【摘要】：【目的】以典型南亞熱帶常綠闊葉林小坑林場(chǎng)土壤為研究對(duì)象,模擬2008年冰雪災(zāi)害對(duì)森林造成的損傷設(shè)置實(shí)驗(yàn),分析不同林冠開(kāi)度和凋落物輸入量對(duì)土壤固碳微生物群落結(jié)構(gòu)的影響�！痉椒ā吭囼�(yàn)設(shè)置對(duì)照(CN)、損傷處理+移除處理枝葉(TR)、損傷處理+保留處理枝葉(TD)、未處理+添加處理枝葉(UD)4個(gè)處理,受損處理一年后,采用MiSeq高通量測(cè)序技術(shù)對(duì)土壤固碳微生物的功能基因cbbL進(jìn)行測(cè)序分析�！窘Y(jié)果】通過(guò)生物信息學(xué)及統(tǒng)計(jì)學(xué)分析表明,森林林冠損傷后林冠開(kāi)度和凋落物輸入量增加,導(dǎo)致土壤固碳微生物種群數(shù)量降低,多樣性增加,群落結(jié)構(gòu)也受到影響,亞硝化螺菌屬(Nitrosospira)明顯增加,成為優(yōu)勢(shì)種群,而原來(lái)的優(yōu)勢(shì)菌群慢生根瘤菌屬(Bradyrhizobium)明顯減少。主成分分析(PCA)表明,與對(duì)照相比,其他3個(gè)樣地的土壤固碳微生物結(jié)構(gòu)均發(fā)生明顯改變。【結(jié)論】模擬林冠損傷處理一年后,凋落物的大量驟然輸入和林冠開(kāi)度增大提高了土壤固碳微生物群落多樣性,但降低了其種群數(shù)量,影響了土壤固碳微生物群落結(jié)構(gòu),這為進(jìn)一步的研究提供了依據(jù)。
[Abstract]:[objective] to simulate the experiment of forest damage caused by snow and ice disaster in 2008, taking the soil of the typical subtropical evergreen broadleaved forest Xiaokeng forest farm as the research object. The effects of different canopy opening and litter input on soil carbon-sequestration microbial community structure were analyzed. [methods] the control (CN), damage treatment was used to remove (TR), damage from branches and leaves, and (TD), of branches and leaves was preserved. The functional gene cbbL of soil carbon-sequestration microorganism was sequenced by MiSeq high-throughput sequencing technique after four treatments of (UD) were untreated and damaged for one year. [results] Bioinformatics and statistical analysis showed that the functional gene cbbL of soil carbon-sequestration microorganism was sequenced and analyzed by bioinformatics and statistical analysis. The canopy opening degree and litter input increased after forest canopy injury, which resulted in the decrease of soil carbon-sequestration microbial population, the increase of diversity and the influence of community structure. (Nitrosospira) increased obviously and became the dominant population. The (Bradyrhizobium) of the original dominant flora of Rhizobium (Bradyrhizobium) decreased significantly. Principal component analysis (PCA) showed that the soil carbon-sequestration microorganism structure of the other three plots changed significantly compared with the control. [conclusion] A year after simulated canopy damage treatment, The sudden input of litter and the increase of canopy opening increased the diversity of soil carbon-sequestration microbial community, but decreased its population number and affected the structure of soil carbon-fixation microbial community, which provided the basis for further research.
【作者單位】： 中國(guó)林業(yè)科學(xué)研究院熱帶林業(yè)研究所熱帶林業(yè)研究國(guó)家林業(yè)局重點(diǎn)實(shí)驗(yàn)室;
【基金】：中國(guó)林業(yè)科學(xué)研究院基本科研業(yè)務(wù)費(fèi)專項(xiàng)項(xiàng)目(No.CAFYBB2014MA003) 國(guó)家自然科學(xué)基金項(xiàng)目(No.31570544) 廣東省林業(yè)科技創(chuàng)新項(xiàng)目(No.2014KJCX019-01) 廣東自然科學(xué)基金項(xiàng)目(No.2014A030313727)~~
【分類號(hào)】：S714.3

【相似文獻(xiàn)】

相關(guān)期刊論文 前2條

1 白曉輝;張晶;楊勝天;李順江;李巍;李茜;盛浩然;;酸沉降對(duì)森林生長(zhǎng)固碳和土壤鹽基保持功能的影響[J];環(huán)境科學(xué)學(xué)報(bào);2010年01期

2 崔靜;陳云明;黃佳健;王瓊芳;姚志杰;張飛;;黃土丘陵半干旱區(qū)人工檸條林土壤固碳特征及其影響因素[J];中國(guó)生態(tài)農(nóng)業(yè)學(xué)報(bào);2012年09期

相關(guān)碩士學(xué)位論文 前1條

1 柳俊;內(nèi)蒙古草原土壤氮素形態(tài)及微生物群落結(jié)構(gòu)研究[D];浙江大學(xué);2016年

，

本文編號(hào)：2346574