本文選題：九龍山自然保護區(qū) + 林麝棲息地��； 參考：《西北林學院學報》2017年02期

【摘要】：為了解四川九龍山自然保護區(qū)林麝棲息地典型植被(灌木林、針闊混交林和闊葉混交林)下土壤微生物群落特征,探討土壤微生物類群對地表植被組成的影響。采用平板稀釋法進行活菌計數(shù)與菌株分離,16SrRNA PCR鑒定分離菌,常規(guī)方法測定土壤理化學性質(zhì)。結(jié)果表明,棲息地各植被類型下共由11種細菌菌屬組成;以芽孢菌占主要部分,韋氏芽胞桿菌(Bacillus weihenstephanensis)、巨大芽胞桿菌(Bacillus megaterium)和蘇云金芽胞桿菌(Bacillus thuringiensis)為優(yōu)勢菌群。各植被類型土壤均以細菌為主,放線菌次之,真菌最少;土壤微生物總數(shù)排序為灌木林針闊混交林闊葉混交林;各植被類型下不同土層中微生物的分布趨勢是0~20cm20~40cm40~60cm。相關(guān)性分析結(jié)果表明,pH值與放線菌呈顯著正相關(guān)性(R=0.676,P0.05),總微生物與細菌總數(shù)呈極顯著正相關(guān)性(R=1,P0.01),總微生物與放線菌呈顯著正相關(guān)性(R=1,P0.01)。
[Abstract]:In order to understand the characteristics of soil microbial community under typical vegetation (shrub, coniferous and broad-leaved mixed forest) in forest and musk deer habitat in Jiulong Mountain Nature Reserve, Sichuan Province, the effects of soil microbial groups on the composition of surface vegetation were studied. The isolated bacteria were identified by plate dilution method and 16s rRNA PCR, and the soil physiochemical properties were determined by conventional method. The results showed that the habitat was composed of 11 species of bacteria, with Bacillus Weihenstephanensis, Bacillus megateriumum and Bacillus thuringiensis as dominant groups. The soil was dominated by bacteria, followed by actinomycetes, and fungi were the least. The total number of soil microbes was ranked as broad-leaved mixed forest of shrub forest and broad-leaved mixed forest, and the distribution trend of microbes in different soil layers under different vegetation types was 0 ~ 20 cm ~ 20 ~ 40 cm ~ 40 cm ~ (40) cm ~ (60 cm). The results of correlation analysis showed that there was a significant positive correlation between pH value and actinomycetes, a significant positive correlation between total microorganisms and the total number of bacteria, and a significant positive correlation between total microbes and actinomycetes, and a significant positive correlation between total microbes and actinomycetes.
【作者單位】： 四川養(yǎng)麝研究所;四川九龍山自然保護區(qū);四川農(nóng)業(yè)大學都江堰校區(qū)微生物學實驗室;
【基金】：四川省林業(yè)廳省級自然保護區(qū)重點物種科學研究項目 四川農(nóng)業(yè)大學雙支項目
【分類號】：S714.3

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