大慶市蚊發(fā)生規(guī)律及白僵菌對(duì)淡色庫(kù)蚊毒力研究
發(fā)布時(shí)間:2018-04-13 10:06
本文選題:大慶 + 蚊; 參考:《黑龍江八一農(nóng)墾大學(xué)》2015年碩士論文
【摘要】:通過(guò)研究大慶市蚊種類(lèi)、生物學(xué)特性、發(fā)生規(guī)律及球孢白僵菌對(duì)幼蟲(chóng)的防治效果,為大慶蚊子的實(shí)際防治工作提供理論依據(jù)。利用燈誘法、網(wǎng)捕法捕獲大慶市成蚊,統(tǒng)計(jì)數(shù)量,鑒定種類(lèi);室外定點(diǎn)定時(shí)調(diào)查成蚊的發(fā)生規(guī)律;實(shí)驗(yàn)室構(gòu)建淡色庫(kù)蚊種群,觀察生物學(xué)特性,研究溫度對(duì)其生長(zhǎng)發(fā)育影響,以及球孢白僵菌不同孢懸液濃度、不同孢懸液溫度對(duì)淡色庫(kù)蚊的毒力。 2014年4~10月對(duì)大慶市成蚊進(jìn)行調(diào)查,鑒定蚊種類(lèi)有4屬11種,按蚊屬(Anopheles)有中華按蚊(Anopheles sinensis)、米塞按蚊(Anopheles messeae);庫(kù)蚊屬(Culex)有淡色庫(kù)蚊(Culex pipiens pallens)、兇小庫(kù)蚊(Culex modestus)、迷走庫(kù)蚊(Culex vagans)、三帶喙庫(kù)蚊(Culex tritaeniorhynchus);伊蚊屬(Aedes)有背點(diǎn)伊蚊(Aedes dorsalis)、刺痛伊蚊(Aedes excrucians)、叮刺伊蚊(Aedes sticticus)、黑海伊蚊(Aedes cyprius);脈毛蚊屬(Culiseta)有日本脈毛蚊(Culiseta nippoaica)。大慶市廣泛分布蚊種為淡色庫(kù)蚊、兇小庫(kù)蚊、迷走庫(kù)蚊、背點(diǎn)伊蚊。優(yōu)勢(shì)蚊種淡色庫(kù)蚊占總捕獲數(shù)量的49.81%,背點(diǎn)伊蚊占總捕獲數(shù)量的18.15%。大慶市蚊始見(jiàn)期為4月20日,終見(jiàn)期為10月5日。7~8月是蚊數(shù)量發(fā)生高峰期,占總捕獲數(shù)的65.98%。4~7月優(yōu)勢(shì)蚊種為淡色庫(kù)蚊,8月優(yōu)勢(shì)蚊種為兇小庫(kù)蚊,9~10月優(yōu)勢(shì)蚊種為背點(diǎn)伊蚊。 實(shí)驗(yàn)室構(gòu)建了淡色庫(kù)蚊種群,飼養(yǎng)的關(guān)鍵技術(shù)在于嚴(yán)格控制溫度、光照時(shí)間和保持水質(zhì)清潔。明確了淡色庫(kù)蚊的雌蚊吸血習(xí)性、產(chǎn)卵習(xí)性、卵孵化習(xí)性等。淡色庫(kù)蚊的卵在15~35℃下均可正常孵化,隨著溫度提高卵孵化時(shí)間縮短。幼蟲(chóng)發(fā)育歷期隨著溫度的升高而縮短,15℃時(shí),,從1齡到羽化成蟲(chóng)需要23.62d,35℃時(shí),為7.82d,30℃時(shí),死亡率為40%,35℃時(shí),死亡率達(dá)80%。 采用不同濃度、不同溫度的球孢白僵菌孢子懸浮液處理不同蟲(chóng)齡、不同密度的淡色庫(kù)蚊幼蟲(chóng)。結(jié)果表明,球孢白僵菌孢懸液濃度與幼蟲(chóng)死亡率呈正相關(guān),相關(guān)系數(shù)為0.9947;同一孢子濃度處理下,幼蟲(chóng)死亡率隨蟲(chóng)口密度增加顯著提高;孢懸液對(duì)不同蟲(chóng)齡的致死能力差異顯著;相同孢子濃度下,4齡幼蟲(chóng)的致死率明顯低于1~3齡幼蟲(chóng);白僵菌孢懸液對(duì)幼蟲(chóng)致死率隨溫度升高顯著提高。
[Abstract]:By studying the species, biological characteristics, occurrence law and control effect of Beauveria bassiana on larvae in Daqing city, the theoretical basis for the actual control of mosquitoes in Daqing was provided.The adult mosquitoes in Daqing city were captured by lamp trap and netting method. The number of mosquitoes was counted and the species was identified. The occurrence rule of adult mosquitoes was investigated at fixed points outside. The population of Culex pipiens pallens was constructed in laboratory to observe the biological characteristics and to study the effect of temperature on its growth and development.And the virulence of Culex pipiens pallens with different suspension concentration and temperature of Beauveria bassiana.The adult mosquitoes in Daqing City were investigated from April to October of 2014, and 11 species of mosquitoes belonging to 4 genera were identified.Sticticus, Aedes priusus; Culiseta) have the genus Culiseta nippoaicia.Daqing City widely distributed species of Culex pipiens pallens, Culex pipiens, Culex vaginalis, Aedes dorsalis.The dominant mosquito species, Culex pipiens pallens, accounted for 49.81% of the total captured number, and Aedes dorsalis accounted for 18.15%.The blood-sucking habits, spawning habits and egg hatching habits of Culex pipiens pallens were clarified.The eggs of Culex pipiens pallens can hatch normally at 1535 鈩
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