本文選題：酸性森林土 + 氨氧化古菌(AOA)��； 參考：《應(yīng)用生態(tài)學(xué)報(bào)》2017年05期

【摘要】：以小興安嶺酸性森林泥炭土為研究對(duì)象,通過(guò)加入10 mL·L~(-1)乙炔及不同濃度的外源硫酸銨(0、1.2、6.0 mmol N·kg~(-1)進(jìn)行硝化培養(yǎng)試驗(yàn),探究酸性泥炭土中硝化作用類(lèi)型及主要驅(qū)動(dòng)因子.結(jié)果表明:無(wú)論有無(wú)外加氮源,酸性泥炭土均存在較強(qiáng)的礦化作用(0.9~1.4mg N·kg~(-1)·d~(-1),經(jīng)過(guò)2周的培養(yǎng)均發(fā)生了硝化作用(0.4~0.6 mgN·kg~(-1)·d~(-1)),且不同濃度硫酸銨處理之間無(wú)顯著差異;而乙炔處理雖有較強(qiáng)的礦化作用(0.8 mg N·kg~(-1)·d~(-1)),但未發(fā)生明顯的硝化作用(0 mg N·kg~(-1)·d~(-1)),說(shuō)明該酸性泥炭土以自養(yǎng)硝化為主,外源無(wú)機(jī)氮源濃度對(duì)硝化作用無(wú)顯著影響,硝化底物NH_3的主要來(lái)源不是外源硫酸銨,更可能來(lái)源于土壤中有機(jī)氮的礦化.培養(yǎng)0~14 d,無(wú)論有無(wú)外加氮源,酸性泥炭土氨氧化細(xì)菌(AOB)和古菌(AOA)豐度均顯著增加,但不同濃度硫酸銨處理間無(wú)顯著差異,表明外源無(wú)機(jī)氮濃度對(duì)氨氧化微生物的生長(zhǎng)無(wú)顯著促進(jìn)作用.與不加乙炔的對(duì)照相比,乙炔處理AOB和AOA豐度隨時(shí)間均無(wú)顯著變化,推測(cè)AOA與AOB在該酸性泥炭土的硝化過(guò)程中都可能起一定的作用.
[Abstract]:The nitrification types and main driving factors of acid forest peat soil in Xiaoxinganling were studied by adding 10 mL L ~ (-1) acetylene and different concentrations of exogenous ammonium sulfate (0 ~ 1.22 ~ (6) mmol N kg ~ (-1). The results showed that there was strong mineralization (0.9~1.4mg N kg-1 d ~ (-1) in acidic peat with or without nitrogen source, and nitrification (0.4 ~ 0. 6 mg N ~ (-1) d ~ (-1),) occurred after 2 weeks culture, and there was no significant difference among different concentrations of ammonium sulfate. Although acetylene treatment had strong mineralization (0.8 mg N kg-1 d ~ (-1), there was no obvious nitrification (0 mg N kg ~ (-1) d-1), indicated that autotrophic nitrification was the main factor of nitrification in the acidic peat soil, and the concentration of exogenous inorganic nitrogen source had no significant effect on nitrification. The main source of NH3 is not exogenous ammonium sulfate, but more likely from the mineralization of organic nitrogen in soil. The abundance of ammonia-oxidizing bacteria (AOB) and actinomycetes (AOA) in acidic peat soil increased significantly with or without additional nitrogen sources for 14 days, but there was no significant difference between different concentrations of ammonium sulfate treatment. The results showed that the concentration of inorganic nitrogen had no significant effect on the growth of ammonia-oxidized microorganisms. Compared with the control without acetylene, the AOB and AOA abundance of acetylene treatment did not change significantly with time, which suggested that both AOA and AOB might play a certain role in the nitrification process of the acid peat soil.
【作者單位】： 西南大學(xué)資源環(huán)境學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(41671232,41271267)資助~~
【分類(lèi)號(hào)】：S714

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