本文選題：煤炭 + 二苯并噻吩��； 參考：《昆明理工大學(xué)》2015年碩士論文

【摘要】：高硫煤炭在燃燒過程中產(chǎn)生大量的SO2氣體,嚴(yán)重污染環(huán)境,雖然用物理和化學(xué)法能將高硫煤進行脫硫處理,但從環(huán)境友好和經(jīng)濟效益來看,它們并不是最好的選擇。利用微生物技術(shù)降低煤炭中有機硫含量,反應(yīng)條件溫和、對環(huán)境友好,因此開展微生物煤炭脫硫技術(shù)的研究與開發(fā)對煤炭產(chǎn)業(yè)發(fā)展意義重大。本論文從云南省的圭山、小龍?zhí)逗鸵肆即髽驕厦旱V礦區(qū)采集了不同的煤炭樣品,同時以污水處理廠活性污泥為起始菌的來源。首先利用活性污泥中的混合菌處理煤炭樣品,在處理過程中對培養(yǎng)基、培養(yǎng)溫度、pH等都進行了優(yōu)化,但始終沒有看到明顯的有機硫降解效果。隨后利用DBT為底物,利用無限稀釋法和選擇性培養(yǎng)基分離得到了8株細(xì)菌,對8株菌進行了形態(tài)學(xué)觀察及16S rRNA基因測序,根據(jù)16S rRNA基因序列比對結(jié)果將其初步鑒定為：根瘤菌屬(Rhizobiumsp.)、黃色桿菌屬(Xanthobacter sp.)、紅串紅球菌(Rhodococcus erythropolis)、紅球菌屬(Rhodococcus sp.)、假單胞菌屬(Pseudomonas sp.)、節(jié)桿菌屬(Arthrobacter sp.)和莫拉克斯氏菌屬(Moraxella sp.)的菌株。以DBT為底物,利用高效液相色譜法對這8株菌的降解能力進行了評估,發(fā)現(xiàn)6株能夠降解DBT(Dibenzothiophene),其中HWBHT2菌培養(yǎng)3d后的DBT降解率為45.8%；XFJ2菌培養(yǎng)7d后DBT的降解率為76.2%。隨后利用菌株XFJ2和HWBHT2對云南煙煤進行了脫有機硫的處理,處理結(jié)果委托云南省煤炭產(chǎn)品質(zhì)量監(jiān)督檢驗站進行了檢測,但發(fā)現(xiàn)這兩株菌對云南煙煤中的有機硫不能降解,盡管對培養(yǎng)基和生理生化條件的進行了改進和優(yōu)化,依然未能降低煤中的有機硫含量。究其原因：(1)云南煙煤中的有機硫可能并非DBT類物質(zhì)；(2)云南煙煤中的其它成分對細(xì)菌的生長和代謝具有抑制作用。本研究共分離得到6株能夠降解DBT的細(xì)菌,并研究了其降解DBT的能力,為進一步開展煤炭脫除有機硫的實際應(yīng)用提供了一定的理論和實踐依據(jù),但利用微生物脫除煤炭中的有機硫還有待進一步研究。
[Abstract]:High sulfur coal produces a large amount of SO2 gas in the combustion process, which pollutes the environment seriously. Although it can be desulphurized by physical and chemical methods, it is not the best choice from the point of view of environmental friendliness and economic benefit. Using microbial technology to reduce the content of organic sulfur in coal, the reaction conditions are mild and friendly to the environment. Therefore, it is of great significance to develop microbial coal desulphurization technology for the development of coal industry. In this paper, different coal samples were collected from Guishan, Xiaolongtan and Yiliangdaogou coal mines in Yunnan Province. At first, the mixed bacteria in activated sludge were used to treat coal samples. The medium, culture temperature and pH were optimized in the process of treatment, but no obvious degradation effect of organic sulfur was observed. Eight strains of bacteria were isolated by infinite dilution method and selective medium using DBT as substrate. Morphological observation and 16s rRNA gene sequencing of 8 strains were carried out. According to the results of 16s rRNA gene sequence alignment, it was identified as Rhizobium sp., Xanthobacter sp., Rhodococcus erythropolisus, Rhodococcus sp., Pseudomonas sp. and Arthrobacter sp.). Moraxella sp. and Moraxella sp. The strain of Using DBT as substrate, the biodegradability of the 8 strains was evaluated by HPLC. It was found that 6 strains could degrade HWBHT2 benzothiopheneum. The DBT degradation rate of HWBHT2 bacteria was 45.8% after 3 days culture, and the DBT degradation rate of DBT was 76. 2% after 7 days culture. XFJ2 and HWBHT2 were used to treat Yunnan bituminous coal with organic sulfur removal. The results showed that the two strains could not degrade organic sulfur in Yunnan bituminous coal. Although the medium and physiological and biochemical conditions were improved and optimized, the content of organic sulfur in coal could not be reduced. The reason is that the organic sulfur in Yunnan bituminous coal may not be a DBT substance. Other components of Yunnan bituminous coal can inhibit the growth and metabolism of bacteria. In this study, six strains of bacteria capable of degrading DBT were isolated, and their ability to degrade DBT was studied, which provided a theoretical and practical basis for the further application of coal to remove organic sulfur. However, the removal of organic sulfur from coal by microorganism remains to be further studied.
【學(xué)位授予單位】：昆明理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：TD849

【參考文獻】

相關(guān)期刊論文 前10條

1 王蘭;;煤的微生物脫硫技術(shù)研究進展[J];安徽農(nóng)業(yè)科學(xué);2007年10期

2 任雁秋,鄭坤燦,張捷宇,龐潤芳,袁旭芳;煤的微生物脫硫的實驗研究[J];包頭鋼鐵學(xué)院學(xué)報;2000年02期

3 章春芳;解慶林;張萍;申泰銘;周海妙;;煤炭生物脫硫技術(shù)研究進展[J];礦業(yè)安全與環(huán)保;2008年04期

4 周志付,魏德洲,王英敏,王中謙,崔振揚;燃煤微生物預(yù)處理浮選脫硫的研究進展[J];工業(yè)安全與環(huán)保;2002年02期

5 葉春松，張敬東;燃煤微生物法前期脫硫研究[J];環(huán)境保護科學(xué);1996年03期

6 朱淑琴,尹萍,張萍;間歇式活性污泥除磷的試驗研究[J];環(huán)境工程;1997年06期

7 李國強;李京浩;馬挺;李珊珊;李紅;梁鳳來;劉如林;;苯并噻吩脫硫菌株的篩選及脫硫活性研究[J];環(huán)境科學(xué);2006年06期

8 宋志偉,陳玉平,張鴻波;我國煤炭脫硫技術(shù)現(xiàn)狀及展望[J];國外金屬礦選礦;2000年01期

9 胡雅琴;微生物脫除煤中硫的技術(shù)進展[J];煤化工;2004年02期

10 王蘭;陳佩寒;李敏霞;;煤的微生物脫硫技術(shù)[J];煤氣與熱力;2006年07期

相關(guān)碩士學(xué)位論文 前1條

1 彭秘;云南硯山紅舍克地區(qū)硅酸鹽細(xì)菌的分離及脫硅研究[D];昆明理工大學(xué);2014年

，

本文編號：1976320