發(fā)布時間：2018-06-19 22:37

  本文選題：紅壤稻田 + 硝化作用��； 參考：《中國土壤與肥料》2017年01期

【摘要】：為了解南方紅壤稻田參與土壤氮循環(huán)過程的功能微生物及其與環(huán)境因子間的普遍規(guī)律性關系,應用熒光定量PCR方法研究了稻田中不同時期氨氧化古菌氨單加氧酶編碼基因AOA-amoA、硝酸鹽還原酶編碼基因narG和N_2O還原酶編碼基因nosZ基因豐度的變化,結(jié)合主成分分析(PCA)、冗余分析(RDA)探討了有關硝化、反硝化微生物功能基因豐度變化及其與土壤環(huán)境因子間的相互關系。結(jié)果表明:AOA-amoA、narG和nosZ基因豐度間極顯著正相關;AOA-amo A、narG和nosZ基因豐度隨水稻生育進程逐漸降低,在早稻成熟期顯著低于翻地前及早稻移栽前。土壤有機質(zhì)、電導率、微生物生物量碳、微生物生物量氮、pH值、Eh及鹽酸提取態(tài)Fe(Ⅱ)對AOA-amoA、narG和nosZ基因豐度的變化有重要影響,其中鹽酸提取態(tài)Fe(Ⅱ)與其相關性最強。本研究探討了紅壤稻田氮轉(zhuǎn)化微生物在不同時期的數(shù)量變化及其環(huán)境影響因素,為合理調(diào)控紅壤稻田氮轉(zhuǎn)化提供了理論依據(jù)。
[Abstract]:In order to understand the functional microbes involved in soil nitrogen cycling in red soil paddy fields in southern China and their general regular relationship with environmental factors. Fluorescence quantitative PCR (FQ-PCR) was used to study the changes of AOA-amo A, narg and NosZ gene abundance of ammonia monooxygenase coding gene, nitrate reductase gene narg and N2O reductase gene in different stages of ammonia-oxidizing palaeomycetes in paddy fields. Combined with principal component analysis (PCAA) and redundancy analysis (RDA), the changes of microbial functional gene abundance of nitrification and denitrification and their relationship with soil environmental factors were discussed. The results showed that the abundance of AOA-amo Aannar G and NosZ genes decreased gradually with the growth of rice, and were significantly lower at maturity stage of early rice than before turning over and transplanting early rice. The results showed that the abundance of AOA-amo Aannar G and NosZ gene was significantly positive correlation between the two genes. Soil organic matter, electrical conductivity, microbial biomass carbon, microbial biomass nitrogen pH value (Eh) and hydrochloric acid extracted Fe (鈪，

本文編號：2041624