雞糞除臭菌的篩
發(fā)布時(shí)間:2018-03-02 04:19
本文關(guān)鍵詞: 環(huán)境工程學(xué) 除臭 紅平紅球菌 培養(yǎng)條件 出處:《安全與環(huán)境學(xué)報(bào)》2017年01期 論文類型:期刊論文
【摘要】:為了篩選出具有高效除臭能力的菌株,采用馴化富集、平板劃線的方法從雞糞中篩選除臭微生物,采用初篩、復(fù)篩相結(jié)合的方法檢測所篩選微生物的除臭效果。初篩采用嗅閾值法,復(fù)篩以氨氣和硫化氫的去除率為檢測指標(biāo),最終獲得除臭效果最好的菌株CCJZ022。經(jīng)過形態(tài)學(xué)觀察、生理生化鑒定和16S rRNA基因序列分析,確定菌株CCJZ022為紅平紅球菌(Rhodococcus erythropolis)。對其初始pH值、培養(yǎng)溫度及接種量進(jìn)行優(yōu)化,發(fā)現(xiàn)該菌株在初始pH值為7.0、培養(yǎng)溫度為30℃、接種量為12%時(shí)除臭效果最好,對氨氣和硫化氫的去除率分別可達(dá)66.73%和54.51%。利用該菌株進(jìn)行雞糞除臭的小試也取得了很好的效果,表明該菌株可以應(yīng)用于雞糞除臭。
[Abstract]:In order to screen the strains with high deodorization ability, the bacteria were screened from chicken dung by domestication and enrichment. The combined screening method was used to detect the deodorization effect of the selected microorganism. The primary screening method was based on the olfactory threshold method, and the removal rate of ammonia and hydrogen sulfide was taken as the detection index. Finally, the strain CCJZ022, which had the best deodorization effect, was obtained. Physiological and biochemical identification and 16s rRNA gene sequence analysis showed that the strain CCJZ022 was Rhodococcus erythropolisus. The initial pH value, culture temperature and inoculation amount of the strain were optimized. It was found that the initial pH value was 7.0 and the culture temperature was 30 鈩,
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