【摘要】：為更好地將保水劑應(yīng)用到干旱山地的容器苗造林中,以丙烯酰胺-丙烯酸鉀交聯(lián)共聚物型保水劑作為供試材料,設(shè)置育苗時(NS)和造林時(AS)各加4.0 g保水劑的兩種保水劑應(yīng)用方式,利用傳統(tǒng)的平板計數(shù)法和現(xiàn)代的末端限制性片段長度多態(tài)性分析(Terminal restricti on fragment length polymorphism,T-RFLP)技術(shù),研究NS和AS對側(cè)柏容器苗造林一年后根際土壤微生物種群結(jié)構(gòu)及對側(cè)柏干旱適應(yīng)能力的影響.結(jié)果表明:保水劑可顯著提高側(cè)柏根際土壤細(xì)菌、真菌的數(shù)量和微生物量碳、氮的含量,且NS處理根際土壤的細(xì)菌數(shù)量和微生物量碳分別比AS處理提高21.65%和8.04%;保水劑及其應(yīng)用方式對造林初期側(cè)柏根際土壤的細(xì)菌群落結(jié)構(gòu)產(chǎn)生較大影響,保水劑顯著增加了根際土壤中細(xì)菌群落結(jié)構(gòu)的多樣性,NS處理具有最高的豐富度指數(shù)、多樣性指數(shù)和最低的優(yōu)勢度指數(shù);此外,同對照相比,NS處理還顯著提高了側(cè)柏容器苗的造林成活率、葉片的凈光合速率和相對含水量,降低了相對電導(dǎo)率;同AS處理相比,NS處理的造林成活率和葉片相對含水量分別提高了11.64%和8.33%,而相對電導(dǎo)率降低(降低12.19%).綜上說明,保水劑通過改善側(cè)柏容器苗根際土壤的微生物種群結(jié)構(gòu)而提高植物的干旱適應(yīng)能力,利用添加保水劑的容器苗造林具有更好的應(yīng)用效果.
[Abstract]:In order to better apply water-retaining agent to container plantlet afforestation in arid mountain area, acrylamide potassium acrylate crosslinked copolymer type water-retaining agent was used as test material. (Terminal restricti on fragment length polymorphism, was analyzed by using traditional plate counting method and modern terminal restriction fragment length polymorphism (TRFLP) by setting up two water retaining agents, (NS) during seedling raising and (AS) with 4.0g water retaining agent respectively during silviculture, and by means of traditional plate counting method and modern terminal restriction fragment length polymorphism (TRFLP). The effects of NS and AS on the soil microbial population structure and drought adaptability of Platycladus orientalis were studied by T-RFLP technique. The results showed that the amount of bacteria, fungi, microbial biomass carbon and nitrogen in rhizosphere soil of Platycladus orientalis could be increased by 21.65% and 8.04%, respectively, compared with that of AS treatment, and the amount of bacteria and microbial biomass carbon in rhizosphere soil treated with NS were increased by 21.65% and 8.04%, respectively. The microbial community structure of rhizosphere soil of Platycladus orientalis was greatly affected by water retaining agent and its application methods, and the diversity of bacterial community structure in rhizosphere soil was significantly increased by water retaining agent. NS treatment had the highest richness index. Diversity index and lowest dominance index; In addition, compared with the control, NS treatment also significantly increased the survival rate of plantlets, the net photosynthetic rate and relative water content of leaves, and decreased the relative electrical conductivity. Compared with AS treatment, the survival rate and leaf relative water content of NS treatment increased by 11.64% and 8.33%, respectively, while the relative conductivity decreased (12.19%). It is concluded that the water-retaining agent can improve the drought adaptability of the plants by improving the microbial population structure of the rhizosphere soil of the container seedlings of Platycladus orientalis.
【作者單位】： 山東省林業(yè)科學(xué)研究院山東省森林植被生態(tài)修復(fù)工程技術(shù)研究中心;德州學(xué)院;
【基金】：山東省科技發(fā)展計劃項目(2010GSF10621) 山東省農(nóng)業(yè)重大應(yīng)用技術(shù)創(chuàng)新項目資助~~
【分類號】：S791.38;S714.3
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本文編號：2387440