大興安嶺森林火燒后不同演替階段土壤細(xì)菌多樣性動(dòng)態(tài)
發(fā)布時(shí)間:2018-04-02 13:04
本文選題:大興安嶺 切入點(diǎn):森林演替 出處:《微生物學(xué)通報(bào)》2017年08期
【摘要】:【目的】研究我國最大的林區(qū)之一——大興安嶺森林火燒后不同演替階段土壤細(xì)菌多樣性動(dòng)態(tài),為天然林保護(hù)工程對(duì)于生物多樣性的影響增添新的認(rèn)識(shí)!痉椒ā恳钥臻g替代時(shí)間的方法分析大興安嶺森林演替對(duì)于土壤細(xì)菌多樣性動(dòng)態(tài)的影響。大興安嶺森林火燒后典型的自然演替序列為火燒跡地(LG-BA)、灌叢(SHR)、白樺林(BP)、白樺落葉松混交林(BP-LG)、落葉松林(LG-OM)。在演替序列的每個(gè)典型樣地上采集0-10 cm土樣,采用Illumina Mi Seq高通量測序技術(shù)測定土壤細(xì)菌群落組成及其多樣性!窘Y(jié)果】細(xì)菌操作分類單位(OTU)數(shù)量從少到多的順序?yàn)榛馃E地落葉松白樺混交林灌叢落葉松林白樺林。隨著森林演替,多樣性指數(shù)Simpson先增高后降低;Shannon指數(shù)先增加后減少再增加;OTU的豐度變化比較平緩,表明物種變化較小。在各演替階段中,土壤細(xì)菌種類主要有變形菌門(Proteobacteria)、酸桿菌門(Acidobacteria)、放線菌門(Actinobacteria)和浮霉菌門(Planctomycetes),4個(gè)門的種類含量隨演替順序都呈現(xiàn)先增加后減少的趨勢。主成分分析表明不同演替階段土壤細(xì)菌群落存在一定的差異性。冗余分析表明有機(jī)質(zhì)(SOM)、全氮(TN)、全磷(TP)和p H對(duì)于土壤細(xì)菌群落變化有影響!窘Y(jié)論】隨著森林演替,大興安嶺地區(qū)土壤細(xì)菌種類和生物多樣性會(huì)發(fā)生變化,其變化與土壤化學(xué)成分和p H有關(guān)。
[Abstract]:[objective] to study the dynamics of soil bacterial diversity in different succession stages of Daxinganling forest, one of the largest forest regions in China. To add a new understanding of the impact of natural forest conservation engineering on biodiversity. [methods] the effects of forest succession on soil bacterial diversity dynamics in Daxinganling Mountains were analyzed by spatial substitution time. Forest Fire in Daxing'an Mountains. The typical natural succession sequence after burning was LG-BAG, SHRA, BPP, BP-LGN, LG-OM.The soil samples of 0-10 cm were collected from each typical sample of the succession sequence. Illumina Mi Seq high throughput sequencing technique was used to determine the composition and diversity of soil bacterial community. Forest. With the succession of forests, The diversity index (Simpson) increased at first, then decreased, then decreased, then increased, and then increased slowly, indicating that the variation of species was relatively small. The main species of soil bacteria were Proteobacteriae, Acidobacteriae, Actinobacteria, Actinobacteria and Planctomycetesus. The species contents of the four phylum increased first and then decreased with the succession sequence. The principal component analysis showed that the soil content increased first and then decreased in different succession stages. The results of redundancy analysis showed that organic matter (SOM), total nitrogen (TNN), total phosphorus (TP) and pH had influence on the change of soil bacterial community. [conclusion] with forest succession, soil bacterial community was changed. The species and biodiversity of soil bacteria in Daxing'anling region will change, which is related to soil chemical composition and pH.
【作者單位】: 內(nèi)蒙古農(nóng)業(yè)大學(xué)沙漠治理學(xué)院;內(nèi)蒙古大興安嶺林管局營林處;
【基金】:國家自然科學(xué)基金項(xiàng)目(No.41461106) 內(nèi)蒙古農(nóng)業(yè)大學(xué)引進(jìn)人才科研啟動(dòng)項(xiàng)目(No.YJ2014-1)~~
【分類號(hào)】:S714.3
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