【摘要】：【目的】探索雙委夜蛾Athetis dissimilis(Hampson)的耐寒策略及越冬蟲態(tài)�！痉椒ā恳允覂�(nèi)雙委夜蛾種群為蟲源,分別測(cè)定3齡、4齡、5齡、6齡和老熟幼蟲以及蛹的過冷卻點(diǎn)(supercooling point,SCP)、結(jié)冰點(diǎn)(freezing point,FP)、含水量以及脂肪、蛋白質(zhì)、甘油、山梨醇和海藻糖含量�！窘Y(jié)果】不同齡期幼蟲和蛹之間過冷卻點(diǎn)和結(jié)冰點(diǎn)存在顯著差異,低齡幼蟲SCP和FP較高;隨著齡期增長(zhǎng),二者均降低,老熟幼蟲的SCP和FP最低,分別為-18.20℃和-11.72℃。不同齡期幼蟲和蛹的生化物質(zhì)含量均存在顯著差異。隨著齡期的增加,含水量和甘油含量呈降低的趨勢(shì),蛹的含水量(65.33%)和甘油含量(154.90μg/mg)最低;脂肪和蛋白質(zhì)含量隨齡期的增加呈現(xiàn)增加的趨勢(shì),蛹的脂肪和蛋白質(zhì)含量最高,分別為25.36%和2 298.37μg/mg。老熟幼蟲海藻糖含量相對(duì)較高(57.28 mg/g),而山梨醇含量較低(15.49 mg/g)�！窘Y(jié)論】與其他齡期相比,雙委夜蛾老熟幼蟲和蛹的耐寒性最強(qiáng),是最可能的越冬蟲態(tài);脂肪、蛋白質(zhì)和海藻糖可能是雙委夜蛾重要的耐寒物質(zhì)。
[Abstract]:[objective] to explore the cold tolerance strategy and overwintering state of Athetis dissimilis (Hampson). The supercooling point (supercooling), freezing point (FP), fat, protein, glycerin, fat, protein and glycerin were measured at the 3rd and 4th instar, 5th instar and 6th instar and pupae, respectively. [results] there were significant differences in supercooling point and freezing point between larvae and pupae of different ages, SCP and FP of young larva were higher, and SCP and FP of mature larvae were the lowest with the increase of age. It was -18.20 鈩，

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