不同磷水平下接種AM真菌對采煤塌陷區(qū)土壤GRSP量及玉米生長的影響
發(fā)布時(shí)間：2018-10-22 08:48
【摘要】：煤炭大量開采造成地面塌陷,使土壤結(jié)構(gòu)破壞,肥力下降,阻礙作物正常生長和對養(yǎng)分的吸收。叢枝菌根真菌(Arbuscular mycorrhizal fungi,AM真菌)在恢復(fù)退化的農(nóng)林生態(tài)系統(tǒng)中具有重要的生理功能,尤其促進(jìn)對土壤磷的吸收。通過盆栽試驗(yàn),研究4個(gè)施磷水平(0、25、50和100 mg/kg)下單接種摩西球囊霉(Glomus mosseae,G.m)、雙接種幼套球囊霉(Glomus etunicatum,G.e)與摩西球囊霉對采煤塌陷區(qū)土壤球囊霉素相關(guān)土壤蛋白(GRSP)、玉米生長及地上部磷吸收的影響。結(jié)果表明,接種與磷水平及他們之間的交互作用對AM真菌叢枝豐度、株高、莖粗和易提取球囊霉素相關(guān)土壤蛋白(EE-GRSP)量具有顯著影響(P0.05);接種處理AM真菌叢枝豐度在0.29%~25.22%之間,同一磷水平雙接種處理顯著高于單接種處理(P0.05)。而且GRSP量與AM真菌叢枝豐度表現(xiàn)出顯著的二次曲線相關(guān)。相同磷水平下,總球囊霉素相關(guān)土壤蛋白(T-GRSP)和易提取球囊霉素相關(guān)土壤蛋白(EE-GRSP)量均呈雙接種≥單接種不接種,同一接種處理GRSP量隨磷水平提高先升后降。雖然單接種和不接種處理在不同磷水平間對玉米生長的影響差異不顯著,但是雙接種處理顯著提高了玉米株高、莖粗、地上部干質(zhì)量及地上部吸磷量,表現(xiàn)為50、100 mg/kg磷水平顯著高于0、25 mg/kg磷水平;相關(guān)性分析表明,AM真菌叢枝豐度與GRSP量、株高、莖粗、地上部磷量呈顯著正相關(guān)。
[Abstract]:Large amount of coal mining results in ground collapse, soil structure destruction and fertility decline, which hinder the normal growth of crops and nutrient absorption. Arbuscular mycorrhizal fungi (Arbuscular mycorrhizal fungi,AM) play an important role in the restoration of degraded agroforestry ecosystem, especially in promoting the absorption of soil phosphorus. Through pot experiments, The effects of four phosphorus application levels (0 ~ 25 ~ (50) and 100 mg/kg) on the growth and phosphorus uptake of (GRSP), corns, a soil protein associated with Bleomycin, were studied by placing orders for inoculation of Glomus mosseae,G.m and double inoculation of Glomus etunicatum,G.e and Mosaic Balloon in the soil of coal mining collapse area. The results showed that inoculation and phosphorus level and their interaction had significant effects on the AM fungi branch abundance, plant height, stem diameter and the amount of EE-GRSP (P0.05), and the branching abundance of AM fungi was between 0.29% and 25.22%. The treatment of double inoculation at the same phosphorus level was significantly higher than that of single inoculation (P0.05). Moreover, the GRSP content was significantly correlated with the branch abundance of AM fungi. Under the same phosphorus level, the amount of total balloon mycin associated soil protein (T-GRSP) and easy to extract balloon mycin associated soil protein (EE-GRSP) were all double inoculated 鈮，
本文編號：2286686
[Abstract]:Large amount of coal mining results in ground collapse, soil structure destruction and fertility decline, which hinder the normal growth of crops and nutrient absorption. Arbuscular mycorrhizal fungi (Arbuscular mycorrhizal fungi,AM) play an important role in the restoration of degraded agroforestry ecosystem, especially in promoting the absorption of soil phosphorus. Through pot experiments, The effects of four phosphorus application levels (0 ~ 25 ~ (50) and 100 mg/kg) on the growth and phosphorus uptake of (GRSP), corns, a soil protein associated with Bleomycin, were studied by placing orders for inoculation of Glomus mosseae,G.m and double inoculation of Glomus etunicatum,G.e and Mosaic Balloon in the soil of coal mining collapse area. The results showed that inoculation and phosphorus level and their interaction had significant effects on the AM fungi branch abundance, plant height, stem diameter and the amount of EE-GRSP (P0.05), and the branching abundance of AM fungi was between 0.29% and 25.22%. The treatment of double inoculation at the same phosphorus level was significantly higher than that of single inoculation (P0.05). Moreover, the GRSP content was significantly correlated with the branch abundance of AM fungi. Under the same phosphorus level, the amount of total balloon mycin associated soil protein (T-GRSP) and easy to extract balloon mycin associated soil protein (EE-GRSP) were all double inoculated 鈮，
本文編號：2286686
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