本文選題：黃瓜 + 干旱　； 參考：《應(yīng)用生態(tài)學(xué)報》2017年11期

【摘要】：以黃瓜品種‘新春4號’為材料,研究干旱脅迫下一氧化氮(NO)和鈣離子(Ca~(2+))處理下黃瓜的生根指標(biāo)、內(nèi)源Ca~(2+)熒光強(qiáng)度以及抗氧化酶(超氧化物歧化酶SOD、過氧化氫酶CAT、抗壞血酸過氧化物酶APX)活性,分析干旱條件下黃瓜不定根發(fā)生過程中NO和Ca~(2+)之間的關(guān)系.結(jié)果表明:200μmol·L~(-1)氯化鈣(CaCl_2)和0.05%聚乙二醇(PEG)共處理顯著提高了干旱條件下黃瓜不定根的根長和根數(shù);添加Ca~(2+)螯合劑(EGTA)和通道抑制劑(BAPTA/AM)處理顯著降低了干旱條件下NO誘導(dǎo)的不定根根數(shù)和根長.干旱條件下,NO和CaCl_2處理提高了黃瓜下胚軸內(nèi)源Ca~(2+)熒光強(qiáng)度;而NO清除劑(c PTIO)處理的Ca~(2+)熒光強(qiáng)度顯著低于NO處理.干旱條件下,NO和CaCl_2處理顯著提高了黃瓜下胚軸抗氧化酶活性;而Ca~(2+)抑制劑或螯合劑處理顯著降低了NO誘導(dǎo)的抗氧化酶活性.由此可見,干旱條件下Ca~(2+)參與了NO調(diào)控黃瓜抗氧化酶活性,緩解了干旱脅迫對不定根形成產(chǎn)生的傷害,進(jìn)而促進(jìn)了不定根的發(fā)生.
[Abstract]:The rooting index of cucumber (Cucumber cultivar 'Xinchun 4') was studied under drought stress (no) and Ca ~ (2 +) ~ (2 +) treatment.The fluorescence intensity of endogenous Ca~(2 and the activities of antioxidant enzymes (superoxide dismutase (SOD), catalase (cat), ascorbic acid peroxidase (Ca~(2)), and the relationship between no and Ca~(2 during adventitious root formation in cucumber under drought conditions were analyzed.The results showed that the root length and root number of adventitious roots of cucumber under drought condition were significantly increased by the co-treatment of CaCl2) and 0.05% polyethylene glycol (PEG).The addition of Ca~(2) chelating agent and channel inhibitor BAPTA / AM significantly reduced the number of adventitious roots and root length induced by no under drought conditions.The fluorescence intensity of endogenous Ca~(2 of cucumber Hypocotyl was increased by no and CaCl_2 treatments, but the fluorescence intensity of Ca~(2 treated by no scavenger was significantly lower than that of no treatment.The antioxidant enzyme activity of cucumber Hypocotyl was significantly increased by the treatment of no and CaCl_2 under drought condition, while the activity of antioxidant enzyme induced by no was significantly decreased by Ca~(2 inhibitor or chelating agent.It can be seen that Ca~(2) takes part in the regulation of antioxidant enzyme activity in cucumber under drought condition, which alleviates the injury caused by drought stress on adventitious root formation and promotes the formation of adventitious root.
【作者單位】： 甘肅農(nóng)業(yè)大學(xué)園藝學(xué)院;
【基金】：國家自然科學(xué)基金項(xiàng)目(31160398,31560563) 中國博士后科學(xué)基金項(xiàng)目(20100470887,2012T50828) 教育部科學(xué)技術(shù)研究重點(diǎn)項(xiàng)目(211182);教育部高校博士點(diǎn)新教師基金項(xiàng)目(201162020005) 甘肅省自然科學(xué)基金項(xiàng)目(1308RJZA179,1606RJZA073)資助~~
【分類號】：S423;S642.2
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本文編號：1744547