【摘要】：采用添加NaH_2PO_4的方法調(diào)節(jié)豬場污水中氮磷比(N∶P)分別為8∶1、16∶1、32∶1和64∶1,以未添加NaH_2PO_4的污水為對照(氮磷比為532∶1),探討一株耐污綠球藻(Chlorococcum sp.)在不同氮磷比污水中的生長性能及其對豬場污水(初始氨氮濃度為291.31 mg·L~(-1))的凈化效果.結(jié)果表明:經(jīng)過12 d的培養(yǎng),綠球藻(接種密度為400×104cells·m L~(-1))在N∶P為64∶1的污水中生長最好,且對污水中氨態(tài)氮和總氮的去除效果最佳,細(xì)胞密度和生物量分別為3393×104cells·m L~(-1)和0.49 g·L~(-1),對氨態(tài)氮和總氮的去除率分別為74.94%和48.78%,顯著高于對照組,氨態(tài)氮濃度降低到73.01 mg·L~(-1),總氮濃度降低到148.96 mg·L~(-1).培養(yǎng)期間各試驗組污水中硝態(tài)氮濃度均升高.培養(yǎng)12 d后,N∶P為64∶1組污水中總磷濃度降低為3.07 mg·L~(-1),去除率為71.86%.綜上,綠球藻在N∶P為64∶1的污水中生長性能及其對污水中氨態(tài)氮和總氮的去除效果均最佳,可使污水中的氨態(tài)氮和總磷濃度基本達(dá)到相關(guān)排放標(biāo)準(zhǔn).
[Abstract]:The ratio of N to P (N: P) in wastewater of pig farm was adjusted by adding NaH_2PO_4 to 8: 1: 16: 1: 32: 1 and 64: 1, respectively. Using the wastewater without NaH_2PO_4 as control (nitrogen / phosphorus ratio was 532: 1), a stain tolerant Chlorella aeruginosa (Chlorococcum sp.) strain was studied. The growth performance and purification effect of pig farm wastewater (initial ammonia nitrogen concentration of 291.31 mg L ~ (-1) in wastewater with different nitrogen and phosphorus ratios were studied. The results showed that Chlorella aeruginosa (inoculation density was 400 脳 104cells mL ~ (-1) grew best in the wastewater with N: P = 64:1, and the removal efficiency of ammonia nitrogen and total nitrogen was the best after 12 days culture. The cell density and biomass were 3393 脳 104cells mL ~ (-1) and 0.49 g / L ~ (-1), respectively. The removal rates of ammonia nitrogen and total nitrogen were 74.94% and 48.78%, respectively, which were significantly higher than those of the control group. The ammonia nitrogen concentration decreased to 73.01 mg L ~ (-1) and the total nitrogen concentration to 148.96 mg L ~ (-1). During the culture period, the concentration of nitrate nitrogen in the sewage of each experimental group increased. After 12 days of culture, the total phosphorus concentration in the effluent of the 64:1 group was reduced to 3.07 mg L ~ (-1), and the removal rate was 71.86%. In conclusion, the growth performance of Chlorophyta in the wastewater with N: P = 64:1 and the removal efficiency of ammonia nitrogen and total nitrogen in wastewater are the best, and the concentration of ammonia nitrogen and total phosphorus in sewage can basically reach the relevant discharge standard.
【作者單位】： 上海海洋大學(xué)農(nóng)業(yè)部淡水水產(chǎn)種質(zhì)資源重點實驗室;上海市水產(chǎn)養(yǎng)殖工程技術(shù)研究中心;水產(chǎn)動物遺傳育種中心上海市協(xié)同創(chuàng)新中心(ZF1206);
【基金】：上海市科技興農(nóng)項目[滬農(nóng)科攻字(2015)第1-2號]~~
【分類號】：X173;X713

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