苜蓿根系分泌物對(duì)土壤難溶性磷活化作用的研究
發(fā)布時(shí)間：2018-11-15 17:17
【摘要】：選取6個(gè)苜蓿品種:騎士2、阿迪娜、TG3、騎士T、中苜1號(hào)和標(biāo)靶,于人工氣候箱中采用營(yíng)養(yǎng)液培養(yǎng)的方法,系統(tǒng)研究了缺磷和供磷條件下,苜蓿生長(zhǎng)至60 d、90 d、120 d的根系分泌物對(duì)土壤難溶性磷的活化效果及對(duì)磷素有效性的影響。結(jié)果如下:和對(duì)照相比,缺磷條件下,經(jīng)6個(gè)品種苜蓿根系分泌物處理后,土壤中磷素有效性提高;根系分泌物對(duì)磷酸二鈣(Ca2-P)的活化作用最顯著;對(duì)磷酸鋁鹽(Al-P)、磷酸鐵鹽(Fe-P)和磷酸八鈣(Ca8-P)均具有不同程度的活化作用;對(duì)磷酸十鈣(Ca10-P)和閉蓄態(tài)磷(O-P)無顯著活化效果。供磷條件下,具有類似的結(jié)果,但效果低于缺磷處理。缺磷及供磷條件下,Olsen-P與Ca2-P具有顯著或極顯著負(fù)相關(guān)關(guān)系,與Al-P具有顯著負(fù)相關(guān)關(guān)系,與O-P和Ca10-P無顯著相關(guān)關(guān)系;缺磷條件下與Ca8-P亦顯著負(fù)相關(guān),與Fe-P相關(guān)關(guān)系不顯著。由此可知,Ca2-P是苜蓿缺磷脅迫下Olsen-P的主要提供者,Al-P、Ca8-P亦可為植株提供Olsen-P,效果低于Ca2-P;Fe-P效果低于Al-P和Ca8-P。和對(duì)照相比,土壤經(jīng)缺磷培養(yǎng)60 d時(shí)的苜蓿根系分泌物處理后,Olsen-P含量及難溶性磷含量均有不同程度的變化。其中,TG3處理對(duì)土壤Olsen-P含量的提高幅度(11.98%)最為顯著,其次是騎士T的7.36%;對(duì)土壤Ca2-P的活化能力,表現(xiàn)突出的品種為TG3和騎士T,分別下降了24.99%和24.90%(P0.01),而阿迪娜、中苜1號(hào)和標(biāo)靶對(duì)Ca8-P的活化能力低于騎士2、TG3和騎士T。培養(yǎng)至90 d時(shí),騎士T的Olsen-P含量顯著(P0.05)高于除中苜1號(hào)和標(biāo)靶外的其他品種;騎士T、中苜1號(hào)和標(biāo)靶等3個(gè)品種處理對(duì)土壤Ca2-P的活化效果表現(xiàn)突出,土壤中Ca2-P含量除極顯著(P0.01)低于對(duì)照處理外,亦顯著(P0.05)低于其他品種處理;對(duì)Fe-P的活化效果表現(xiàn)較好的品種為TG3、騎士T和中苜1號(hào),但和其他品種處理間差異并不顯著(P0.05)。120 d時(shí),騎士T、中苜1號(hào)和標(biāo)靶的Olsen-P含量極顯著(P0.01)高于對(duì)照及其他品種處理;對(duì)Ca2-P的活化效果各品種處理均表現(xiàn)突出,極顯著低于對(duì)照(P0.01);對(duì)土壤Ca8-P的活化能力方面,騎士T表現(xiàn)相對(duì)較優(yōu),但與其他品種處理差異并不顯著(P0.05);標(biāo)靶對(duì)Al-P的活化能力表現(xiàn)相對(duì)突出,和對(duì)照相比其Al-P含量極顯著(P0.01)降低了28.49%。供磷條件下,60 d時(shí),TG3處理土壤Olsen-P含量顯著(P0.05)高于騎士2、阿迪娜和騎士T;對(duì)Ca2-P的活化效果最優(yōu)的品種為TG3;90 d時(shí),騎士T和中苜1號(hào)Olsen-P含量顯著(P0.05)高于騎士2和阿迪娜;對(duì)Ca2-P的活化效果以騎士T、中苜1號(hào)和標(biāo)靶最好,但各品種之間差異不顯著(P0.05);6個(gè)品種處理對(duì)Al-P、Fe-P和Ca8-P的活化效果無顯著性差異(P0.05)。120 d時(shí),騎士T和中苜1號(hào)的Olsen-P含量顯著(P0.05)高于除標(biāo)靶外的其他品種;騎士T的Ca2-P含量極顯著(P0.01)低于騎士2、阿迪娜和TG3;標(biāo)靶處理對(duì)Al-P的活化效果顯著(P0.05)高于除TG3和中苜1號(hào)外的其他3個(gè)品種處理。同一苜蓿品種,不同培養(yǎng)時(shí)間的根系分泌物對(duì)土壤Olsen-P含量的影響亦有所不同,表現(xiàn)為120 d90 d60 d,呈現(xiàn)出隨生長(zhǎng)時(shí)間的延長(zhǎng)土壤Olsen-P含量提高的趨勢(shì)。Ca2-P含量則表現(xiàn)為120 d90 d60 d,與60 d相比,缺磷條件培養(yǎng)至120 d時(shí),各品種處理的Ca2-P含量急劇下降,分別為76.34%、74.95%、74.92%、87.06%、88.67%、86.00%(P0.01)。Al-P、Fe-P和Ca8-P的含量變化趨勢(shì)為120 d90 d60 d。O-P和Ca10-P含量變化無顯著規(guī)律性。缺磷培養(yǎng)120 d時(shí),苜蓿根系分泌物對(duì)Ca2-P、Al-P、Fe-P、Ca8-P的活化效果優(yōu)于60 d和90 d。由此說明,隨著生長(zhǎng)天數(shù)的增加,根系分泌物對(duì)難溶性磷的活化作用愈加顯著。苜蓿在供磷和缺磷條件下的根系分泌物對(duì)土壤Ca2-P、Ca8-P、Al-P、Fe-P均具有一定的活化作用,缺磷條件下的活化效果高于供磷處理。6個(gè)品種中,騎士T根系分泌物相比于其他5個(gè)品種對(duì)提高土壤Olsen-P含量以及活化土壤中難溶性磷的效果最顯著。根系分泌物對(duì)土壤Olsen-P的活化量隨著植株生長(zhǎng)天數(shù)的增加而增加,活化效果為120 d90 d60 d。
