深紅蟲草高產(chǎn)卵孢菌素的培養(yǎng)基篩選及發(fā)酵條件優(yōu)化
發(fā)布時(shí)間:2018-06-26 01:00
本文選題:深紅蟲草 + 卵孢菌素 ; 參考:《菌物學(xué)報(bào)》2017年04期
【摘要】:本研究以提高深紅蟲草Cordyceps cardinalis C033次級(jí)代謝產(chǎn)物卵孢菌素的產(chǎn)量為目標(biāo),對(duì)其產(chǎn)卵孢菌素的培養(yǎng)基進(jìn)行篩選,并利用單因素和正交試驗(yàn)對(duì)深紅蟲草產(chǎn)卵孢菌素的發(fā)酵條件進(jìn)行了優(yōu)化。結(jié)果表明,深紅蟲草產(chǎn)卵孢菌素的最適發(fā)酵培養(yǎng)基為馬鈴薯 蛋白胨 葡萄糖培養(yǎng)基,最佳發(fā)酵參數(shù)為:發(fā)酵時(shí)間6d,培養(yǎng)基初始p H 7.0,接種量6%,培養(yǎng)溫度24℃,每500m L錐形瓶裝液量100m L,搖瓶轉(zhuǎn)速120r/min。用最佳培養(yǎng)基進(jìn)行發(fā)酵條件優(yōu)化后獲得卵孢菌素448.70μg/m L,產(chǎn)量是優(yōu)化前的10.14倍。該研究結(jié)果為菌株C033在農(nóng)業(yè)害蟲防治上的應(yīng)用奠定了基礎(chǔ)。
[Abstract]:The aim of this study was to improve the yield of the secondary metabolite of Cordyceps cardinalis C033, and to screen the culture medium for ovulin, a secondary metabolite of Cordyceps cardinalis C033. The fermentation conditions of oosporin from Cordyceps sinensis were optimized by single factor and orthogonal test. The results showed that the optimum fermentation medium for oosporin of Cordyceps sinensis was potato peptone and glucose medium. The optimum fermentation parameters were as follows: fermentation time 6 days, initial pH 7.0, inoculation amount 6, culture temperature 24 鈩,
本文編號(hào):2068326
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