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堆肥過程中纖維素酶活與纖維素降解相關(guān)研究

發(fā)布時間:2018-03-13 16:18

  本文選題:堆肥 切入點(diǎn):CMC酶 出處:《東北農(nóng)業(yè)大學(xué)學(xué)報(bào)》2016年06期  論文類型:期刊論文


【摘要】:采用室內(nèi)小型堆肥反應(yīng)器堆肥,測定CMC酶活性和β-葡萄糖苷水解酶活性,測定堆肥物料中纖維素、半纖維素和木質(zhì)素相對含量,利用高效液相法測定葡萄糖和纖維二糖。結(jié)果表明,堆肥過程中,堆體溫度前3 d迅速上升至45℃,進(jìn)入高溫期,維持8 d后進(jìn)入降溫期,最后與環(huán)境溫度變化一致;β-葡萄糖苷水解酶活性第4天達(dá)最大值(1.482μmol p-Nitr·g-1DW·min-1),并在降溫腐熟期維持在0.429~0.533μmol p-Nitr·g-1DW·min-1范圍內(nèi);CMC酶活性變化趨勢與β-葡萄糖苷水解酶活性相似,并在第7天達(dá)最大值(47.67μg glucose·g-1DW·min-1);纖維素和半纖維素降解趨勢相似,均在高溫期降解明顯;木質(zhì)素相對含量總體變化較小,且在降溫腐熟期小幅增加。結(jié)果顯示,CMC酶活性與纖維素和半纖維素降解呈正相關(guān)。
[Abstract]:The enzyme activities of CMC and 尾 -glucoside hydrolase were determined by composting in a small composting reactor, and the relative contents of cellulose, hemicellulose and lignin in compost materials were determined. Glucose and cellobiose were determined by high performance liquid phase method. The results showed that during composting, the first 3 days of composting temperature increased rapidly to 45 鈩,

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