本文關(guān)鍵詞：反射式光纖SPR生物傳感器的研究及測試實驗　出處：《山東大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 反射式 傾斜光纖光柵 表面等離子體共振 生物傳感器

【摘要】：光纖表面等離子體共振(SPR)技術(shù)因其免標(biāo)記、實時檢測、靈敏度高等特點被應(yīng)用到生物檢測及環(huán)境監(jiān)測等領(lǐng)域,傾斜光纖光柵(TFBG)由于光柵與纖芯軸向存在一定夾角的特殊結(jié)構(gòu)會產(chǎn)生反向傳輸?shù)陌鼘幽？梢约ぐl(fā)表面等離子體共振,TFBG與SPR技術(shù)相結(jié)合在生物傳感領(lǐng)域得到廣泛應(yīng)用。本文設(shè)計了一種反射式TFBG-SPR生物傳感器,既具有性能穩(wěn)定、耐腐蝕、免標(biāo)記等特點,還具有制作工藝簡單、實現(xiàn)二次耦合及現(xiàn)場檢測等優(yōu)勢。首先對SPR光纖傳感器、TFBG-SPR傳感器及反射式光纖傳感器的研究現(xiàn)狀進(jìn)行了總結(jié),介紹了反射式SPR傳感器的理論基礎(chǔ),分析了反射式TFBG-SPR傳感器的基本原理及傳感特性,為傳感器的制作提供理論基礎(chǔ)。其次,對反射式TFBG-SPR光纖傳感器進(jìn)行結(jié)構(gòu)設(shè)計,選擇合適的制作工藝完成反射式TFBG-SPR傳感器的制備,并對制作完成的傳感器進(jìn)行了氯化鎂溶液實驗,檢測傳感器的折射率敏感度為564.1nm/RIU,比普通TFBG的靈敏度提高了 245倍,并將該傳感器應(yīng)用到細(xì)菌濃度檢測實驗中,搭建實驗平臺并實時監(jiān)測細(xì)菌濃度的變化,為后續(xù)的細(xì)菌測試及細(xì)胞實驗奠定了基礎(chǔ)。最后,將反射式TFBG-SPR傳感器應(yīng)用到兩種生物分子實驗研究中,分別為DNA分子實驗和蛋白質(zhì)分子實驗。DNA分子選取的是耐甲氧西林金黃色葡萄球菌(MRSA)一段耐藥基因,將固定探針增覆在傳感器的金膜表面制作成反射式TFBG-SPR的DNA分子探針,再與互補(bǔ)探針發(fā)生特異性結(jié)合反應(yīng),通過觀察傳感器反射譜諧振峰對應(yīng)的共振波長偏移情況來實現(xiàn)對反應(yīng)是否進(jìn)行及反應(yīng)速度的判斷和分析。反射式TFBG-SPR蛋白質(zhì)分子實驗選取半胱胺鹽酸鹽作為將抗原固定在金膜表面的中間物質(zhì),實現(xiàn)兔IgG分子的固定,制備成蛋白質(zhì)分子探針,進(jìn)行雜交實驗,對相應(yīng)抗體山羊抗兔IgG實現(xiàn)特異性識別,雜交實驗過程中反射譜的共振波長共偏移了 9.5nm,實驗效果良好,驗證了兩種生物分子實驗的可行性。
[Abstract]:Optical fiber surface plasmon resonance (SPR) technology has been applied to biological detection and environmental monitoring because of its characteristics of non-marking, real-time detection and high sensitivity. Tilted fiber grating (TFBG) can excite the surface plasmon resonance (SPR) because of the special structure with a certain angle between the grating and the core. The combination of TFBG and SPR technology has been widely used in the field of biosensors. In this paper, a reflective TFBG-SPR biosensor has been designed, which has the characteristics of stable performance, corrosion resistance, no marking and so on. It also has the advantages of simple fabrication process, secondary coupling and field detection. Firstly, the research status of TFBG-SPR sensor and reflective fiber optic sensor are summarized. This paper introduces the theoretical basis of reflective SPR sensor, analyzes the basic principle and sensing characteristics of reflective TFBG-SPR sensor, and provides a theoretical basis for the fabrication of the sensor. Secondly. The structure of reflective TFBG-SPR optical fiber sensor is designed and the appropriate fabrication process is selected to complete the fabrication of reflective TFBG-SPR sensor. The results show that the sensitivity of the sensor is 564.1 nm / r RIUs, which is 245-fold higher than that of conventional TFBG. The sensor is applied to the bacterial concentration detection experiment. The platform is built and the change of bacterial concentration is monitored in real time, which lays a foundation for the subsequent bacterial testing and cell experiment. Finally. The reflective TFBG-SPR sensor is applied to two kinds of biomolecular experiments. DNA molecular test and protein molecular experiment. The drug resistant gene of methicillin-resistant staphylococcus aureus was selected. The fixed probe was added to the gold film surface of the sensor to form a reflective TFBG-SPR DNA molecular probe and then reacted with the complementary probe specifically. By observing the resonance wavelength offset corresponding to the resonance peak of the reflectance spectrum of the sensor, the determination and analysis of whether or not the reaction was carried out and the reaction rate were realized. Cysteamine salt was selected in the molecular experiment of reflective TFBG-SPR protein. The acid salt is the intermediate substance in which the antigen is immobilized on the surface of the gold film. The rabbit IgG molecule was immobilized, protein molecular probe was prepared, hybridization experiment was carried out, and the specific recognition of goat anti-rabbit IgG was realized. The resonance wavelength of the reflectance spectrum deviated 9.5 nm in the hybridization experiment, which proved the feasibility of the two kinds of biomolecular experiments.
【學(xué)位授予單位】：山東大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：TP212.3

