本文關(guān)鍵詞： Bt棉 澇漬 殺蟲蛋白 氮代謝　出處：《作物學(xué)報(bào)》2017年11期 　論文類型：期刊論文

【摘要】：以Bt棉常規(guī)種泗抗1號和雜交種泗抗3號為材料,于盛蕾期設(shè)計(jì)不同程度澇漬(土壤持水量為最大持水量的90%、100%,漬水2 cm)脅迫4 d以及土壤持水量為最大持水量90%逆境下持續(xù)脅迫24~120 h的試驗(yàn),探討棉蕾中Bt殺蟲蛋白表達(dá)量變化及相關(guān)氮代謝生理機(jī)制。結(jié)果表明,土壤不同澇漬程度影響棉蕾Bt蛋白含量。土壤持水量為最大持水量的90%、100%和漬水2 cm處理脅迫后,常規(guī)種泗抗1號棉蕾中Bt蛋白含量分別下降36.5%、42.1%和51.4%,雜交種泗抗3號分別下降19.2%、57.2%和64.5%。90%的土壤持水量持續(xù)脅迫96 h使棉蕾Bt蛋白含量開始顯著下降,泗抗1號和泗抗3號分別下降20.8%和17.6%。隨著脅迫時(shí)間延長下降幅度增大。不同澇漬程度或水漬持續(xù)脅迫條件下,棉蕾中可溶性蛋白含量,硝酸還原酶(NR)、谷氨酸草酰乙酸轉(zhuǎn)氨酶(GOT)、谷氨酸丙酮酸轉(zhuǎn)氨酶(GPT)、谷氨酰胺合成酶(GS)和谷氨酸合成酶(GOGAT)活性下降,游離性氨基酸含量,肽酶、蛋白酶活性增加。說明澇漬逆境下,棉蕾中蛋白質(zhì)合成能力下降、分解能力增強(qiáng),引起可溶性蛋白包括Bt殺蟲蛋白含量下降,導(dǎo)致棉蕾抗蟲性下降。
[Abstract]:The conventional BT cotton strain Sikang1 and the hybrid Sikang3 were used as materials. The experiment of waterlogging (90 ~ 100 cm of maximum soil water holding capacity, 2 cm of soil waterlogging) was designed at the stage of Sheng Bud. The experiment was carried out for 4 days under the stress of soil water holding capacity being the maximum water holding capacity of 90%, and the continuous stress was 24 ~ 120h under the stress of 90%. The changes of BT insecticidal protein expression in cotton bud and the related physiological mechanism of nitrogen metabolism were studied. The results showed that different soil waterlogging degree affected the BT protein content of cotton bud, and the soil water holding capacity was 90% of the maximum water holding capacity and 2 cm of waterlogging. The content of BT protein in cotton bud of Sikang No. 1 decreased by 36.52.1% and 51.4%, respectively, and that of hybrid sikang 3 decreased by 19.2% and 64.57.2% and 64.50.90%, respectively, and the content of BT protein in cotton bud began to decrease significantly after 96 h of continuous stress. Sikang 1 and Sikang 3 decreased by 20.8% and 17.6 respectively. The content of soluble protein in cotton bud increased with the prolongation of stress time. The activities of nitrate reductase (NRN), glutamic acid oxaloacetic acid transaminase (glutamic acid oxaloacetic acid transaminase), glutamic acid pyruvate transaminase (GPTN), glutamine synthase (GSG) and glutamate synthase (GOGATT) decreased, and the contents of free amino acids, peptidase and protease activities increased. The protein synthesis ability and decomposition ability of cotton bud decreased, which resulted in the decrease of soluble protein, including BT insecticidal protein content, which resulted in the decrease of cotton bud insecticidal resistance.
【作者單位】： 揚(yáng)州大學(xué)江蘇省作物遺傳生理重點(diǎn)實(shí)驗(yàn)室;中國農(nóng)業(yè)科學(xué)院棉花研究所棉花生物學(xué)國家重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金項(xiàng)目(31471435,31301263,31671613) 江蘇省三新工程項(xiàng)目[SXGC(2014)317,(2016)320] 江蘇省高等教育優(yōu)勢學(xué)科發(fā)展計(jì)劃(PAPD) 中國農(nóng)業(yè)科學(xué)院棉花研究所植棉技術(shù)標(biāo)準(zhǔn)化研究與示范團(tuán)隊(duì)項(xiàng)目(2016PCTS-1)資助~~
【分類號】：S422;S562

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