【摘要】：運(yùn)用宏基因組技術(shù)研究不同比例鋸末牛糞堆肥產(chǎn)物中微生物的組成和分布。結(jié)果表明,牛糞與鋸末質(zhì)量比為2∶1、3∶1、4∶1的3個堆肥樣本中微生物可操作分類單元(operational taxonomic units,OTU)有較大的差異,其中含量最高的3個門的微生物為變形菌門、擬桿菌門和厚壁菌門,在不同樣本中它們的含量也有較大差異。從屬水平分析,含量最高的是甲基單胞菌屬、噬氫菌屬、固氮菌屬和寡養(yǎng)單胞菌等營養(yǎng)轉(zhuǎn)化和合成的菌屬。降解纖維素和木質(zhì)素的也有一定的含量,特別是假單胞菌屬含量較高。經(jīng)過對堆肥產(chǎn)物基本性質(zhì)及其微生物的分析發(fā)現(xiàn),當(dāng)牛糞與鋸末的比例為2∶1時,堆肥樣品中的營養(yǎng)轉(zhuǎn)化和合成菌屬以及去除污染菌屬都高于其他2個樣品,堆肥效果較好。
[Abstract]:The composition and distribution of microorganisms in different proportion of sawdust cattle manure compost were studied by macrogenome technique. The results showed that there was significant difference among the three compost samples with the mass ratio of cow dung to sawdust of 2: 1: 3: 1: 4: 1. The microbial operable taxonomy unit (operational taxonomic units,OTU) of the three compost samples with the highest content was Proteus. The content of Bacteroides phylum and phylum thuringiensis in different samples were also different. According to the analysis of subordinate level, the highest content is the genus Methylomonas, Hydrocytoma, Azotobacter and Oligotrophomonas. The content of cellulose and lignin was also high, especially in Pseudomonas. By analyzing the basic properties of compost products and their microbes, it was found that when the ratio of cow manure to sawdust was 2:1, the genera of nutrient transformation, synthetic bacteria and decontamination bacteria in compost samples were higher than those of the other two samples, and the composting effect was better.
【作者單位】： 文華學(xué)院環(huán)境工程系;
【基金】：湖北省省級戰(zhàn)略新興(支柱)產(chǎn)業(yè)人才培養(yǎng)計劃——環(huán)境工程(編號:j0900760102)
【分類號】：S141.4

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