【摘要】：黑曲霉(Aspergillus niger)YF1菌株是本實(shí)驗(yàn)室篩選得到的對(duì)煙嘧磺隆具有較強(qiáng)降解能力的菌株。為優(yōu)化YF1菌株的降解條件,本試驗(yàn)分別對(duì)葡萄糖濃度、培養(yǎng)溫度、初始pH和轉(zhuǎn)速進(jìn)行單因素試驗(yàn),結(jié)果表明,YF1菌株降解煙嘧磺隆的最適條件為:葡萄糖濃度5g/L、培養(yǎng)溫度30℃、初始pH值6.50和轉(zhuǎn)速50r/min。本試驗(yàn)在研究其降解條件的同時(shí),發(fā)現(xiàn)溶液的pH均出現(xiàn)下降,隨后采用高效液相色譜檢測(cè)草酸、L-蘋果酸、α-酮戊二酸、乙酸、檸檬酸、D-蘋果酸、琥珀酸的標(biāo)準(zhǔn)品,通過(guò)保留時(shí)間確定樣品中的有機(jī)酸為草酸,但未檢測(cè)出其它6種有機(jī)酸,因此,可以確定YF1菌株可以代謝葡萄糖產(chǎn)生草酸。本試驗(yàn)初步明確了黑曲霉YF1菌株對(duì)煙嘧磺隆的降解特性,為該菌株的應(yīng)用奠定了基礎(chǔ)。
[Abstract]:Aspergillus Niger (Aspergillus niger) YF1 is a strain screened in our laboratory which has strong degradation ability to nicosulfuron-methyl. In order to optimize the degradation conditions of YF1 strain, glucose concentration, culture temperature, initial pH and rotation speed were tested by single factor test. The results showed that the optimum conditions for degradation of nicosulfuron-methyl by YF1 strain were glucose concentration of 5 g / L, and rotation rate of 5 g / L, respectively. The culture temperature was 30 鈩，

本文編號(hào)：2461347