本文選題：鹽堿脅迫 + 黃瓜嫁接��； 參考：《中國生態(tài)農(nóng)業(yè)學(xué)報》2017年11期

【摘要】：為了解鹽堿脅迫對黃瓜嫁接苗根際土壤細(xì)菌和真菌群落結(jié)構(gòu)的影響,本研究以2種耐鹽堿砧木‘華砧108’(T1)、‘神力鐵木砧’(T2)和2種鹽堿敏感砧木‘輝太郎’(S1)、‘京欣砧6號’(S2)為試材,自根苗作為對照,以混合鹽(鹽分摩爾比為NaHCO_3∶Na_2SO_4∶NaCl∶Na_2CO_3=4∶2∶2∶0.15)濃度為100 mmol·L~(-1)、pH 9.0的處理液處理20 d、30 d、40 d(定植30 d、40 d、50 d),利用PCR-DGGE技術(shù),研究了鹽堿脅迫對不同砧木嫁接的黃瓜幼苗根際土壤微生物群落結(jié)構(gòu)和豐度的影響。結(jié)果表明,耐鹽堿的砧木品種T1、T2根際土壤真菌DGGE圖譜條帶數(shù)顯著高于鹽堿敏感的S2和自根苗對照CK,并且耐鹽堿的品種T2土壤細(xì)菌的Shannon-Wiener指數(shù)與均勻度指數(shù)均顯著高于鹽堿敏感的品種S1、S2和自根苗對照CK。耐鹽堿品種T1的細(xì)菌16S rDNA基因拷貝數(shù)在定植50 d時顯著高于鹽堿敏感的品種及自根苗;在定植40 d時,耐鹽堿的砧木T2真菌ITS基因拷貝數(shù)顯著高于鹽堿敏感的品種以及黃瓜自根苗;定植50 d時,耐鹽堿的砧木真菌ITS基因拷貝數(shù)顯著高于鹽堿敏感的品種,但與自根苗差異不顯著。不同耐鹽堿性砧木嫁接黃瓜幼苗根際土壤微生物群落結(jié)構(gòu)組成和豐度存在差異。以上研究表明,隨著鹽堿脅迫時間的增加,耐鹽堿性不同的砧木嫁接苗根際土壤微生物群落豐度與結(jié)構(gòu)多樣性產(chǎn)生了較大差異,間接改變了土壤微生態(tài)環(huán)境,致使土壤微生物數(shù)量和豐富度也發(fā)生改變。耐鹽堿的砧木品種可能通過改善土壤微環(huán)境來加強其自身的耐鹽堿特性。
[Abstract]:In order to understand the effects of salt and alkali stress on the structure of bacteria and fungi community in the rhizosphere of cucumber grafted seedlings, In this study, two saline-alkali tolerant rootstocks, '108T1HU' and two saline-alkali-sensitive rootstocks, 'Huatarang' S1S1''Jingxin anvil No. 6 (S2) were used as test materials, and self-rooted seedlings were used as control, and the results showed that the rootstocks were sensitive to salt and alkali-sensitive rootstocks. The mixture salt (NaHCO _ 3: NaH _ 2SO _ 4: NaCl: Na _ 2CO _ 3 / T _ 4: NaCl: Na _ 2CO _ 3: 4: 2: 2: 0.15) was treated with a concentration of 100 mmol / L ~ (-1) / pH 9.0 for 20 ~ (30) d ~ ~ ~ (40) d ~ (40) d ~ (40 d) (30 d ~ (30 d) ~ 40 d ~ (50) d), and the PCR-DGGE technique was used. The effects of salt and alkali stress on microbial community structure and abundance in rhizosphere soil of cucumber seedlings grafted with different rootstocks were studied. The results show that The number of DGGE bands of saline-alkali tolerant rootstock variety T _ 1 / T _ 2 rhizosphere soil fungi was significantly higher than that of saline-alkali sensitive S _ 2 and self-rooted seedling CK, and the Shannon-Wiener index and evenness index of saline-alkali tolerant variety T _ 2 soil bacteria were significantly higher than that of saline-alkali sensitive species. S _ 1 / S _ 2 and self-rooting seedling control CK. The copy number of 16s rDNA gene of saline-alkali tolerant variety T1 was significantly higher than that of saline-alkali sensitive variety and self-rooted seedling at 50 days, and at 40 days after planting, the number of copies of 16s rDNA gene was significantly higher than that of saline-alkali sensitive variety and self-rooted seedling. The copy number of ITS gene of saline-alkali tolerant rootstock T2 fungi was significantly higher than that of saline-alkali sensitive variety and cucumber self-rooted seedling, and the ITS gene copy number of saline-alkali tolerant rootstock fungus was significantly higher than that of saline-alkali sensitive variety after 50 days of planting, but there was no significant difference with self-root seedling. The composition and abundance of microbial community in rhizosphere soil of cucumber seedlings grafted with different saline-alkali tolerant rootstocks were different. The results showed that, with the increase of saline-alkali stress time, the abundance and structure diversity of soil microbial communities of rootstocks with different saline-alkali tolerance varied greatly, which indirectly changed the soil microecological environment. The amount and richness of soil microbes also changed. Salt-tolerant rootstocks may enhance their saline-alkali tolerance by improving soil microenvironment.
【作者單位】： 東北農(nóng)業(yè)大學(xué)園藝園林學(xué)院;
【基金】：國家大宗蔬菜產(chǎn)業(yè)技術(shù)體系專項(CARS-25-08)資助~~
【分類號】：S154.3;S642.2

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本文編號：1783553