【摘要】：[目的]模擬氣候變化背景下土壤酶活性季節(jié)變化規(guī)律和土壤酶活性對(duì)環(huán)境因子變異的響應(yīng),以深入理解氣候變化背景下微生物因素在調(diào)控土壤碳循環(huán)過程中的作用。[方法]以暖溫帶銳齒櫟林土壤為研究對(duì)象,采用紅外輻射增溫技術(shù)和96孔-微平板法測(cè)定了生長(zhǎng)季和非生長(zhǎng)季土壤水解酶和氧化酶活性,解析環(huán)境因子在調(diào)控土壤酶活性季節(jié)變異中的作用。[結(jié)果]顯示:增溫處理在生長(zhǎng)季顯著提高土壤溫度1.91℃,導(dǎo)致土壤有機(jī)碳含量顯著降低了12.15%,微生物量碳氮顯著增加了40.30%和61.29%;增溫處理在非生長(zhǎng)季顯著提高土壤溫度2.24℃,但對(duì)微生物量碳氮影響并不顯著。增溫處理對(duì)土壤專一酶活性的影響存在季節(jié)差異,生長(zhǎng)季除N-乙酰-葡糖苷酶外增溫處理降低了土壤專一酶活性;非生長(zhǎng)季除β-葡糖苷酶和蛋白酶外增溫處理增加了土壤專一酶活性。環(huán)境因子可以解釋土壤專一酶活性變異的82.5%,MBN和NH4-N極顯著影響土壤專一酶活性,二者共可以解釋變異的59.0%。[結(jié)論]MBN和NH4-N是影響土壤專一酶活性的主要環(huán)境因子,其他環(huán)境因子:土壤水熱配比、微生物量碳、微生物量碳氮比、溶解性有機(jī)碳和硝態(tài)氮等是影響土壤專一酶活性在處理間和季節(jié)間變異的主要驅(qū)動(dòng)因子。
[Abstract]:[objective] to simulate the seasonal variation of soil enzyme activity and the response of soil enzyme activity to the variation of environmental factors, in order to understand the role of microbial factors in the regulation of soil carbon cycle under the background of climate change. [methods] the activities of soil hydrolase and oxidase in growing season and non-growing season were determined by infrared radiation and 96 hole microplate method. The role of environmental factors in regulating seasonal variation of soil enzyme activity was analyzed. [results] the results showed that the soil temperature increased by 1.91 鈩，

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