本文選題：皂角苷　切入點(diǎn)：林丹　出處：《西南農(nóng)業(yè)學(xué)報(bào)》2017年05期 　論文類型：期刊論文

【摘要】：【目的】為了提高環(huán)境中持久性有機(jī)氯農(nóng)藥的生物降解效果�！痉椒ā坷闷胶庹袷幏ㄑ芯苛松锉砻婊钚詣┰斫擒諏�(duì)有機(jī)氯農(nóng)藥林丹的增溶作用,并通過液相培養(yǎng)法研究了皂角苷對(duì)白腐真菌Phlebia brevispora生長以及降解林丹的強(qiáng)化效果�！窘Y(jié)果】皂角苷能大幅度增加林丹的溶解度,實(shí)驗(yàn)濃度范圍內(nèi)增溶倍數(shù)最高可達(dá)到7.4。皂角苷作為生長基質(zhì)可促進(jìn)P.brevispora的生長,提高其生物量,但濃度在達(dá)到1.0 g/L時(shí),菌株生物量幾乎不再繼續(xù)增加。在0.05~1.0 g/L濃度范圍內(nèi),皂角苷能顯著提高菌株對(duì)溶液中林丹的降解效果(P0.05),且降解率隨著皂角苷濃度的增加而增加,降解率最大可提高14.1%。添加0.5和1.0 g/L的皂角苷處理時(shí),菌株胞內(nèi)粗酶液對(duì)林丹的降解率顯著高于不添加皂角苷的處理(P0.05)。皂角苷對(duì)林丹的增溶效果隨著無機(jī)鹽的加入而提高,20 mmol/L的Na Cl和Na_2SO_4使林丹的溶解度提高了1.17和1.26倍;而在含有皂角苷的降解體系中,Na Cl的添加進(jìn)一步促進(jìn)了林丹的生物降解效果,降解率提高了10%以上�！窘Y(jié)論】本研究結(jié)果表明,利用皂角苷強(qiáng)化微生物降解去除環(huán)境中的有機(jī)污染物是可行的,具有一定的實(shí)際應(yīng)用價(jià)值。
[Abstract]:[objective] to improve the biodegradation of persistent organochlorine pesticides in the environment. [methods] the solubilization of biosaponin on Lin Dan was studied by equilibrium oscillation method. The effects of saponins on the growth of white rot fungus Phlebia brevispora and the degradation of Lin Dan were studied by liquid phase culture. [results] saponin could significantly increase the solubility of Lin Dan. The solubilization ratio of P. brevispora was up to 7.4 in the range of experimental concentration. Saponin could promote the growth and increase the biomass of P.brevispora, but when the concentration reached 1.0 g / L, the biomass of P. brevispora was almost no longer increased, and in the range of 0.05g / L, the biomass of P. brevispora was almost no longer increased. Saponins could significantly improve the degradation of Lin Dan in the solution, and the degradation rate increased with the increase of saponin concentration, and the degradation rate was increased by 14.1g / L. when 0.5 and 1.0 g / L saponins were added, the degradation rate increased with the increase of the concentration of saponin. The degradation rate of Lin Dan by crude enzyme solution was significantly higher than that without saponin. The solubilization effect of saponins on Lin Dan increased by 1.17 and 1.26 times with the addition of inorganic salt, NaCl and Na_2SO_4 for 20 mmol/L. In the degradation system containing saponins, the biodegradation effect of Lin Dan was further promoted by the addition of sodium chloride, and the degradation rate was increased by more than 10%. [conclusion] the results of this study show that the biodegradation rate of Lin Dan is more than 10%. It is feasible to use saponin to enhance microbial degradation and removal of organic pollutants in the environment, which has certain practical application value.
【作者單位】： 東北林業(yè)大學(xué)林學(xué)院;九州大學(xué)農(nóng)學(xué)院;
【基金】：國家自然科學(xué)基金項(xiàng)目(41201307) 教育部留學(xué)回國人員科研啟動(dòng)基金([2013]693)
【分類號(hào)】：X172;X592

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本文編號(hào)：1599360