舞毒蛾高毒力Bt殺蟲蛋白及協(xié)同增效組合的篩選
發(fā)布時間：2019-07-03 17:14
【摘要】：舞毒蛾Lymantria dispar(Linnaeus)是世界性的重要農林業(yè)害蟲。研究Bt蛋白對舞毒蛾幼蟲的毒殺作用,篩選出高毒力Bt蛋白及具有協(xié)同增效作用的蛋白組合,將為舞毒蛾的生物防治提供依據。本研究提取了十一種Bt蛋白對三日齡舞毒蛾幼蟲進行初步篩選,初篩結果顯示Cry 1Ac、Cry 2Ab、Cry 1Ca、Vip3Aj四種蛋白具有較高毒力。進而分別測定了這四種蛋白7天的LC50。結果表明,殺蟲毒力最高的為Cry1Ac,其LC50達到了2.47 μg/g;Cry3Ab和Cry1Ca的LC50分別為29.19μg/g和42.45μg/g,表現(xiàn)出了較強的殺蟲活性；而Vip3Aj的LC50為101.26 μg/g,具有中等毒力,該結果是對Vip3Aj具有殺蟲活性的首次報道。進一步將4種蛋白組合(Crr1Ac與Cry2Ab、Cry1Ac與Cry1Ca、Cry1Ac與Vip3Aj、 Cry2Ab與Cry1Ca)按1：1混配,測定蛋白組合對舞毒蛾的殺蟲活性和增效作用。結果發(fā)現(xiàn),Cry1Ac+Cry2Ab混配蛋白LC50達到0.64 μg/g,協(xié)同毒力指數最高,為7.098,具有很強的協(xié)同增效作用；Cry1Ac+Cry1Ca和Cry1Ac+Vip3Aj混配蛋白LC50分別達到0.76μg/g和1.06μg/g,協(xié)同毒力指數分別為6.162和4.539,具有顯著的增效作用。
[Abstract]:Lymantria dispar (Linnaeus) is an important agricultural and forestry pest in the world. To study the toxic effect of Bt protein on the larvae of Plutella xylostella, and to screen out the high virulence Bt protein and the protein combination with synergistic effect, which will provide the basis for the biological control of Plutella xylostella. In this study, 11 kinds of Bt proteins were extracted to screen the larvae of three-day-old Plutella xylostella. The results showed that Cry 1AC, Cry 2Ab, Cry 1Caand Vip3Aj proteins were highly virulent. Furthermore, the LC50. of these four proteins were determined for 7 days. The results showed that the highest insecticidal toxicity was that the LC50 of Cry1Ac, reached 2.47 渭 g 路g / g and the LC50 of Cry1Ca was 29.19 渭 g / g and 42.45 渭 g / g, respectively, showing strong insecticidal activity, while the LC50 of Vip3Aj was 101.26 渭 g 路g / g, which was the first report of insecticidal activity against Vip3Aj. Furthermore, the insecticidal activity and synergistic effect of four protein combinations (Crr1Ac and Cry2Ab,Cry1Ac and Cry1Ca,Cry1Ac and Vip3Aj, Cry2Ab and Cry1Ca) on Plutella xylostella were determined at 1:1. The results showed that the LC50 of Cry1Ac Cry2Ab mixed protein reached 0.64 渭 g / g, and the synergistic toxicity index was the highest, 7.098, which had a strong synergistic effect, and the LC50 and Cry1Ac Vip3Aj mixed protein LC50 reached 0.76 渭 g / g and 1.06 渭 g / g, respectively, and the synergistic toxicity index was 6.162 and 4.539, respectively.
【學位授予單位】：北京林業(yè)大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S433.4
[Abstract]:Lymantria dispar (Linnaeus) is an important agricultural and forestry pest in the world. To study the toxic effect of Bt protein on the larvae of Plutella xylostella, and to screen out the high virulence Bt protein and the protein combination with synergistic effect, which will provide the basis for the biological control of Plutella xylostella. In this study, 11 kinds of Bt proteins were extracted to screen the larvae of three-day-old Plutella xylostella. The results showed that Cry 1AC, Cry 2Ab, Cry 1Caand Vip3Aj proteins were highly virulent. Furthermore, the LC50. of these four proteins were determined for 7 days. The results showed that the highest insecticidal toxicity was that the LC50 of Cry1Ac, reached 2.47 渭 g 路g / g and the LC50 of Cry1Ca was 29.19 渭 g / g and 42.45 渭 g / g, respectively, showing strong insecticidal activity, while the LC50 of Vip3Aj was 101.26 渭 g 路g / g, which was the first report of insecticidal activity against Vip3Aj. Furthermore, the insecticidal activity and synergistic effect of four protein combinations (Crr1Ac and Cry2Ab,Cry1Ac and Cry1Ca,Cry1Ac and Vip3Aj, Cry2Ab and Cry1Ca) on Plutella xylostella were determined at 1:1. The results showed that the LC50 of Cry1Ac Cry2Ab mixed protein reached 0.64 渭 g / g, and the synergistic toxicity index was the highest, 7.098, which had a strong synergistic effect, and the LC50 and Cry1Ac Vip3Aj mixed protein LC50 reached 0.76 渭 g / g and 1.06 渭 g / g, respectively, and the synergistic toxicity index was 6.162 and 4.539, respectively.
【學位授予單位】：北京林業(yè)大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S433.4
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