[Abstract]:Six different varieties were selected: Knights 2, Adina, TG3, Knights T, Zhongjing No. 1 and the target. In the artificial climate box, the method of nutrient solution culture was used. The system studied the growth to 60d and 90d under the condition of phosphorus deficiency and phosphorus supply. The effect of the root exudates of 120d on the soil-insoluble phosphorus and the effect on the effectiveness of the phosphorus. The results were as follows: compared with the control, the effectiveness of the phosphorus in the soil was increased after the treatment of the root exudates of six varieties under the condition of phosphorus deficiency; the activation of the root exudates on the calcium phosphate (Ca2-P) was the most significant; and the aluminum phosphate (Al-P), The iron phosphate (Fe-P) and the calcium phosphate (Ca8-P) have different degree of activation, and no significant activation effect on the calcium phosphate (Ca10-P) and the closed-state phosphorus (O-P). Under the condition of phosphorus supply, similar results are obtained, but the effect is lower than that of the phosphorus-deficient treatment. Under the condition of phosphorus and phosphorus, Olsen-P had a significant or very significant negative correlation with Ca2-P, and there was a significant negative correlation with Al-P, and there was no significant correlation with O-P and Ca10-P. There was a significant negative correlation with Ca8-P under the condition of phosphorus deficiency, and the relationship with Fe-P was not significant. It can be found that Ca2-P is the main provider of Olsen-P under the condition of phosphorus and phosphorus, and the effect of Al-P and Ca8-P can also provide Olsen-P for the plant, and the effect is lower than that of Ca 2-P, and the Fe-P effect is lower than that of Al-P and Ca8-P. Compared with the control, the content of Olsen-P and the content of insoluble phosphorus varied in different degrees after the soil was treated with phosphorus-deficient culture for 60 days. Among them, the increase of the content of Olsen-P in the soil (11.98%) was the most significant, followed by the 7.36% of the Knight's T. The activity of the Ca 2-P in the soil showed that the variety was TG3 and the Knight T, which decreased by 24. 99% and 24. 