本文選題：酸性森林土 + 氨氧化古菌(AOA)��； 參考：《應用生態(tài)學報》2017年05期

【摘要】：以小興安嶺酸性森林泥炭土為研究對象,通過加入10 mL·L~(-1)乙炔及不同濃度的外源硫酸銨(0、1.2、6.0 mmol N·kg~(-1)進行硝化培養(yǎng)試驗,探究酸性泥炭土中硝化作用類型及主要驅(qū)動因子.結(jié)果表明:無論有無外加氮源,酸性泥炭土均存在較強的礦化作用(0.9~1.4mg N·kg~(-1)·d~(-1),經(jīng)過2周的培養(yǎng)均發(fā)生了硝化作用(0.4~0.6 mgN·kg~(-1)·d~(-1)),且不同濃度硫酸銨處理之間無顯著差異;而乙炔處理雖有較強的礦化作用(0.8 mg N·kg~(-1)·d~(-1)),但未發(fā)生明顯的硝化作用(0 mg N·kg~(-1)·d~(-1)),說明該酸性泥炭土以自養(yǎng)硝化為主,外源無機氮源濃度對硝化作用無顯著影響,硝化底物NH_3的主要來源不是外源硫酸銨,更可能來源于土壤中有機氮的礦化.培養(yǎng)0~14 d,無論有無外加氮源,酸性泥炭土氨氧化細菌(AOB)和古菌(AOA)豐度均顯著增加,但不同濃度硫酸銨處理間無顯著差異,表明外源無機氮濃度對氨氧化微生物的生長無顯著促進作用.與不加乙炔的對照相比,乙炔處理AOB和AOA豐度隨時間均無顯著變化,推測AOA與AOB在該酸性泥炭土的硝化過程中都可能起一定的作用.
[Abstract]:The nitrification types and main driving factors of acid forest peat soil in Xiaoxinganling were studied by adding 10 mL L ~ (-1) acetylene and different concentrations of exogenous ammonium sulfate (0 ~ 1.22 ~ (6) mmol N kg ~ (-1). The results showed that there was strong mineralization (0.9~1.4mg N kg-1 d ~ (-1) in acidic peat with or without nitrogen source, and nitrification (0.4 ~ 0. 6 mg N ~ (-1) d ~ (-1),) occurred after 2 weeks culture, and there was no significant difference among different concentrations of ammonium sulfate. Although acetylene treatment had strong mineralization (0.8 mg N kg-1 d ~ (-1), there was no obvious nitrification (0 mg N kg ~ (-1) d-1), indicated that autotrophic nitrification was the main factor of nitrification in the acidic peat soil, and the concentration of exogenous inorganic nitrogen source had no significant effect on nitrification. The main source of NH3 is not exogenous ammonium sulfate, but more likely from the mineralization of organic nitrogen in soil. The abundance of ammonia-oxidizing bacteria (AOB) and actinomycetes (AOA) in acidic peat soil increased significantly with or without additional nitrogen sources for 14 days, but there was no significant difference between different concentrations of ammonium sulfate treatment. The results showed that the concentration of inorganic nitrogen had no significant effect on the growth of ammonia-oxidized microorganisms. Compared with the control without acetylene, the AOB and AOA abundance of acetylene treatment did not change significantly with time, which suggested that both AOA and AOB might play a certain role in the nitrification process of the acid peat soil.
【作者單位】： 西南大學資源環(huán)境學院;
【基金】：國家自然科學基金項目(41671232,41271267)資助~~
【分類號】：S714

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