發(fā)布時間：2018-06-03 11:17

  本文選題：芝麻餅肥 + 芝麻素　； 參考：《河南工業(yè)大學學報(自然科學版)》2017年03期

【摘要】：利用高效液相色譜法同時測定芝麻餅肥中芝麻素和芝麻林素的含量。液相色譜條件為:流動相為甲醇水溶液(V_(甲醇)∶V_水=80∶20),流速1.0 m L/min,進樣量5.0μL,柱溫為室溫,紫外檢測器波長287 nm。檢驗結果表明:被測兩峰完全分離,且峰形較好,芝麻素和芝麻林素均在1~100 mg/L范圍內線性關系良好,R~2分別為1.000 0、0.999 9;該方法的精密度、重現(xiàn)性及準確度均較好,芝麻素平均回收率在98.2%~100.2%之間,芝麻林素平均回收率在96.7%~99.8%之間;樣品提取液在2 h內穩(wěn)定。芝麻餅肥中芝麻素和芝麻林素總含量為1.2~133.7 mg/100 g,芝麻素與芝麻林素含量比例為0.9~3.1。試驗驗證了高效液相色譜法同時測定芝麻餅肥中芝麻素、芝麻林素含量的可行性,為芝麻餅肥的質量檢測提供了技術支持。
[Abstract]:The content of sesamin and sesamin in sesame cake fertilizer was determined by HPLC. The liquid chromatography conditions are as follows: the mobile phase is methanol aqueous solution (methanol: v _ water 80: 20), the flow rate is 1.0 mL / min, the injection amount is 5.0 渭 L, the column temperature is room temperature, the UV detector wavelength is 287 nm. The results showed that the two peaks were completely separated, and the peak shape was better. The linear relationship between sesamin and sesamin was 1.000 ~ 0.999 9 in the range of 1 ~ 100 mg/L. The precision, reproducibility and accuracy of the method were good. The average recoveries of sesamin and sesamin were between 98.2% and 100.2%, respectively, and the average recoveries of sesamin and sesamin were 96.7% and 99.8%, respectively, and the sample extract was stable within 2 hours. The total content of sesamin and sesamin in sesame cake fertilizer was 1.2133.7 mg/100 / g, and the ratio of sesamin to sesamin was 0.9g / 3.1.The total content of sesamin and sesamin in sesame cake fertilizer was 1.2133.7 mg/100 / g. The feasibility of simultaneous determination of sesamin and sesamin in sesame cake fertilizer by HPLC was verified, which provided technical support for the quality detection of sesame cake fertilizer.
【作者單位】： 河南省產品質量監(jiān)督檢驗院;
【基金】：河南省科技計劃項目(2016ZJ07)
【分類號】：O657.72;TQ440.72

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本文編號：1972592