【摘要】：【目的】利用表皮碳?xì)浠衔镞M(jìn)行昆蟲鑒定是目前昆蟲分類學(xué)中非�；钴S的研究方向之一。昆蟲表皮碳?xì)浠衔锏囊后w浸提及進(jìn)樣技術(shù)是GC-MS常規(guī)的進(jìn)樣方法之一,隨著現(xiàn)代技術(shù)的發(fā)展,固體進(jìn)樣也被廣泛地用于昆蟲表皮碳?xì)浠衔锏蔫b定及昆蟲的分類與進(jìn)化分析。本研究探索利用固體進(jìn)樣法開展實(shí)蠅表皮碳?xì)浠衔锏奶崛∨c分析,探索并獲得簡(jiǎn)便可行的取樣與進(jìn)樣技術(shù),為檢疫性實(shí)蠅的快速鑒定提供新方法�！痉椒ā恳越坌�(shí)蠅Bactrocera dorsalis(Hendel)成蟲為材料,采用固體進(jìn)樣與液體浸提進(jìn)樣方法,開展實(shí)蠅表皮碳?xì)浠衔锝M成成分鑒定與分析;比較獲得桔小實(shí)蠅成蟲取樣部位,并分析標(biāo)本保存時(shí)間對(duì)桔小實(shí)蠅表皮碳?xì)浠衔锝M成的影響�！窘Y(jié)果】利用固體進(jìn)樣技術(shù)獲得的桔小實(shí)蠅翅表皮碳?xì)浠衔锓N類(39)較液體進(jìn)樣的(36)略多,且翅和中足的表皮碳?xì)浠衔锖糠謩e為0.18~23.37和0.03~4.13 ng/μg,極顯著地高于用液體進(jìn)樣法檢測(cè)到相同部位的化合物含量(分別為0.05~3.93和0.01~0.68 ng/μg)(P0.01),而且前者的檢測(cè)靈敏度是后者的1.7~10.8倍;同時(shí),翅樣品的表皮碳?xì)浠衔锖匡@著地高于足中的含量(P0.01)。利用固體進(jìn)樣獲得的桔小實(shí)蠅成蟲翅、足或胸部的表皮碳?xì)浠衔飯D譜與對(duì)照處理所得圖譜相似。干燥保存30 d的桔小實(shí)蠅翅的碳?xì)浠衔锏姆N類及含量與新鮮標(biāo)本(保存0 d)無顯著性差異(P0.05);但保存30,60和150 d標(biāo)本的碳?xì)浠衔锎嬖陲@著差異(P0.05),主要是含量的差異。【結(jié)論】研究表明,利用固體進(jìn)樣檢測(cè)昆蟲表皮碳?xì)浠衔锏撵`敏度較高,而且桔小實(shí)蠅成蟲的主要表皮碳?xì)浠衔锓N類較穩(wěn)定,因此可以作為我國口岸檢疫性實(shí)蠅表皮碳?xì)浠衔铽@取與分析的一種新方法,而且檢測(cè)中翅是固體進(jìn)樣的較合適取樣部位。
[Abstract]:[objective] to identify insects using epidermal hydrocarbons is one of the most active research directions in insect taxonomy. The liquid extraction and sampling technique of insect epidermis hydrocarbon is one of the conventional methods of GC-MS. With the development of modern technology, solid injection has been widely used in the identification of insect epidermal hydrocarbons and the classification and evolution analysis of insects. In this study, the extraction and analysis of carbohydrates from the epidermis of the fly were carried out by solid sampling method, and a simple and feasible sampling and sampling technique was obtained. To provide a new method for rapid identification of quarantine fruit fly. [methods] using Bactrocera dorsalis (Hendel) adult of fruit fly as material, solid sample and liquid extraction method were used to identify and analyze the composition of hydrocarbon in the epidermis of fruit fly. The sampling sites of adult fruit fly were compared. The effects of preservation time on the composition of carbohydrates in the epidermis of the fruit fly were analyzed. [results] the species of hydrocarbon in the epidermis of the fly wings (39) obtained by solid injection technique were slightly more than those in the liquid sample (36). The contents of hydrocarbon in the epidermis of wing and middle foot were 0.18 渭 g and 0.03 渭 g, respectively, which were significantly higher than those in the same site detected by liquid injection (0.053.93 and 0.01g / 0.68 ng/ g) (P0.01, respectively), and the sensitivity of the former was 1.710.8 times that of the latter, and the sensitivity of the former was 1.710.8 times higher than that of the latter, and the sensitivity of the former was 1.710.8 times higher than that of the latter, and the sensitivity of the former was 1.710.8 times that of the latter. The content of epidermal hydrocarbons in wing samples was significantly higher than that in feet (P0.01). The epidermal hydrocarbon maps of adult wings, feet or chest of orange fruit fly obtained by solid injection were similar to those obtained from the control treatment. There was no significant difference between the species and content of hydrocarbon in the wings of fruit fly preserved for 30 days (P0.05), but there was a significant difference in hydrocarbon content between the specimens of 300.60 and 150 days (P0.05), mainly the difference of the content of hydrocarbon between the fresh specimens (P05) and the fresh specimens (P05). [conclusions] study shows that The sensitivity of solid injection for the detection of insect epidermal hydrocarbons was high, and the main epidermal hydrocarbon species of adult orange-fly were stable. Therefore, it can be used as a new method for obtaining and analyzing epidermis hydrocarbons of quarantine fly in our country, and the detection of middle wing is a more suitable sampling site for solid injection.
【作者單位】： 廣東省農(nóng)業(yè)科學(xué)院植物保護(hù)研究所廣東省植物保護(hù)新技術(shù)重點(diǎn)實(shí)驗(yàn)室;華南農(nóng)業(yè)大學(xué)農(nóng)學(xué)院;廣東檢驗(yàn)檢疫技術(shù)中心;
【基金】：廣州市科技計(jì)劃項(xiàng)目(201510010232) 國家科技支撐計(jì)劃課題(2015BAD08) 科技部科技伙伴計(jì)劃(KY201402015)
【分類號(hào)】：S433

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