小麥赤霉病拮抗菌株篩選及其抑制作用研究
[Abstract]:A total of 27 strains of bacteria were isolated from the dung of wild yak in alpine meadow. The antagonistic strains of wheat scab were screened by plate confrontation method, and their inhibitory effects on (Fusarium gramminearum) of Fusarium graminearum were studied. The results showed that the antagonistic ability of strain LM3403 was the best among 8 wheat scab antagonists, and the inhibition bandwidth was (13.2 鹵0.8) mm.. Through morphological observation, physiological and biochemical analysis, 16s r DNA sequence analysis and gyr B gene sequence analysis, it was identified as Bacillus amylolyticus (Bacillus amyloliquefaciens). The results showed that the mycelium growth and conidial germination of Fusarium graminearum could be inhibited by LM3403, and the mycelium of Fusarium graminearum was deformed and the cell membrane was damaged. The strain also has the ability of synthesizing three kinds of antiseptic lipopeptide of surfactin,iturin,fengycin.
【作者單位】: 河南工業(yè)大學(xué)生物工程學(xué)院;
【基金】:國(guó)家自然科學(xué)基金(U1304332、31401543) 國(guó)家現(xiàn)代農(nóng)業(yè)(小麥)產(chǎn)業(yè)技術(shù)體系建設(shè)專(zhuān)項(xiàng)(CARS-03-01A) 河南省重點(diǎn)科技攻關(guān)項(xiàng)目(162102210043) 河南省高等學(xué)校青年骨干教師資助計(jì)劃項(xiàng)目(2012GGJS-086) 河南工業(yè)大學(xué)科技創(chuàng)新人才培育計(jì)劃項(xiàng)目(2013CXRC07)
【分類(lèi)號(hào)】:S435.121.45;S476.1
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