection of heavy metal ions and organic compounds. The main work of this paper is as follows: 1.Biomass is a good precursor for the preparation of hetero-atom-doped carbon nanomaterials. We use pigeon feathers, Egg and feces were used as precursors to prepare nitrogen-sulfur doped fluorocarbon dots by pyrolytic carbonization. The PCDs obtained by pyrolysis and carbonation had high fluorescence quantum yields of 24.87% and 17.48% respectively. We found that PCDs-m had no single selectivity for heavy metal ions. The other PCDs have high sensitivity and selectivity for Hg~(2 / FeS-1), and the minimum detection limit for PCDs-f is 10.3hgf2) and 60.9nM / FeS-1, which can be used in the imaging of human umbilical vein endothelial cells (HUVECs). The results show that the low toxicity and good biocompatibility of carbon dots. 2. The preparation of nitrogen and boron doped carbon dots BCNDs1-3). BCNDs synthesized by hydrothermal method from three different boric acids have good water solubility and high fluorescence quantum yield of 29.01%. Then BCNDs were good probes for H2 and trinitrophenol. The effective selective detection of HF can be attributed to the combination of radiation free electron / hole annihilation and TNP detection through effective electron transfer process. Because of the fluorescence resonance energy transfer process, the results show that the detection of Hg~(2 by BCNDs2 and TNP is the most sensitive fluorescence probe .3.The carbon point is covalently bound to 4-carboxyphenylborate. The synthesis of 4-carboxyphenylboric acid functionalized carbon dot CPBA-CDsN. CPBA-CDs can bind covalently with the structure of catechol, mainly through the mechanism of surface quenching induction. The linear range of detection of catechol is from 0.1 to 56 渭 M. according to the fluorescence emission spectrum of CPBA-CDs, it can be realized in the cell of polychromatic imaging .4. the bromoacetyl bromide functionalized carbon dot CDs-Br) can be used to detect the valley directly. The functionalized part of bromoacetyl bromide (halogen part) can quench the fluorescence of CDs-Br. However, this halogen can be replaced by sulfhydryl, which is the fluorescence recovery of CDs-Br. Under optimized conditions, the probe CDs-Br has good selectivity for glutathione. More than cysteine and homocysteine. Can also be used in cell detection of glutathione and cell imaging.
【學(xué)位授予單位】：天津工業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TP212

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