本文選題：馬拉硫磷 + 微生物降解��； 參考：《河南農(nóng)業(yè)科學(xué)》2017年09期

【摘要】：為了利用微生物降解土壤中殘留的有機(jī)磷農(nóng)藥,采用磷鉬藍(lán)比色法測(cè)定了菌株MN-13降解馬拉硫磷及其他有機(jī)磷農(nóng)藥的性能,并通過(guò)單因素選擇、正交優(yōu)化試驗(yàn)對(duì)試驗(yàn)菌株的生長(zhǎng)培養(yǎng)基進(jìn)行了優(yōu)化。結(jié)果表明,試驗(yàn)菌株MN-13對(duì)25%馬拉硫磷、40%氧化樂(lè)果、50%乙基對(duì)硫磷的降解率分別達(dá)到91.52%、69.24%、49.68%。培養(yǎng)基優(yōu)化中,通過(guò)單因素試驗(yàn)選擇的碳源、氮源、NaCl及pH值的中心點(diǎn)分別為葡萄糖1.5%、牛肉膏1.0%、NaCl 0.5%、pH值8.0;以此為基礎(chǔ)進(jìn)行正交試驗(yàn),優(yōu)化后培養(yǎng)基組成為葡萄糖1.5%、牛肉膏1.2%、NaCl 0.6%、pH值8.5,菌體OD_(600)比優(yōu)化前提高了33.1%。綜上,MN-13菌株可有效降解馬拉硫磷等有機(jī)磷農(nóng)藥,其在優(yōu)化后的培養(yǎng)基中生長(zhǎng)量有所提高。
[Abstract]:In order to degrade residual organophosphorus pesticides in soil by microorganism, the degradation properties of malathion and other organophosphorus pesticides by strain MN-13 were determined by molybdenum blue colorimetry, and single factor selection was used to determine the degradation of malathion and other organophosphorus pesticides. The growth medium of the tested strain was optimized by orthogonal optimization test. The results showed that the degradation rates of 25% malathion and 40% omethoate and 50% ethylparathion reached 91.52% 69.24% and 49.68%, respectively. In the optimization of culture medium, the carbon source selected by single factor test, the center point of nitrogen source NaCl and pH value were glucose 1.5 and 1.0% NaCl 0.5, respectively, and the pH value was 8.0, which was used as the basis of orthogonal experiment. The optimized medium consisted of glucose 1.5, beef paste 1.2% NaCl 0.6 and pH 8.5, and the biomass ODS600 increased 33.1% compared with those before optimization. The strain MN-13 could effectively degrade malathion and other organophosphorus pesticides, and its growth in the optimized medium was improved.
【作者單位】： 延安大學(xué);
【基金】：國(guó)家大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃項(xiàng)目(201510719287) 陜西省大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃項(xiàng)目(1447)
【分類(lèi)號(hào)】：X172;X592

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