【摘要】：采用富集培養(yǎng)法,從新疆棉田土壤中分離篩選二甲戊靈高效降解菌株,研究不同培養(yǎng)條件對二甲戊靈降解的影響,以期為二甲戊靈污染土壤的治理提供依據(jù)。結(jié)果表明,經(jīng)平板法初篩獲得9株高度耐受二甲戊靈的降解細菌,其中,菌株JY-2和JY-5在3 d后對100 mg/L二甲戊靈的降解率在75%以上。此2株細菌經(jīng)形態(tài)觀察和16S rDNA序列分析,被初步鑒定為沙雷氏菌(Serratia sp.)和假單胞菌(Pseudomonas sp.)。2株細菌均能在以二甲戊靈為唯一碳源的無機鹽培養(yǎng)基中生長,其在外加碳源量0.5%、菌株接種量10%、初始二甲戊靈質(zhì)量濃度200 mg/L、pH值7.0、溫度30℃的最佳培養(yǎng)條件下振蕩培養(yǎng)3 d,對二甲戊靈的降解率分別可達83.34%和82.79%�？梢�,此2株細菌有較強的適應能力,能高效、快速地降解二甲戊靈。
[Abstract]:In order to provide the basis for the treatment of the contaminated soil of Xinjianwuling, we isolated and screened the high-efficient degrading strains of dimethylpentyl from cotton soil in Xinjiang by means of enrichment and culture, and studied the effect of different culture conditions on the degradation of dimethopentalin in order to provide the basis for the treatment of the contaminated soil. The results showed that 9 strains of high tolerance to dimethopentalin were obtained by plate method. The degradation rate of 100 mg/L penicillin by JY-2 and JY-5 was more than 75% after 3 days. The two strains were identified as (Serratia sp.) by morphological observation and 16s rDNA sequence analysis. Both Pseudomonas and Pseudomonas (Pseudomonas sp.). 2 strains could grow in inorganic salt medium with dimethylpentalin as the sole carbon source. The degradation rates of dimethylpentalin were 83.34% and 82.79%, respectively, under the optimum culture conditions of 0.5 carbon source, 10 inoculations, 7.0 mg/L,pH and 30 鈩，

本文編號：2232941