本文選題：氧化劑 + 氧化亞鐵硫桿菌��； 參考：《南華大學(xué)》2015年碩士論文

【摘要】：隨著工業(yè)生產(chǎn)的發(fā)展,礦產(chǎn)資源的需求量不斷增加,其開采規(guī)模越來(lái)越大。深部礦體、低品位礦體、復(fù)雜條件礦體已逐漸成為主要的開采加工對(duì)象。針對(duì)這些開采對(duì)象,傳統(tǒng)的采選方法難以實(shí)現(xiàn)經(jīng)濟(jì)高效、安全、環(huán)保的生產(chǎn)要求。微生物浸出技術(shù)具有彌補(bǔ)傳統(tǒng)采選方法的這一缺點(diǎn),生物氧化劑的制備是微生物浸出技術(shù)的關(guān)鍵。本實(shí)驗(yàn)以海藻酸鈉-聚丙烯酰胺兩種混合材料為載體,并加入極易溶于水的聚乙二醇400(PEG)為致孔劑,以Ca Cl2、檸檬酸鋁為交聯(lián)劑,用包埋法固定A.f菌并構(gòu)建固定床生物反應(yīng)器以生產(chǎn)Fe3+制備微生物氧化劑。為了制備具有高氧化能力的微生物氧化劑,本實(shí)驗(yàn)的主要研究?jī)?nèi)容如下:(1)通過條件實(shí)驗(yàn),選擇出了A.f菌的最佳包埋條件為:海藻酸鈉濃度為2.5%,聚丙烯酰胺濃度為0.4%,聚乙二醇400濃度為2%。在此條件下所制得的固定化A.f菌Fe2+的最大氧化速率為2.863g·(L·h)-1。凝膠小球內(nèi)A.f菌增值比例可達(dá)到1.51倍。固定化A.f菌重復(fù)使用15d后,依然保持較好的氧化能力。(2)以選擇的最佳固定化條件包埋A·f菌制備大量凝膠微球,裝入自制的固定床生物反應(yīng)器內(nèi),通過條件實(shí)驗(yàn)獲得了固定床生物反應(yīng)器的最佳運(yùn)行條件為:空氣流量為1.4 L·min-1、初始p H值為2.0、反應(yīng)器外層恒溫夾套水溫為32℃、初始Fe2+濃度為8.5 g·L-1、稀釋率為0.6 h-1。反應(yīng)器在該條件下連續(xù)運(yùn)行,得到Fe2+的最大氧化速率為2.97 g·L-1·h-1,是游離態(tài)A·f菌的3.38倍。反應(yīng)器開始連續(xù)運(yùn)行15d后,對(duì)Fe2+的氧化能力略有降低,通過清洗反應(yīng)器內(nèi)沉淀物質(zhì)后,固定化A·f菌的氧化能力恢復(fù)84%,實(shí)驗(yàn)表明固定床生物反應(yīng)器穩(wěn)定性較好。最終實(shí)驗(yàn)通過分析獲得固定化A·f菌對(duì)Fe2+的一級(jí)反應(yīng)速率常數(shù)為k=0.39 h-1。
[Abstract]:With the development of industrial production, the demand for mineral resources is increasing. Deep ore body, low grade ore body and complex ore body have gradually become the main objects of mining and processing. For these mining objects, the traditional mining methods are difficult to meet the requirements of economic, efficient, safe and environmental protection. Microbial leaching technology has the disadvantage of traditional extraction and separation, and the preparation of biological oxidant is the key of microbial leaching technology. In this experiment, sodium alginate and polyacrylamide were used as the carrier, polyethyleneglycol 400 (PEG), which was easily soluble in water, was added as porous agent, and calcium chloride _ 2 and aluminum citrate were used as crosslinking agents. A fixed bed bioreactor was constructed to produce Fe3 to produce microbial oxidant. In order to prepare microbial oxidant with high oxidation ability, the main contents of this experiment are as follows: (1) through conditional experiment, The optimum embedding conditions of A. F bacteria were as follows: sodium alginate concentration was 2.5, polyacrylamide concentration was 0.4, polyethylene glycol 400 concentration was 2. 5%. Under these conditions, the maximum oxidation rate of the immobilized A. f strain Fe2 was 2.863 g (L h) -1. The increment ratio of A. f bacteria in gel pellets can reach 1.51 times. After repeated use for 15 days, the immobilized A. F bacteria still maintained good oxidation ability. (2) A large number of gel microspheres were prepared by embedding A f bacteria under the optimum immobilization conditions, and were placed in a self-made fixed-bed bioreactor. The optimal operating conditions of the fixed bed bioreactor were obtained by the condition experiment: the air flow rate was 1.4 L min -1, the initial pH value was 2.0, the water temperature of the jacket was 32 鈩，

本文編號(hào)：2073012