【摘要】：內(nèi)蒙古次生林區(qū)是我國(guó)森林資源的重要組成部分,在我國(guó)的生態(tài)環(huán)境建設(shè)以及區(qū)域生物地球化學(xué)循環(huán)中起著非常重要的作用。土壤微生物是森林生態(tài)系統(tǒng)的重要組成部分,連接著森林地表植被與地下土壤養(yǎng)分系統(tǒng),在維持生態(tài)系統(tǒng)的平衡過(guò)程中起著重要作用。但是到目前為止對(duì)內(nèi)蒙古次生林區(qū)土壤碳氮及微生物群落動(dòng)態(tài)的研究甚少。本研究選擇大青山常見(jiàn)樹(shù)種,運(yùn)用氯仿熏蒸浸提法和實(shí)時(shí)熒光定量PCR分析方法研究了春秋兩季內(nèi)蒙古呼和浩特市大青山小井溝扁桃(Amygdalus sp.)、油松(Pinus tabulaegormis)、白樺(Betula platyphylla)、山楂(Crataegus pinnatifida)、小檗(Berberisthumbergii)、椴樹(shù)(Tilia.monolica)、楓樹(shù)(Acer sp.)、虎榛子(Ostryopsis davidiana)、蒙古櫟(Quercus mongolica)、繡線菊(Spiraea sp.)、華北落葉松(Larix principis-rupprechtii)11種根圍土壤微生物量碳氮與微生物豐度。研究結(jié)果如下：春季不同樹(shù)種根圍土壤微生物量碳變化范圍為360mg/kg-984mg/kg,微生物量氮變化范圍為63mg/kg-178mg/kg,細(xì)菌16S rRNA基因拷貝數(shù)變化范圍為每克干土中8.8×108-3.6×109個(gè)拷貝數(shù),真菌ITS基因拷貝數(shù)變化范圍為81×107-6.3×108個(gè)拷貝數(shù)：秋季不同樹(shù)種根圍土壤微生物量碳變化范圍為818mg/kg-1342mg/kg,微生物量氮變化范圍為217mg/kg-240mg/kg,細(xì)菌16S rRNA基因拷貝數(shù)變化范圍為每克干土中1.7×109-4.1×109個(gè)拷貝數(shù),真菌ITS基因拷貝數(shù)變化范圍為5.8×108-1.7×109個(gè)拷貝數(shù)。通過(guò)結(jié)果分析可知,季節(jié)和樹(shù)種對(duì)土壤微生物量碳氮與微生物豐度具有顯著影響作用,而且,土壤微生物量碳氮含量與土壤微生物豐度顯著正相關(guān)。
[Abstract]:The secondary forest region of Inner Mongolia is an important part of forest resources in China, which plays an important role in the construction of ecological environment and the regional biogeochemical cycle in China. Soil microorganism is an important component of forest ecosystem, which connects the forest surface vegetation with the soil nutrient system, and plays an important role in the process of maintaining the balance of the ecosystem. However, there are few studies on soil carbon, nitrogen and microbial community dynamics in Inner Mongolia secondary forest. In this study, the common tree species in Daqing Mountain were selected. Chloroform fumigation extraction method and real-time fluorescence quantitative PCR analysis were used to study the effects of spring and autumn on Pinus tabulaegormis), Bai Hua (Crataegus pinnatifida), berberi, Tilia.monolica) and (Acer sp.), tiger hazel of Almond (Pinus tabulaegormis), Bai Hua) in Daqingshan Xiaojinggou, Huhhot City, Inner Mongolia in spring and autumn. 11 species of Spiraea sp.), Larix principis-rupprechtii (Larix principis-rupprechtii) have microbial biomass, carbon, nitrogen and abundance in the rhizosphere soil of Ostryopsis davidiana), (Quercus mongolica), stitch chrysanthemum). The results are as follows: the range of microbial biomass carbon variation is 360 mg / kg-984 mg / kg, microbial biomass nitrogen varies from 63 mg / kg to 178 mg / kg in spring, and the variation range of bacterial 16s rRNA gene copy number is 8.8 脳 108-3.6 脳 109 copies per gram dry soil in spring. The variation range of its gene copy number of fungi is 81 脳 107-6.3 脳 108 copies. The range of microbial biomass carbon variation is 818mg / kg-1342mg / kg, microbial biomass nitrogen variation range is 217mg / kg-240mg / kg, bacterial 16s rRNA gene copy number varies from 1.7 脳 109-4.1 脳 109 copies per gram dry soil in autumn, and the variation range of microbial biomass nitrogen is 217mg / kg-240mg / kg, and the variation range of bacterial 16s rRNA gene copy number is 1.7 脳 109-4.1 脳 109 copies per gram dry soil in autumn. The copy number of its gene varied from 5.8 脳 10 ~ (8) to 1.7 脳 10 ~ (9) copies in fungi. The results showed that season and tree species had significant effects on soil microbial biomass carbon nitrogen and microbial abundance, and soil microbial biomass carbon nitrogen content was positively correlated with soil microbial abundance.
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S714.3

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本文編號(hào)：2133220