【摘要】：為探明生防菌SF1103、SF1104對(duì)黃瓜枯萎病菌的拮抗作用,采用平板對(duì)峙法、菌絲混合及凹玻片孢子培養(yǎng)法研究其拮抗能力,采用特異平板培養(yǎng)法分析生防菌的產(chǎn)酶及次生代謝產(chǎn)物,并通過盆栽試驗(yàn)分析其對(duì)生防菌的防效。結(jié)果表明,經(jīng)2株生防菌發(fā)酵液處理的病原菌的菌絲與孢子形態(tài)發(fā)生嚴(yán)重畸形并停止發(fā)育;2株生防菌均能分泌高活力的蛋白酶與纖維素酶,此外,SF1103具有解磷作用,SF1104能分泌嗜鐵素;盆栽試驗(yàn)表明,使用復(fù)合菌劑處理的出苗率較對(duì)照組提高23.3%,較化學(xué)防治組提高29.4%;發(fā)病率較無防治組下降25.9%,防效達(dá)到77.8%。生防菌SF1103、SF1104對(duì)黃瓜枯萎病菌具有較強(qiáng)的拮抗能力,不僅能有效防治黃瓜枯萎病,并且對(duì)黃瓜的出苗生長有促進(jìn)作用,有望進(jìn)一步開發(fā)為生防菌劑產(chǎn)品。
[Abstract]:In order to investigate the antagonism of SF1103,SF1104 against Fusarium wilt of cucumber, the antagonistic ability was studied by means of plate confrontation, mycelium mixing and concave glass spores culture, and the enzyme production and secondary metabolites of biocontrol bacteria were analyzed by specific plate culture. And through the pot experiment to analyze its biological control effect. The results showed that the mycelium and spore morphology of the pathogen treated with the fermentation broth of two strains of biocontrol bacteria were seriously deformed and stopped from developing. Both strains could secrete protease and cellulase with high activity. In addition, SF1103 could release phosphorus and SF1104 could secrete ferritin. Pot experiment showed that the emergence rate of the treated group was 23.3g higher than that of the control group, 29.4m higher than that of the chemical control group, and the incidence of the disease decreased 25.9%, and the control effect reached 77.8% compared with the control group. Biocontrol SF1103,SF1104 has a strong antagonistic ability against Fusarium wilt, which can not only effectively control cucumber wilt, but also promote the growth of cucumber seedling. It is expected to further develop the product of biological fungicides to control cucumber wilt.
【作者單位】： 陜西省生物農(nóng)業(yè)研究所陜西省酶工程技術(shù)研究中心中國科學(xué)院西北生物農(nóng)業(yè)中心;
【基金】：陜西省農(nóng)業(yè)科技創(chuàng)新計(jì)劃(2016NY-189) 西安市農(nóng)業(yè)科技創(chuàng)新計(jì)劃[NC1314(1)]~~
【分類號(hào)】：S436.421.13;S476.1
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本文編號(hào)：2374991