90% (P0.01). The activation of Ca8-P was lower than that of Knights 2, TG3 and T. The Olsen-P content of the knight T was significantly higher than that of the other species except the medium (1) and the target (P0.05). The results showed that the Ca 2-P content in the soil was significantly lower than that of the control (P <0.01), and the content of Ca 2-P in the soil was lower than that of the control (P <0.01). The results showed that the content of Olsen-P was significantly higher than that of control and other varieties at the time of 120d. The results showed that the treatment of Ca 2-P was significantly lower than that of control (P0.01). The results showed that the activity of Ca8-P in the soil was relatively good, but the difference of the treatment with other varieties was not significant (P0.05); the activation ability of the target to the Al-P was relatively prominent, and the content of Al-P in the control group was significantly lower than that of the control (P0.01). Under the condition of phosphorus supply, the content of Olsen-P in TG3 was higher than that of Knights (P0.05). The effect of Ca 2-P on the activation of Ca 2-P was the best, but the difference between the varieties was not significant (P0.05). The effect of 6 varieties on the activation of Al-P, Fe-P and Ca8-P was not significant (P0.05). The content of Olsen-P was higher than that of the target (P0.05). The content of Ca 2-P was significantly lower than that of Knights 2, Adiana and TG3. The effect of target treatment on the activation of Al-P was significant (P0.05). The effect of root exudates on the content of Olsen-P in the soil was also different, and it was 120 d90 d60 d, which showed a tendency to increase the Olsen-P content in the soil along with the growth time. The content of Ca ~ (2-P) was 120 d90 d60 d, and the content of Ca 2-P in the various varieties decreased sharply, compared with that of 60d. The content of Ca 2-P in the various varieties was 74.34%, 74.95%, 74.92%, 87.6%, 80.67%, 80.00% (P0.01). Al-P, The change of the content of Fe-P and Ca8-P was 120 d90 d60 d. The content of O-P and Ca10-P had no significant regularity. The effect of root exudates on Ca 2-P, Al-P, Fe-P and Ca8-P was better than that of 60d and 90d. The root exudates under the condition of phosphorus and phosphorus deficiency have a certain activation effect on the soil Ca 2-P, Ca8-P, Al-P and Fe-P, and the activation effect under the condition of phosphorus deficiency is higher than that of the phosphorus supply treatment. Compared with the other five varieties, the root exudates of the Knights had the most significant effect on increasing the Olsen-P content of the soil and the hard-soluble phosphorus in the activated soil. The activation of Olsen-P in soil increased with the increase of the number of plant growth days, and the activation effect was 120 d90 d60 d.
