修尾蚜是一種危害廣泛的農(nóng)作物害蟲,昆蟲病原真菌蠟蚧輪枝菌（V.lecanii）和球孢白僵菌（B.bassiana）是一種對環(huán)境友好、靶標特異、能夠有效防治多種刺吸式昆蟲的生物農(nóng)藥。本研究測定了兩株球孢白僵菌（B76和B252）和兩株蠟蚧輪枝菌（L2和L5）對豆蚜的防治作用。用B76、B252、L2和L5及其二元組合（L2+B76、L2+B525、B76+L5和L2+B76+B252+L5）的濾液和分生孢子制劑進行了三次生物測定。在濾液和分生孢子的比較生物測定中,每個菌株的真菌濾液和分生孢子均為2ml。采用三種不同濃度(即1×10⁶、1×10⁷和1×10⁸ conidia mL^-1)進行二元組合生物測定。結果表明,在所有處理的第8天,對供試蚜蟲的致病力最大。在孢子最高濃度為1×10⁸ conidia mL^-1的處理下,B76菌株（85.3%）和L2菌株（77.9%）的蚜蟲死亡率均高于B252（66.0%）和L5（60.9%）。同樣,B76和L2菌株的二元組合... 

【文章來源】：中國農(nóng)業(yè)科學院北京市

【文章頁數(shù)】：125 頁

【學位級別】：博士

【文章目錄】：
附件
摘要
ABSTRACT
Abbreviation
CHAPTER Ⅰ INTRODUCTION AND REVIEW OF LITERATURE
    1.1 APHIDS
        1.1.1 Control aphids by chemical pesticides
        1.1.2 Control aphids by biological pesticides
    1.2 INTERACTION OF APHID AND PLANT
    1.3 BIOLOGICAL CONTROL
        1.3.1 Type of biological control
        1.3.2 Type of biological control agents
        1.3.3 Advantages and disadvantages of biological control
    1.4 POTENTIAL OF ENTOMOPATHOGENIC FUNGI
        1.4.1 Entomopathogenic potential of Verticillium lecanii
        1.4.2 Entomopathogenic potential of Beauveria bassiana
    1.5 FUNGAL PROTEINS OR ENZYMES
        1.5.1 Chitinases
        1.5.2 Lipases
    1.6 PLANT DEFENCE AGAINST INSECT
    1.7 DEFENCE ELICITORS OF PLANT
    1.8 BIOTIC AND ABIOTIC RELATED STRESSES PATHWAY
        1.8.1 Salicylic acid signaling pathway
        1.8.2 Jasmonic acid signaling pathway
    1.9 OBJECTIVES
CHAPTER Ⅱ BIOCONTROL POTENTIAL OF SOME ENTOMOPATHOGENIC FUNGAL STRAINS AGAINST BEAN APHID MEGOURA JAPONICA MATSUMURA
    2.1 INTRODUCTION
    2.2 MATERIALS AND METHODS
        2.2.1 Insect culture
        2.2.2 Fungal isolates
        2.2.3 Conidial suspension
        2.2.4 Fungal filtrate
        2.2.5 Pathogenicity bioassays
        2.2.6 Data analysis
    2.3 RESULTS
        2.3.1 Inhibition effect of different concentrations of the fungal spore on bean aphid
        2.3.2 Fungal strain combinations against bean aphid
        2.3.3 Comparison of the mortality of bean aphid by fungi filtrate and conidia
CHAPTER Ⅲ IDENTIFICATION,PURIFICATION AND CHARACTERIZATION OF HR INDUCING PROTEIN IN TOBACCO
    3.1 INTRODUCTION
    3.2 MATERIALS AND METHODS
        3.2.1 Fungal strains
        3.2.2 Protein elicitor purification
        3.2.3 Gene identification
        3.2.4 Gene amplification and cloning
        3.2.5 Expression and purification of the recombinant protein elicitor
        3.2.6 Characterization of the Pev L1 protein elicitor
    3.3 RESULTS
        3.3.1 Purification of protein elicitor
        3.3.2 Protein Pev L1 induced hypersensitive response HR in tobacco leaves
        3.3.3 Gene amplification and cloning
        3.3.4 Expression and purification of recombinant protein elicitor Pev L1
CHAPTER Ⅳ A NOVEL PROTEIN ELICITOR PEVL1,FROM VERTICILLIUM LECANII STRAIN L2,INDUCES SYSTEMIC RESISTANCE AGAINST BEAN APHID（MEGOURA JAPONICA MATSUMURA）IN PHASEOLUS VULGARIS L
    4.1 INTRODUCTION
    4.2 MATERIALS AND METHODS
        4.2.1 Plant cultures and insect rearing
        4.2.2 Aphid choice test
        4.2.3 Effects of the Pev L1 protein on the reproductive biology of bean aphid
        4.2.4 Bioassays with Elicitor Protein Pev L1 for checking gene expression
        4.2.5 Plant RNA isolation and c DNA synthesis
        4.2.6 Reverse transcription-quantitative PCR（RT-q PCR）
        4.2.7 Experiment design and data analyses
    4.3 RESULTS
        4.3.1 Aphid choice test
        4.3.2 Effects of the Pev L1 protein on the reproductive biology of bean aphid
        4.3.3 Expression levels of SA-pathway genes in P.vulgaris L.
        4.3.4 Expression levels of JA-pathway genes in P.vulgaris L.
CHAPTER Ⅴ DISCUSSION AND SUMMARY
    5.1 DISCUSSION
    5.2 SUMMARY
CONCLUSION
REFERENCES
ACKNOWLEDGEMENTS
RESUME



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