施氮肥對華北平原土壤氨氧化細(xì)菌和古菌數(shù)量及群落結(jié)構(gòu)的影響
發(fā)布時間：2018-12-19 20:50
【摘要】：利用熒光定量PCR、末端限制性片段長度多樣性(T-RFLP)和基因克隆文庫技術(shù),比較了4種施氮水平(不施氮肥,0 kg N/hm~2,CK;施低水平氮肥,75 kg N/hm~2,N1;施中水平氮肥,150 kg N/hm~2,N2;施高水平氮肥,225 kg N/hm~2,N3)下華北平原地區(qū)小麥季表層(0—20 cm)土壤總細(xì)菌、氨氧化細(xì)菌(AOB)和氨氧化古菌(AOA)的豐度和群落結(jié)構(gòu)。結(jié)果表明,土壤總細(xì)菌、AOB和AOA數(shù)量分別在每克干土5.74×10~9—7.50×10~9、8.89×10~6—2.66×10~7和3.83×10~8—7.78×10~8之間。不同施氮量土壤AOA數(shù)量均高于AOB數(shù)量,AOA/AOB值在81.72—14.38之間。增施氮肥顯著顯著提高AOB數(shù)量(P0.05),對總細(xì)菌和AOA數(shù)量的影響不顯著(P0.05)。與CK相比,處理N1、N2和N3中AOB數(shù)量分別提高了0.64、1.50和1.99倍。增施氮肥顯著改變了AOB和AOA的群落結(jié)構(gòu),且不同施氮量處理中AOB群落結(jié)構(gòu)差異更大。系統(tǒng)進(jìn)化分析顯示,施氮肥小麥土壤AOB主要為Nitrosospira屬類群,分布在Cluster 3的兩個分支中;AOA分布在Cluster S的4個分支中。相關(guān)性分析顯示,AOB數(shù)量與全氮和銨態(tài)氮含量呈顯著正相關(guān)關(guān)系,與土壤pH和碳氮比呈顯著負(fù)相關(guān)關(guān)系(P0.05);AOA數(shù)量與硝態(tài)氮含量和土壤pH呈顯著正相關(guān)關(guān)系,與銨態(tài)氮含量呈顯著負(fù)相關(guān)關(guān)系(P0.05)。研究結(jié)果表明:增施氮肥可顯著改變?nèi)A北平原地區(qū)堿性土壤AOB數(shù)量與群落結(jié)構(gòu),該地區(qū)小麥土壤中AOB比AOA對氮肥響應(yīng)更敏感。
[Abstract]:Using fluorescent quantitative PCR, terminal restriction fragment length diversity (T-RFLP) and gene cloning library technique, we compared four nitrogen levels (0 kg N / hmm2 CK, 75 kg N / hmm2 N 1) and 75 kg N / hm2 N 1, respectively. Applying medium level nitrogen fertilizer, 150 kg N / hm ~ (2) N _ (2); The abundance and community structure of soil total bacteria, ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing palaeomycetes (AOA) in wheat season surface layer (0-20 cm) in North China Plain were studied by applying high level nitrogen fertilizer, 225 kg N / hmm-2 N 3). The results showed that the total number of bacteria, AOB and AOA in soil ranged from 5.74 脳 10 ~ (-9) to 7.50 脳 10 ~ (10) ~ 9 ~ (9) C ~ (-1) 8.89 脳 10 ~ (6) ~ 2.66 脳 10 ~ (7) and 3.83 脳 10 ~ (8) 脳 10 ~ (8) 脳 10 ~ (8), respectively. The amount of AOA was higher than that of AOB, and the AOA/AOB value was between 81.72-14.38. Increasing nitrogen fertilizer significantly increased the number of AOB (P0.05), but had no significant effect on total bacteria and AOA (P0.05). Compared with CK, the number of AOB in N _ 1N _ 2 and N _ 3 was increased by 0.64% and 1.99 times, respectively. The community structure of AOB and AOA was significantly changed by nitrogen fertilizer application, and the difference of AOB community structure was greater in different nitrogen application treatments. Phylogenetic analysis showed that the soil AOB of nitrogen fertilized wheat was mainly Nitrosospira group, distributed in two branches of Cluster 3, and AOA was distributed in four branches of Cluster S. Correlation analysis showed that the amount of AOB was positively correlated with total nitrogen and ammonium nitrogen content, and negatively correlated with soil pH and C / N ratio (P0.05). There was a significant positive correlation between the amount of AOA and nitrate nitrogen content and soil pH content, and a significant negative correlation between the amount of AOA and ammonium nitrogen content (P0.05). The results showed that the amount of AOB and community structure of alkaline soil in North China Plain could be significantly changed by increasing nitrogen fertilizer. AOB in wheat soil was more sensitive to nitrogen fertilizer than AOA.
【作者單位】： 中國農(nóng)業(yè)大學(xué)農(nóng)學(xué)院;
【基金】：公益性行業(yè)(農(nóng)業(yè))科研專項(201503121-11) 河北省科技攻關(guān)項目(14227008D)
【分類號】：S154.3
本文編號：2387434
[Abstract]:Using fluorescent quantitative PCR, terminal restriction fragment length diversity (T-RFLP) and gene cloning library technique, we compared four nitrogen levels (0 kg N / hmm2 CK, 75 kg N / hmm2 N 1) and 75 kg N / hm2 N 1, respectively. Applying medium level nitrogen fertilizer, 150 kg N / hm ~ (2) N _ (2); The abundance and community structure of soil total bacteria, ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing palaeomycetes (AOA) in wheat season surface layer (0-20 cm) in North China Plain were studied by applying high level nitrogen fertilizer, 225 kg N / hmm-2 N 3). The results showed that the total number of bacteria, AOB and AOA in soil ranged from 5.74 脳 10 ~ (-9) to 7.50 脳 10 ~ (10) ~ 9 ~ (9) C ~ (-1) 8.89 脳 10 ~ (6) ~ 2.66 脳 10 ~ (7) and 3.83 脳 10 ~ (8) 脳 10 ~ (8) 脳 10 ~ (8), respectively. The amount of AOA was higher than that of AOB, and the AOA/AOB value was between 81.72-14.38. Increasing nitrogen fertilizer significantly increased the number of AOB (P0.05), but had no significant effect on total bacteria and AOA (P0.05). Compared with CK, the number of AOB in N _ 1N _ 2 and N _ 3 was increased by 0.64% and 1.99 times, respectively. The community structure of AOB and AOA was significantly changed by nitrogen fertilizer application, and the difference of AOB community structure was greater in different nitrogen application treatments. Phylogenetic analysis showed that the soil AOB of nitrogen fertilized wheat was mainly Nitrosospira group, distributed in two branches of Cluster 3, and AOA was distributed in four branches of Cluster S. Correlation analysis showed that the amount of AOB was positively correlated with total nitrogen and ammonium nitrogen content, and negatively correlated with soil pH and C / N ratio (P0.05). There was a significant positive correlation between the amount of AOA and nitrate nitrogen content and soil pH content, and a significant negative correlation between the amount of AOA and ammonium nitrogen content (P0.05). The results showed that the amount of AOB and community structure of alkaline soil in North China Plain could be significantly changed by increasing nitrogen fertilizer. AOB in wheat soil was more sensitive to nitrogen fertilizer than AOA.
【作者單位】： 中國農(nóng)業(yè)大學(xué)農(nóng)學(xué)院;
【基金】：公益性行業(yè)(農(nóng)業(yè))科研專項(201503121-11) 河北省科技攻關(guān)項目(14227008D)
【分類號】：S154.3
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，本文編號：2387434
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