【學(xué)位授予單位】：河北農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S153.6;S541
本文編號(hào)：2333940
[Abstract]:Six different varieties were selected: Knights 2, Adina, TG3, Knights T, Zhongjing No. 1 and the target. In the artificial climate box, the method of nutrient solution culture was used. The system studied the growth to 60d and 90d under the condition of phosphorus deficiency and phosphorus supply. The effect of the root exudates of 120d on the soil-insoluble phosphorus and the effect on the effectiveness of the phosphorus. The results were as follows: compared with the control, the effectiveness of the phosphorus in the soil was increased after the treatment of the root exudates of six varieties under the condition of phosphorus deficiency; the activation of the root exudates on the calcium phosphate (Ca2-P) was the most significant; and the aluminum phosphate (Al-P), The iron phosphate (Fe-P) and the calcium phosphate (Ca8-P) have different degree of activation, and no significant activation effect on the calcium phosphate (Ca10-P) and the closed-state phosphorus (O-P). Under the condition of phosphorus supply, similar results are obtained, but the effect is lower than that of the phosphorus-deficient treatment. Under the condition of phosphorus and phosphorus, Olsen-P had a significant or very significant negative correlation with Ca2-P, and there was a significant negative correlation with Al-P, and there was no significant correlation with O-P and Ca10-P. There was a significant negative correlation with Ca8-P under the condition of phosphorus deficiency, and the relationship with Fe-P was not significant. It can be found that Ca2-P is the main provider of Olsen-P under the condition of phosphorus and phosphorus, and the effect of Al-P and Ca8-P can also provide Olsen-P for the plant, and the effect is lower than that of Ca 2-P, and the Fe-P effect is lower than that of Al-P and Ca8-P. Compared with the control, the content of Olsen-P and the content of insoluble phosphorus varied in different degrees after the soil was treated with phosphorus-deficient culture for 60 days. Among them, the increase of the content of Olsen-P in the soil (11.98%) was the most significant, followed by the 7.36% of the Knight's T. The activity of the Ca 2-P in the soil showed that the variety was TG3 and the Knight T, which decreased by 24. 99% and 24. 90% (P0.01). The activation of Ca8-P was lower than that of Knights 2, TG3 and T. The Olsen-P content of the knight T was significantly higher than that of the other species except the medium (1) and the target (P0.05). The results showed that the Ca 2-P content in the soil was significantly lower than that of the control (P <0.01), and the content of Ca 2-P in the soil was lower than that of the control (P <0.01). The results showed that the content of Olsen-P was significantly higher than that of control and other varieties at the time of 120d. The results showed that the treatment of Ca 2-P was significantly lower than that of control (P0.01). The results showed that the activity of Ca8-P in the soil was relatively good, but the difference of the treatment with other varieties was not significant (P0.05); the activation ability of the target to the Al-P was relatively prominent, and the content of Al-P in the control group was significantly lower than that of the control (P0.01). Under the condition of phosphorus supply, the content of Olsen-P in TG3 was higher than that of Knights (P0.05). The effect of Ca 2-P on the activation of Ca 2-P was the best, but the difference between the varieties was not significant (P0.05). The effect of 6 varieties on the activation of Al-P, Fe-P and Ca8-P was not significant (P0.05). The content of Olsen-P was higher than that of the target (P0.05). The content of Ca 2-P was significantly lower than that of Knights 2, Adiana and TG3. The effect of target treatment on the activation of Al-P was significant (P0.05). The effect of root exudates on the content of Olsen-P in the soil was also different, and it was 120 d90 d60 d, which showed a tendency to increase the Olsen-P content in the soil along with the growth time. The content of Ca ~ (2-P) was 120 d90 d60 d, and the content of Ca 2-P in the various varieties decreased sharply, compared with that of 60d. The content of Ca 2-P in the various varieties was 74.34%, 74.95%, 74.92%, 87.6%, 80.67%, 80.00% (P0.01). Al-P, The change of the content of Fe-P and Ca8-P was 120 d90 d60 d. The content of O-P and Ca10-P had no significant regularity. The effect of root exudates on Ca 2-P, Al-P, Fe-P and Ca8-P was better than that of 60d and 90d. The root exudates under the condition of phosphorus and phosphorus deficiency have a certain activation effect on the soil Ca 2-P, Ca8-P, Al-P and Fe-P, and the activation effect under the condition of phosphorus deficiency is higher than that of the phosphorus supply treatment. Compared with the other five varieties, the root exudates of the Knights had the most significant effect on increasing the Olsen-P content of the soil and the hard-soluble phosphorus in the activated soil. The activation of Olsen-P in soil increased with the increase of the number of plant growth days, and the activation effect was 120 d90 d60 d.
【學(xué)位授予單位】：河北農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S153.6;S541
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相關(guān)期刊論文 前2條
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