本文選題：茶角胸葉甲 + 球孢白僵菌��； 參考：《茶葉科學(xué)》2017年03期

【摘要】：茶角胸葉甲(Basilepta melanopus Lefevre)是一種茶樹上常見的食葉性害蟲,其發(fā)生為害嚴(yán)重影響茶葉的產(chǎn)量和品質(zhì)。近年來,該蟲在局部茶園,尤其是有機茶園中常爆發(fā)成災(zāi)。如何有效防治該害蟲,已成為亟待解決的問題。本研究選擇原寄主為茶園鞘翅目害蟲的9個球孢白僵菌(Beauveria bassiana)菌株,依據(jù)菌落形態(tài)、菌株生長速率、產(chǎn)孢量、孢子萌發(fā)率、耐熱力和抗紫外能力等生物學(xué)性狀初步篩選出Bb338、Bb346和Bb2-1等3個生物學(xué)性狀優(yōu)良的菌株;在此基礎(chǔ)上,利用浸蟲法研究了3個菌株對茶角胸葉甲成蟲的殺蟲毒力。生測結(jié)果表明,用每毫升1×10~7個孢子的孢懸液處理后,球孢白僵菌Bb2-1對茶角胸葉甲成蟲的累計校正死亡率達100%,僵蟲率達86.11%;LT_(50)僅為3.32 d。綜合上述各生物學(xué)特性和殺蟲毒力表明,篩選到的Bb2-1菌株具有生長速率快、產(chǎn)孢量大、耐熱力和抗紫外能力強,以及對茶角胸葉甲殺蟲毒力最強和殺蟲速率最快等優(yōu)點,在今后茶角胸葉甲的生物防治中將發(fā)揮重要的作用。
[Abstract]:Basilepta melanopus Lefevre is a common leaf-eating pest in tea plants. Its occurrence seriously affects the yield and quality of tea. In recent years, the insect often erupts in local tea garden, especially in organic tea garden. How to effectively control this pest has become an urgent problem. In this study, 9 Beauveria bassiana (Beauveria bassiana) strains, which were originally hosted by Coleoptera pests in tea garden, were selected. According to the morphology of colony, the growth rate, the amount of spores, the germination rate of spores. Three strains with excellent biological properties, such as Bb338Bb346 and Bb2-1, were preliminarily screened for their thermal and ultraviolet resistance, and the insecticidal toxicity of the three strains to the adult beetles of the tea horn was studied by the method of soaking insects. The bioassay results showed that after treated with spore suspension of 1 脳 10 ~ 7 spores per milliliter, the cumulative corrected mortality of Beauveria bassiana Bb2-1 was 100 and the rate of Metarhizium anisopliae was only 3.32 days. The biological characteristics and insecticidal virulence showed that the screened strain Bb2-1 had the advantages of fast growth rate, high sporulation production, strong heat resistance and UV resistance, and the strongest insecticidal toxicity and the fastest insecticidal rate to the tea leaf beetle. In the future, it will play an important role in biological control.
【作者單位】： 福建省農(nóng)業(yè)科學(xué)院茶葉研究所;廣東省農(nóng)業(yè)科學(xué)院飲用植物研究所/廣東省茶樹資源創(chuàng)新利用重點實驗室;
【基金】：福建省自然科學(xué)基金項目(2015J01099) 國家茶葉產(chǎn)業(yè)技術(shù)體系項目(CARS-23) 福建省農(nóng)業(yè)科學(xué)院茶葉研究所重點項目(2014-cys-02)
【分類號】：S435.711;S476.12

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