常溫降解玉米秸稈復(fù)合菌系及其微生物多樣性研究
發(fā)布時間:2018-03-26 19:16
本文選題:常溫 切入點:復(fù)合菌系 出處:《可再生能源》2017年08期
【摘要】:為了能夠在常溫條件下高效降解玉米秸稈,從常年種植玉米的農(nóng)田土壤中選取土樣,通過連續(xù)的限制性培養(yǎng),以纖維素降解率為篩選主要指標(biāo),得到了一組高效纖維素分解復(fù)合菌系。復(fù)合菌系木質(zhì)纖維素最適的分解溫度為30℃,p H值為8,此時濾紙和玉米秸稈的降解率分別為66.5%和66.1%,玉米秸稈中纖維素、半纖維素和木質(zhì)素的降解率分別為17.0%,85.1%和34.4%。通過高通量測序方法分析了復(fù)合菌系的微生物多樣性,結(jié)果表明:在屬的水平上,復(fù)合菌系主要由33.9%假單胞菌屬(Pseudomonas),11.8%擬桿菌屬(Bacteroides),6.9%食酸菌屬(Acidovorax),3.8%產(chǎn)堿桿菌屬(Alcaligenes),1%丁酸弧菌屬(Butyrivibrio)等組成;在種的水平上,菌系主要由2%糞產(chǎn)堿桿菌(Alcallgenes-faecalis),1.5%空氣除氮微枝桿菌(Microvirgula-aerodenitrificans)等組成。常溫復(fù)合菌系能高效分解纖維素、半纖維素等成分,在寒區(qū)農(nóng)業(yè)廢棄物資源化利用方面有著一定的應(yīng)用潛力。
[Abstract]:In order to degrade corn straw efficiently under normal temperature condition, soil samples were selected from the farmland soil where maize was planted all the year round. The degradation rate of cellulose was taken as the main index through continuous restricted culture. The optimum decomposition temperature of lignocellulose was 30 鈩,
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