本文選題：木薯細(xì)菌性枯萎病 + 分離和篩選��； 參考：《熱帶作物學(xué)報(bào)》2017年08期

【摘要】：從木薯8個(gè)不同生境分離到298株細(xì)菌,測定其產(chǎn)酶、次生代謝物和頡頏活性,結(jié)果表明,產(chǎn)蛋白酶、纖維素酶、葡聚糖酶和生長素的菌株和比例分別為160(53.69%)、57(19.13%)、11(3.69%)和150(50.34%),產(chǎn)嗜鐵素菌株最多203個(gè),比例達(dá)68.12%;產(chǎn)幾丁質(zhì)酶的菌株最少7株,比例僅為2.35%;其中,9株菌株對木薯細(xì)菌性枯萎病菌具有頡頏活性,比例為3.02%。通過賦值選擇88個(gè)得分較高的菌株,對其16S r RNA測序及ARDRA-PCR和BOX-PCR聚類分析,發(fā)現(xiàn)可分成21個(gè)簇,且每個(gè)簇的種群豐度較高。優(yōu)選11個(gè)菌株進(jìn)行日光溫室大棚盆栽防效測定,其中,HWY-3-1防效為100%,防效在60%以上的菌株有DBS-5(85.96%)、HS-4-3(82.88%)、DHWP-1(79.37%)、HWYT-3-2(77.88%)、HWS-4-3(77.84%)、DHNS-3-5(65.34%)、DHWR-5-1(61.83%),和對照防效相比,差異顯著;進(jìn)一步選擇5個(gè)防效較好的潛力菌株測定其田間防效,結(jié)果顯示,HWY-3-1(Bacillus pumilus)平均防效為38.11%,與對照的防效存在顯著差異。試驗(yàn)篩選策略可行,初步找到1株對CBB防效較穩(wěn)定的生防菌株Bacillus pumilus。
[Abstract]:298 strains of bacteria were isolated from 8 different habitats of cassava, and their enzyme production, secondary metabolites and antagonistic activities were determined. The results showed that protease and cellulase were produced. The strains and ratios of glucanase and auxin were 160 (53.69g) and 150 (50.34), respectively. Most 203 strains produced ferritin, the proportion was 68.122.The strains producing chitinase were at least 7 strains, the proportion was only 2.35; among them, 9 strains had antagonistic activity against cassava bacterial wilt. The ratio is 3.02. The 16s r RNA sequencing and ARDRA-PCR and BOX-PCR cluster analysis showed that there were 21 clusters, and the population abundance of each cluster was higher. Eleven strains were selected for pot experiment in solar greenhouse. The control effect of HWY-3-1 was 100, and the control effect was more than 60%. There were more than 60% strains with DBS-5HS-4-85.96 and DHWP-1 79.37370.The field control effect of DHWP-1 was 77.884-77.884-77.845.84 DHNS-3-565.344DHWR-51.8330.The field control effect of DHWY-3-1 was higher than that of the control, and the field control effect of DHWY-3-1 DHWR-51.8333A was significantly different from that of the control, and the field control effect of DHWY-3-1 was significantly different from that of the control, and the control effect of DHWY-3-1 was higher than that of the control, and the control effect of DHWY-3-1 was significantly higher than that of the control. The results showed that the average control effect of HWY-3-1 Bacillus pumilus was 38.11, and there was significant difference between HWY-3-1 Bacillus pumilus and control. The screening strategy was feasible and a stable biocontrol strain Bacillus pumilus was found.
【作者單位】： 中國熱帶農(nóng)業(yè)科學(xué)院廣州實(shí)驗(yàn)站;華南農(nóng)業(yè)大學(xué)農(nóng)學(xué)院;海南大學(xué)熱帶農(nóng)林學(xué)院;華南農(nóng)業(yè)大學(xué)材料與能源學(xué)院;
【基金】：海南省自然科學(xué)基金項(xiàng)目“芽孢桿菌對木薯細(xì)菌性枯萎病防效及葉圍微生態(tài)影響研究”(No.20153128) 熱科院生物所中央級(jí)公益性科研院所基本科研業(yè)務(wù)費(fèi)專項(xiàng)“木薯細(xì)菌性枯萎病生防菌株篩選及機(jī)理研究”(No.ITBB140306) 現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系建設(shè)專項(xiàng)資金(No.CARS-12)
【分類號(hào)】：S435.33;S476.1
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本文編號(hào)：1944977