【參考文獻(xiàn)】

相關(guān)期刊論文 前8條

1 艾瑞波;劉超;孫祺;牟海維;;傾斜光纖光柵透射光譜對外界介質(zhì)折射率的響應(yīng)規(guī)律研究[J];科學(xué)技術(shù)與工程;2013年11期

2 高錦航;劉瑞;童歡;李獻(xiàn);強(qiáng)鷗;唐承薇;;表面等離子共振技術(shù)測定生長抑素含量[J];高等學(xué)�；瘜W(xué)學(xué)報;2010年07期

3 張曉麗;梁大開;曾捷;趙志遠(yuǎn);曾建民;;基于光纖SPR光譜分析的污水降解過程監(jiān)測研究[J];光譜學(xué)與光譜分析;2010年02期

4 徐常龍;曹小華;柳閩生;喻國貞;;自組裝單層膜的研究[J];江西師范大學(xué)學(xué)報(自然科學(xué)版);2009年02期

5 曾捷;梁大開;曾振武;杜艷;;反射式光纖表面等離子體波共振傳感器特性研究[J];光學(xué)學(xué)報;2007年03期

6 陳莉莉,陳靜,杜毅,沈旭,蔣華良;基于生物傳感器BiacoreS51技術(shù)的新藥發(fā)現(xiàn)與開發(fā)[J];生命的化學(xué);2004年06期

7 宋磊,劉海濱,任新;反射式光強(qiáng)調(diào)制型光纖壓力傳感器[J];儀表技術(shù)與傳感器;1994年03期

8 安福生;;強(qiáng)度型光纖傳感器反射表面ρ值的調(diào)制效應(yīng)[J];光學(xué)儀器;1986年06期

相關(guān)博士學(xué)位論文 前1條

1 蔡浩原;一種新型表面等離子體諧振（SPR）生化分析系統(tǒng)的研究[D];中國科學(xué)院研究生院（電子學(xué)研究所）;2003年

相關(guān)碩士學(xué)位論文 前2條

1 朱芮;終端反射式光纖表面等離子共振傳感器的研究及其應(yīng)用[D];天津大學(xué);2012年

2 袁克皋;表面等離子共振檢測技術(shù)及其傳感器實驗裝置研究[D];浙江大學(xué);2006年

，

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