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重金屬與抗生素對(duì)SBBR中微生物活性的影響

發(fā)布時(shí)間:2018-07-04 17:39

  本文選題:養(yǎng)豬廢水 + SBBR。 參考:《南昌大學(xué)》2015年碩士論文


【摘要】:水污染問(wèn)題日益嚴(yán)重,在眾多的水環(huán)境污染中,畜牧養(yǎng)殖業(yè)造成的污染是不可忽視的,其中尤以養(yǎng)豬廢水污染為甚。重金屬和抗生素被養(yǎng)豬行業(yè)廣泛利用。大量重金屬和抗生素跟隨排泄物進(jìn)入廢水處理系統(tǒng),為后續(xù)的廢水處理增添了不少難度。本研究旨在探究重金屬和抗生素對(duì)污水處理系統(tǒng)中SBBR反應(yīng)器中的微生物的影響,為養(yǎng)豬場(chǎng)廢水處理奠定一定的理論基礎(chǔ)。通過(guò)在正常運(yùn)行的SBBR反應(yīng)器中投加不同濃度以銅、鋅為代表的重金屬和以土霉素、磺胺間甲氧嘧啶為代表的抗生素,探究其對(duì)微生物的影響。得到結(jié)果如下:(1)銅濃度小于2mg/L時(shí),銅能促進(jìn)微生物呼吸作用,且濃度2mg/L銅的促進(jìn)作用大于濃度1mg/L銅和濃度0.5mg/L銅的促進(jìn)作用。銅濃度大于4mg/L時(shí),銅能抑制微生物呼吸作用,且濃度16mg/L銅的抑制作用大于濃度4mg/L銅和濃度8mg/L銅的抑制作用。鋅濃度小于4mg/L時(shí),鋅能促進(jìn)微生物呼吸作用,且濃度4mg/L鋅的促進(jìn)作用大于濃度2mg/L鋅和濃度1mg/L鋅的促進(jìn)作用。鋅濃度大于8mg/L時(shí),鋅能抑制微生物呼吸作用,且濃度32mg/L鋅的抑制作用大于濃度16mg/L鋅和濃度8mg/L鋅的抑制作用。(2)銅濃度小于8mg/L時(shí)會(huì)刺激微生物的代謝,只有銅濃度大于16mg/L時(shí)才會(huì)抑制微生物的代謝。鋅濃度小于16mg/L時(shí)會(huì)刺激微生物的代謝,只有鋅濃度大于32mg/L時(shí)才會(huì)抑制微生物的代謝。說(shuō)明銅對(duì)微生物代謝的影響比鋅對(duì)微生物代謝的影響要強(qiáng)烈。銅鋅復(fù)合對(duì)微生物呼吸和代謝表現(xiàn)出協(xié)同抑制作用。銅鋅復(fù)合會(huì)加大單因素銅鋅對(duì)微生物的毒性。高濃度的銅會(huì)掩蓋鋅對(duì)微生物的影響。(3)濃度從0.1mg/L到1.6mg/L的土霉素對(duì)微生物呼吸的影響不大,總體表現(xiàn)先抑制后恢復(fù)。隨著時(shí)間的推移可以消除土霉素對(duì)微生物呼吸的影響。濃度從0.05mg/L到0.8mg/L的磺胺間甲氧嘧啶對(duì)微生物呼吸的影響總體表現(xiàn)先抑制再促進(jìn)后恢復(fù)。濃度從0.1mg/L到1.6mg/L的土霉素、濃度從0.05mg/L到0.8mg/L的磺胺間甲氧嘧啶都會(huì)刺激微生物的代謝,不會(huì)出現(xiàn)像濃度16mg/L銅、濃度32mg/L鋅那樣對(duì)微生物代謝的嚴(yán)重抑制作用。(4)土霉素和磺胺間甲氧嘧啶復(fù)合時(shí),對(duì)微生物呼吸影響表現(xiàn)為先抑制再促進(jìn)后恢復(fù)。土霉素、磺胺間甲氧嘧啶對(duì)ETS活性抑制率的影響都不顯著。土霉素和磺胺間甲氧嘧啶之間交互作用明顯。(5)本研究范圍濃度的銅比實(shí)驗(yàn)范圍濃度的土霉素的毒性更大。銅與土霉素聯(lián)合對(duì)ETS活性的影響與銅濃度密切相關(guān)。土霉素的聯(lián)合可以提高銅對(duì)ETS活性的抑制率。單因素銅對(duì)微生物代謝的抑制濃度為16mg/L,土霉素的聯(lián)合可以把銅對(duì)微生物代謝的抑制濃度降低至8mg/L。
[Abstract]:The problem of water pollution is becoming more and more serious. Among all the pollution of water environment, the pollution caused by animal husbandry can not be ignored, especially the pollution of pig wastewater. Heavy metals and antibiotics are widely used in pig industry. A large number of heavy metals and antibiotics followed the waste water into the wastewater treatment system, which added a lot of difficulties for the subsequent wastewater treatment. The purpose of this study was to investigate the effects of heavy metals and antibiotics on microorganisms in SBBR reactor in wastewater treatment system, and to lay a theoretical foundation for wastewater treatment in pig farms. The effects of different concentrations of heavy metals such as copper and zinc and antibiotics such as oxytetracycline and sulfamethoxine on microbes were investigated in a normal SBBR reactor. The results are as follows: (1) when copper concentration is less than 2 mg / L, copper can promote microbial respiration, and the promotion effect of 2 mg / L copper concentration is greater than that of 1 mg / L copper concentration and 0.5 mg / L copper concentration. When copper concentration is greater than 4 mg / L, copper can inhibit microbial respiration, and the inhibitory effect of 16 mg / L copper is greater than that of 4 mg / L copper and 8 mg / L copper. When zinc concentration is less than 4 mg / L, zinc can promote microbial respiration, and the effect of 4 mg / L zinc concentration is greater than that of 2 mg / L zinc concentration and 1 mg / L zinc concentration. When zinc concentration is greater than 8 mg / L, zinc can inhibit microbial respiration, and the inhibition of 32 mg / L zinc is greater than that of 16 mg / L zinc and 8 mg / L zinc. (2) when copper concentration is less than 8 mg / L, it stimulates microbial metabolism. Only copper concentrations greater than 16 mg / L inhibit microbial metabolism. When zinc concentration is less than 16 mg / L, it stimulates microbial metabolism, and only when zinc concentration is higher than 32 mg / L can inhibit microbial metabolism. The results show that the effect of copper on microbial metabolism is stronger than that of zinc on microbial metabolism. Copper and zinc compound showed synergistic inhibition on microbial respiration and metabolism. The combination of copper and zinc increases the toxicity of single factor copper and zinc to microorganisms. (3) the concentration of oxytetracycline from 0.1 mg / L to 1.6 mg / L had little effect on microbial respiration. The effect of oxytetracycline on microbial respiration can be eliminated over time. The effects of sulfamethoxine on microbial respiration from 0.05 mg / L to 0.8 mg / L showed inhibition first and then promotion and then recovery. Concentrations from 0.1 mg / L to 1.6 mg / L of oxytetracycline, from 0.05 mg / L to 0.8 mg / L of sulfamethoxine, all stimulate microbial metabolism, without the appearance of concentrations of 16 mg / L copper. (4) when oxytetracycline and sulfamethoxine were combined, the effect on microbial respiration was inhibited first, then promoted and then recovered. Oxytetracycline and sulfamethoxine had no significant effect on the inhibition rate of ETS activity. The interaction between oxytetracycline and sulfamethoxine was obvious. (5) the copper concentration in this study range was more toxic than that in the experimental range. The effect of copper combined with oxytetracycline on ETS activity is closely related to copper concentration. The combination of oxytetracycline can increase the inhibition rate of copper on ETS activity. The inhibitory concentration of single factor copper on microbial metabolism was 16 mg / L, and the inhibition concentration of copper on microbial metabolism was reduced to 8 mg / L with oxytetracycline.
【學(xué)位授予單位】:南昌大學(xué)
【學(xué)位級(jí)別】:碩士
【學(xué)位授予年份】:2015
【分類(lèi)號(hào)】:X713;X172

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 向彬;王彬;徐衛(wèi)紅;熊治庭;;重金屬Cd、Zn、Cu、Pb對(duì)土壤微生物和酶活性的影響[J];氨基酸和生物資源;2012年03期

2 韓桂琪;王彬;徐衛(wèi)紅;陳貴青;王慧先;張海波;張曉t,

本文編號(hào):2096846


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