本文選題：土壤微生物多樣性 + 土壤有效養(yǎng)分�。� 參考：《生態(tài)學報》2016年18期

【摘要】：采用滅菌土壤分別接種不同稀釋倍數(1、10~(-2)、10~(-4)和10~(-6))未滅菌土壤懸濁液的方法,研究了土壤微生物多樣性降低對油菜生長和養(yǎng)分吸收、土壤養(yǎng)分有效性和酶活性的影響。結果表明:(1)隨著接種土壤懸濁液稀釋倍數增加,油菜生物量逐漸降低,10-4的油菜生物量顯著低于1和10~(-2),10~(-6)僅為1的26%;(2)油菜氮、磷和鉀的吸收量與油菜生物量呈現(xiàn)相同的變化規(guī)律;(3)土壤銨態(tài)氮濃度隨接種土壤懸濁液稀釋倍數增加而降低;而土壤硝態(tài)氮則以10~(-4)為最高,其它處理間沒有顯著差異;土壤有效磷未發(fā)生顯著變化;有效鉀反而有上升趨勢;(4)土壤多酚氧化酶(PhO X)活性隨接種土壤懸濁液稀釋倍數增加逐漸升高;β-1,4-葡萄糖苷酶(βG)活性以10~(-6)為最高,而其它處理差異不顯著;土壤亮氨酸酶氨肽酶(LAP)活性和酸性磷酸酶(AP)活性變化不顯著;(5)相關分析表明,油菜生物量與土壤銨態(tài)氮濃度的對數顯著正相關;與多酚氧化酶、葡萄糖苷酶和亮氨酸氨肽酶活性顯著負相關。研究表明,微生物多樣性降低主要通過抑制土壤氮素釋放影響植物生長。
[Abstract]:The effects of soil microbial diversity on rapeseed growth, nutrient uptake, soil nutrient availability and enzyme activity were studied by inoculating different dilutions of different dilutions (1 ~ 10 ~ 10 ~ (-10) ~ (-2) and 10 ~ (10) ~ (-6) ~ (-1) of unsterilized soil suspensions. The results showed that with the increase of dilution ratio of suspensions in inoculated soil, the biomass of rapeseed decreased gradually. The biomass of Brassica napus 10 ~ (-4) was significantly lower than that of 1 and 10 ~ 10 ~ (2) C ~ (-1). The biomass of rape was only 26 ~ (2) N of 1). The uptake of phosphorus and potassium was the same as that of rape biomass. (3) the concentration of soil ammonium nitrogen decreased with the increase of dilution times of suspensions in inoculated soil, but the nitrate nitrogen in soil was the highest in 10 ~ (10) ~ (-4), but there was no significant difference between other treatments. There was no significant change in available phosphorus in soil, but there was an increasing trend in available potassium. The activity of polyphenol oxidase (PPO) in soil gradually increased with the dilution multiple of soil suspensions, and the activity of 尾 -1 + 4-glucosidase (尾 -G6) was the highest. The results of correlation analysis showed that the biomass of rape was positively correlated with the logarithm of soil ammonium nitrogen concentration, and with polyphenol oxidase. The activities of glucosidase and leucine aminopeptidase were negatively correlated. The results showed that the decrease of microbial diversity affected plant growth mainly by inhibiting soil nitrogen release.
【作者單位】： 中國農業(yè)科學院農業(yè)資源與農業(yè)區(qū)劃研究所農業(yè)部植物營養(yǎng)與肥料重點實驗室;中國農業(yè)科學院農業(yè)環(huán)境與可持續(xù)發(fā)展研究所;浙江大學教育部環(huán)境修復與生態(tài)健康教育部重點實驗室;

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