本文關(guān)鍵詞： 外生菌根菌 樟子松 生理指標(biāo) 土壤酶　出處：《生態(tài)學(xué)雜志》2017年11期 　論文類型：期刊論文

【摘要】：本研究采用盆栽試驗(yàn)法,研究了不同濃度Cd脅迫下,接種外生菌根菌——褐環(huán)乳牛肝菌(Suillus luteus)對(duì)樟子松(Pinus sylvestris var.mongolica)苗期植株的生長(zhǎng)及葉綠素含量、抗氧化酶(CAT、POD和SOD)活性、細(xì)胞滲透調(diào)節(jié)物質(zhì)(可溶性糖、MDA和游離脯氨酸)含量和根際土壤酶(過(guò)氧化氫酶、脲酶和堿性磷酸酶)活性的變化。結(jié)果表明:1年生樟子松苗木在Cd脅迫1個(gè)月后,接菌處理組無(wú)論在苗木生長(zhǎng)、抗氧化酶活性方面均高于對(duì)照組,且大部分指標(biāo)達(dá)到顯著差異水平(P0.05)。各接菌處理組菌根侵染率達(dá)48.0%以上�？寡趸富钚苑矫�,CAT和POD活性呈現(xiàn)出隨著脅迫程度的加深,先下降后上升的變化趨勢(shì),SOD活性則呈現(xiàn)出地上與地下部分"上下對(duì)稱"的變化趨勢(shì)。同時(shí),通過(guò)對(duì)各處理組苗木細(xì)胞滲透調(diào)節(jié)物質(zhì)的定量測(cè)定,接菌處理組的細(xì)胞受破壞程度顯著低于不接菌處理組。對(duì)苗木根際土壤酶分析結(jié)果表明,Cd脅迫下,施加外生菌根菌可以有效提高樟子松根際土壤酶的活性(尤其是脲酶),進(jìn)而起到增加樟子松根際土壤生物活性作用。綜上,接種外生菌根真菌對(duì)樟子松緩解重金屬Cd脅迫具有明顯的促進(jìn)作用。Cd脅迫下,外生菌根可以有效正向調(diào)節(jié)樟子松的生長(zhǎng)、抗氧化脅迫能力并提高苗木細(xì)胞滲透調(diào)節(jié)作用,進(jìn)而提高苗木對(duì)重金屬的耐受性和提高苗木根際土壤生物活性。本研究可以為重金屬污染土地的有效治理和利用木本植物修復(fù)改良重金屬污染土壤提供參考。
[Abstract]:In this study, pot experiment was used to study the effects of CD stress on different concentrations of CD. Inoculation of ectomycorrhizal fungi, Suillus luteus, against Pinus sylvestris var. mongolica. Seedling growth and chlorophyll content. The activities of antioxidant enzymes such as POD and SOD, the content of cellular osmotic regulators (soluble sugar malondialdehyde (MDA) and free proline) and the rhizosphere soil enzymes (catalase) were observed. The changes of urease and alkaline phosphatase activities. The results showed that the activities of antioxidant enzymes in seedlings of 1-year-old Pinus sylvestris var. mongolica seedlings were higher than those of control group after 1 month of CD stress. And most of the indexes reached significant difference level (P0.05). The mycorrhizal infection rate of each inoculation group was more than 48.0%, and the antioxidant enzyme activity was more than 48.0%. The activity of CAT and POD showed a tendency of decreasing first and then rising with the deepening of stress, while the activity of CAT showed a tendency of "up and down symmetry" between above and below. Through the quantitative determination of osmotic regulatory substances in seedling cells of each treatment group, the degree of cell damage in inoculated group was significantly lower than that in non-inoculated group. The results of enzyme analysis in rhizosphere soil of seedlings showed that under CD stress. The application of ectomycorrhizal fungi can effectively increase the soil enzyme activity (especially urease) of Pinus sylvestris var. mongolica rhizosphere, and then increase the biological activity of rhizosphere soil of Pinus sylvestris var. mongolica. Inoculation of ectomycorrhizal fungi could significantly promote the alleviation of CD stress of Pinus sylvestris var. mongolica. Under CD stress, ectomycorrhizal fungi could positively regulate the growth of Pinus sylvestris var. mongolica. The ability of antioxidant stress and the osmotic regulation of seedling cells were improved. This study can provide a reference for the effective treatment of heavy metal contaminated land and the remediation and improvement of heavy metal contaminated soil by woody plants.
【作者單位】： 沈陽(yáng)農(nóng)業(yè)大學(xué)林學(xué)院;黑龍江省林科院森林保護(hù)研究所;
【基金】：沈陽(yáng)農(nóng)業(yè)大學(xué)引進(jìn)人才啟動(dòng)項(xiàng)目(2015年) 黑龍江林科院人才培養(yǎng)基金項(xiàng)目(2015R01) 國(guó)家自然科學(xué)基金項(xiàng)目(31670649,31200484,31700564) 黑龍江省級(jí)基本科研業(yè)務(wù)費(fèi)項(xiàng)目(黑龍江省財(cái)政廳項(xiàng)目)資助
【分類號(hào)】：S714.3;S791.253
【正文快照】： 鎘(Cd)污染是眾多的環(huán)境問(wèn)題當(dāng)中尤為重要的一類(劉廣深等,2004;徐明崗等,2008)。土壤中的Cd主要來(lái)自化學(xué)工業(yè)排放的廢水,城市垃圾的燃燒(He et al.,2013),含重金屬農(nóng)藥的使用等(崔玉靜等,2003;李靜等,2008)。Cd對(duì)植物具有毒性,影響植物的生理生化過(guò)程(Sobkowiak et al.,2004;

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