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蠟蚧輪枝菌生物學(xué)特性及其與榕母管薊馬毒力的相關(guān)性

發(fā)布時(shí)間:2018-11-20 20:41
【摘要】:【目的】探討蠟蚧輪枝菌生物學(xué)特性與菌株對(duì)榕母管薊馬毒力的相關(guān)性,為榕母管薊馬優(yōu)質(zhì)高效生防菌株的初步篩選提供參考。【方法】室內(nèi)觀察蠟蚧輪枝菌V07、V16063、V3450和Vp28 4株菌株的生物學(xué)特性,測(cè)定其對(duì)榕母管薊馬成蟲(chóng)的毒力,通過(guò)逐步回歸分析探討兩者間的相關(guān)性。【結(jié)果】4株蠟蚧輪枝菌菌株的生物學(xué)特性存在異同,產(chǎn)孢量和孢子萌發(fā)率均很高,但V3450菌株生長(zhǎng)速度最快,孢子GT50最短;V16063和Vp28菌株生長(zhǎng)速度較快,孢子GT50較短;V07菌株生長(zhǎng)速度最慢,孢子GT50最長(zhǎng)。榕母管薊馬成蟲(chóng)受供試菌株侵染后的死亡趨勢(shì)均隨菌劑濃度升高和侵染時(shí)間延長(zhǎng)而上升,但不同菌劑中的變幅則明顯不同,侵染致死效果依次為V3450V16063Vp28V07,LT50依次為V3450V16063Vp28V07。逐步回歸分析表明,菌株生長(zhǎng)速度和孢子GT50對(duì)累計(jì)校正死亡率和LT50的作用均達(dá)顯著水平!窘Y(jié)論】在供試菌株產(chǎn)孢量和孢子萌發(fā)率均很高時(shí),菌株生長(zhǎng)速度和孢子GT50可作為評(píng)判蠟蚧輪枝菌對(duì)榕母管薊馬毒力大小的初篩指標(biāo)。
[Abstract]:[objective] to study the relationship between the biological characteristics and virulence of the strain to Ficus nativa thrips, and to provide a reference for the preliminary screening of high quality and high efficiency biocontrol strains of Ficus nativa thrips. [methods] to observe the virulence of Verticillium viride V07V 16063 in laboratory. The biological characteristics of four strains of V3450 and Vp28 were studied. The virulence of V3450 and Vp28 was determined. The correlation between them was studied by stepwise regression analysis. [results] the biological characteristics of 4 strains were similar and different. Spore production and spore germination rate were very high, but the growth rate of strain V3450 was the fastest and the spore GT50 was the shortest. The growth rate of V16063 and Vp28 was faster, the spore GT50 was shorter, the growth rate of V07 was the slowest and the spore GT50 was the longest. The mortality trend of adult Ficus thrips infected by the tested strains increased with the increase of the concentration of fungicides and the time of infection, but the variation range of different agents was obviously different, and the lethal effect of infection was V3450V16063Vp28V07LT50 and V3450V16063Vp28V in turn. Stepwise regression analysis showed that the growth rate of the strain and the effect of spores GT50 on cumulative corrected mortality and LT50 were significant. [conclusion] when the sporulation and spore germination rate of the tested strains were very high, The strain growth rate and spore GT50 can be used as a preliminary screening index to judge the virulence of rotifer of scale to Ficus nativa thrips.
【作者單位】: 福建省農(nóng)業(yè)科學(xué)院植物保護(hù)研究所;
【基金】:福建省科技重大專(zhuān)項(xiàng)(2013NZ0002-4) 福建省屬公益優(yōu)勢(shì)領(lǐng)域重點(diǎn)項(xiàng)目(2014R1024-1) 福建省自然科學(xué)基金項(xiàng)目(2014J01110) 福建省科技型中小企業(yè)技術(shù)創(chuàng)新資金項(xiàng)目(2015C0027)
【分類(lèi)號(hào)】:S476.12;S436.8

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