本文選題：養(yǎng)殖魚塘　切入點(diǎn)：微生物　出處：《環(huán)境科學(xué)學(xué)報(bào)》2017年10期

【摘要】：針對(duì)水產(chǎn)養(yǎng)殖中抗生素濫用引起微生物耐藥基因污染的現(xiàn)象,本實(shí)驗(yàn)以杭州市某水產(chǎn)養(yǎng)殖區(qū)底泥樣品為研究對(duì)象,測(cè)定了底泥樣品中抗生素含量,并從其中分離出74株可培養(yǎng)細(xì)菌.采用PCR方法對(duì)分離菌株中磺胺類、四環(huán)素類、喹諾酮類、氯霉素類抗性基因(antibiotic resistance genes,ARGs)和整合子基因進(jìn)行了檢測(cè).結(jié)果表明,除tet A(四環(huán)素類)耐藥基因未被檢出外,其余3種耐藥基因及整合子基因均被檢出,其中qnr S(喹諾酮類)耐藥基因的檢出率最高,為50.00%.另外,利用16S r DNA序列分析技術(shù)將分離菌株鑒定為12個(gè)屬、19個(gè)種,包括環(huán)境中多種常見土著細(xì)菌及部分致病菌,其中氣單胞菌(Aeromonas spp.)數(shù)量最多(29株),占分離菌株的39.19%.掌握水產(chǎn)養(yǎng)殖區(qū)中ARGs的污染現(xiàn)狀,對(duì)控制其傳播、保障食品安全和保護(hù)微生態(tài)環(huán)境具有重要指導(dǎo)意義.
[Abstract]:In view of the phenomenon of microbial drug resistance gene contamination caused by antibiotic abuse in aquaculture, the content of antibiotics in sediment samples in a aquaculture area of Hangzhou was determined. 74 strains of culturable bacteria were isolated from them. Sulfonamides, tetracyclines, quinolones, chloramphenicol resistant genes and integron genes were detected by PCR. Except tet A (tetracycline) resistance gene was not detected, the other three drug resistance genes and integron genes were detected. The detection rate of qnr S (quinolones) resistance gene was the highest (50.00g). The isolated strains were identified into 12 genera and 19 species by 16s r DNA sequence analysis, including a variety of common indigenous bacteria and some pathogenic bacteria in the environment. Among them, Aeromonas spp.has the largest number of 29 strains, accounting for 39.19% of the isolated strains. It is of great significance to grasp the status of ARGs pollution in aquaculture area, to control its spread, to ensure food safety and to protect the microecological environment.
【作者單位】： 中國計(jì)量大學(xué)生命科學(xué)學(xué)院;浙江省海洋食品品質(zhì)及危害物質(zhì)控制技術(shù)重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金(No.31601464) 浙江省自然科學(xué)基金項(xiàng)目(No.LQ15C200001)~~
【分類號(hào)】：X172;X714
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本文編號(hào)：1673979