本文選題：長期定位施肥 + 石灰性紫色水稻土��； 參考：《生態(tài)學(xué)報》2016年21期

【摘要】：研究不同施肥制度對水稻土氨氧化古菌(AOA)群落結(jié)構(gòu)和垂直分布特征的影響,可以深入認(rèn)識不同施肥制度下的石灰性紫色水稻土氮素循環(huán)特征及微生物驅(qū)動機(jī)制,為該地區(qū)科學(xué)施肥、培肥地力提供理論依據(jù)。利用化學(xué)分析和變性梯度凝膠電泳(DGGE)對不同施肥制度下石灰性紫色水稻土理化性質(zhì)和AOA群落結(jié)構(gòu)進(jìn)行了分析。結(jié)果顯示:相對于無肥處理,施肥會降低石灰性紫色水稻土pH和硝氮含量,而增加土壤有機(jī)質(zhì)、全氮和氨氮含量。伴隨土壤深度增加,土壤pH增加,全氮和硝氮含量降低,氨氮含量變化趨勢不明顯。不同施肥制度在不同土壤深度對石灰性紫色水稻土AOA群落結(jié)構(gòu)產(chǎn)生不同的脅迫效應(yīng),不同施肥制度下的AOA群落結(jié)構(gòu)在0—20 cm處差異不明顯;土壤深度增加,不同施肥制度下的AOA群落結(jié)構(gòu)表現(xiàn)出明顯差異,CK和N肥處理下的AOA群落結(jié)構(gòu)較簡單。AOA群落結(jié)構(gòu)多樣性指數(shù)和豐富度隨土壤深度增加而減小。石灰性紫色水稻土AOA與來自不同土壤和水體環(huán)境的AOA具有明顯相似性。冗余梯度分析(RDA)顯示pH(P=0.012)是造成石灰性紫色水稻土AOA群落結(jié)構(gòu)差異的主要原因。研究揭示石灰性紫色水稻土中的AOA群落結(jié)構(gòu)受施肥制度明顯影響并表現(xiàn)出明顯的垂直分布特征。
[Abstract]:The effects of different fertilization systems on the community structure and vertical distribution of AOAA in paddy soils were studied. The nitrogen cycling characteristics and microbial driving mechanism of calcareous purple paddy soils under different fertilization systems were studied. It provides a theoretical basis for scientific fertilization and fertility in this area. The physicochemical properties and AOA community structure of calcareous purple paddy soils under different fertilization systems were analyzed by chemical analysis and denaturing gradient gel electrophoresis (DGGE). The results showed that fertilization decreased pH and nitrate content of calcareous purple paddy soil, but increased soil organic matter, total nitrogen and ammonia nitrogen content. With the increase of soil depth, soil pH increased, total nitrogen and nitrate content decreased, but the change trend of ammonia nitrogen content was not obvious. Different fertilization systems had different stress effects on AOA community structure of calcareous purple paddy soil under different soil depths. The AOA community structure under different fertilization systems had no significant difference at 0-20 cm, and the soil depth increased. The community structure of AOA under different fertilization systems showed obvious difference. The diversity index and richness of AOA community under CK and N fertilizer treatments decreased with the increase of soil depth. The AOA of calcareous purple paddy soil was similar to that of AOA from different soil and water environment. Redundant gradient analysis (RDAA) showed that pH (P0. 012) was the main reason for the difference of AOA community structure in calcareous purple paddy soils. The results showed that the community structure of AOA in calcareous purple paddy soil was obviously affected by fertilization system and showed obvious vertical distribution.
【作者單位】： 四川農(nóng)業(yè)大學(xué)資源學(xué)院微生物系;四川省農(nóng)業(yè)科學(xué)院土壤肥料研究所;
【基金】：國家自然科學(xué)基金項目(41201256) 四川省自然科學(xué)基金項目(SC200902158) 四川農(nóng)業(yè)大學(xué)“雙支計劃”項目(01470702)
【分類號】：S154.3

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本文編號：1990655