本文選題：膠孢炭疽菌　切入點(diǎn)：放線菌　出處：《農(nóng)藥學(xué)學(xué)報(bào)》2017年06期

【摘要】：從山東省臨沂地區(qū)土壤中分離到一株放線菌gz-8,發(fā)現(xiàn)其對膠孢炭疽菌Colletotrichum gloeosporioides具有較強(qiáng)的抑制作用,皿內(nèi)抑菌活性達(dá)72.5%。通過形態(tài)特征和生理生化測定,結(jié)合16S r DNA序列分析,鑒定該菌株為栗褐鏈霉菌Streptomyces badius�？咕V測定結(jié)果表明,菌株gz-8對6種植物病原真菌均具有較強(qiáng)的抑菌活性,抗菌譜較廣。進(jìn)一步對菌株gz-8的粗提物進(jìn)行了生物活性評價,發(fā)現(xiàn)其發(fā)酵液粗提物可以明顯抑制膠孢炭疽菌的菌絲生長(EC50=3.18μg/m L)和分生孢子萌發(fā)(IC50=1.92μg/m L),且活性明顯高于菌體粗提物;同時發(fā)酵液粗提物具有良好的熱穩(wěn)定性及p H穩(wěn)定性;1 000 mg/L的發(fā)酵液粗提物對感染膠孢炭疽病芒果果實(shí)的防效達(dá)到35.94%,與同濃度下百菌清的防效(34.11%)無顯著差異。
[Abstract]:A strain of actinomycetes gz-8 was isolated from the soil of Linyi area, Shandong Province. It was found that it had a strong inhibitory effect on Colletotrichum gloeosporioides, and its antibacterial activity reached 72.5% in dish. By means of morphological characteristics and physiological and biochemical analysis, combined with 16s r DNA sequence analysis, The strain was identified as Streptomyces chrysostreptomyces Streptomyces badius. The results of antimicrobial spectrum analysis showed that the strain gz-8 had strong antibacterial activity against 6 plant pathogenic fungi, and the antibacterial spectrum was wide. Further, the bioactivity of crude extract of gz-8 was evaluated. It was found that the crude extract could inhibit the mycelium growth of Bacillus anthracis (EC5018 渭 g / mL) and the conidia germination of IC500.92 渭 g / mL, and its activity was significantly higher than that of the crude bacterial extract. At the same time, the crude extract of fermentation broth had good thermal stability and pH stability of 1 000 mg/L. The control effect of crude extract on mango fruit infected with gum anthracnose reached 35.94. There was no significant difference between the crude extract of fermentation broth and chlorothalonil at the same concentration.
【作者單位】： 海南大學(xué)熱帶農(nóng)林學(xué)院;
【基金】：海南省重點(diǎn)研發(fā)計(jì)劃(ZDYF2016155) 國家自然科學(xué)基金(31560045)
【分類號】：S476.1

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