本文關(guān)鍵詞： Illumina Mi Seq高通量測(cè)序 鹽堿土 河西走廊　出處：《微生物學(xué)通報(bào)》2017年09期 　論文類型：期刊論文

【摘要】：【目的】以甘肅省河西走廊地區(qū)的9個(gè)鹽堿土壤樣品(原生鹽堿土、次生鹽堿土、農(nóng)田土)為材料,研究該地區(qū)鹽堿土壤中微生物群落的多樣性�！痉椒ā刻崛⊥寥牢⑸锟侱NA,應(yīng)用Illumina Mi Seq高通量測(cè)序技術(shù)進(jìn)行分析。【結(jié)果】從分布在河西走廊3個(gè)流域的9個(gè)鹽堿土樣品中共獲得325 089條微生物的16S r RNA基因序列。冗余分析和熱圖分析表明,原生鹽堿土與次生鹽堿土、原生鹽堿土與農(nóng)田土微生物群落構(gòu)成差異較大,次生鹽堿土與農(nóng)田土微生物群落差異較小。土壤p H對(duì)微生物群落組成的影響最顯著。多樣性指數(shù)和稀釋性曲線分析得出,在9個(gè)土壤樣品中,S6號(hào)Shannon指數(shù)最大,S1號(hào)Shannon指數(shù)最小,S1號(hào)Simpson指數(shù)最大,S6號(hào)Simpson指數(shù)最小,說(shuō)明原生鹽堿土的微生物群落多樣性最低,次生鹽堿土的微生物群落多樣性最高。鹽堿土壤中主要的微生物群落包括9個(gè)門,其中變形菌門占主導(dǎo)地位,其余依次是放線菌門、擬桿菌門、酸桿菌門、浮霉菌門、綠彎菌門、芽單胞菌門、厚壁菌門和疣微菌門。原生鹽堿土和農(nóng)田土中占優(yōu)勢(shì)的微生物群落是變形菌門,次生鹽堿土中占優(yōu)勢(shì)的微生物群落是放線菌門�！窘Y(jié)論】河西走廊地區(qū)鹽堿土壤中微生物多樣性非常豐富,存在大量的微生物類群,尤其是在次生鹽堿土壤中。
[Abstract]:[objective] to study 9 saline-alkali soil samples (primary saline-alkali soil, secondary saline-alkali soil, farmland soil) from Hexi Corridor, Gansu Province, China. The diversity of microbial communities in saline-alkali soils in this area was studied. [methods] Total microbial DNA was extracted and analyzed by Illumina Mi Seq high-throughput sequencing technique. [results] Nine saline-alkali soils distributed in three basins of Hexi Corridor were analyzed. The 16s r RNA gene sequence of 325,089 microbes was obtained. The composition of microbial communities in primary saline soil and secondary saline alkali soil is different from that in farmland soil. The difference of microbial community between secondary saline-alkali soil and farmland soil was small, the effect of soil pH on the composition of microbial community was most significant. The diversity index and dilution curve analysis showed that, In 9 soil samples, the maximum Shannon index of S6 and the minimum Shannon index of S1 the largest Simpson index of S1 and the lowest Simpson index of S6 indicated that the diversity of microbial communities in the native saline and alkali soils was the lowest. The diversity of microbial community in secondary saline-alkali soil is the highest. The main microbial communities in saline-alkali soil include 9 phylum, among which Proteus is dominant, and the others are actinomycetes, Bacteroides, acidobacterium, floatula phylum, etc. Phylum, phylum, phylum, Microphylum, phylum verruca. Actinomycetes is the dominant microbial community in the secondary saline-alkali soil. [conclusion] the microbial diversity in the saline-alkali soil in Hexi Corridor is very rich, and there are a large number of microbial groups, especially in the secondary saline-alkali soil.
【作者單位】： 西北師范大學(xué)生命科學(xué)學(xué)院;甘肅農(nóng)業(yè)大學(xué)草業(yè)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(No.31260134)~~
【分類號(hào)】：S154.3

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