【摘要】：基因提取、蛋白質(zhì)結(jié)晶以及新藥研制等工程中需要對種類繁多的生物試劑進(jìn)行高通量、高精度定量分配，傳統(tǒng)的手工作業(yè)勞動強(qiáng)度大、精度低、重復(fù)性差。應(yīng)運(yùn)而生的單通道自動加樣系統(tǒng)在一定程度上克服了手工加樣的缺點(diǎn)，但仍滿足不了越來越繁重的試樣操作要求。多通道自動加樣系統(tǒng)因具有4個(gè)、8個(gè)或者16個(gè)通道，針間距變化可與96孔微孔板一致，一次操作可實(shí)現(xiàn)多個(gè)試樣的分配，不但減少了實(shí)驗(yàn)操作人員的分配操作次數(shù)，而且可提高分配的精密度，是微量試樣高通量處理的發(fā)展趨勢。 本文研究的四通道加樣機(jī)構(gòu)其處理速度為16孔/秒、加樣針的位置精度為±0.2mm、針距之間可實(shí)現(xiàn)9mm與18mm間距變換。懸臂是加樣機(jī)構(gòu)極其重要的組成部分，負(fù)擔(dān)著多個(gè)通道組成的質(zhì)量較大的移動體，移動體在懸臂上做1m/s的高速運(yùn)動，產(chǎn)生的激勵(lì)會導(dǎo)致懸臂振動，進(jìn)而影響處理速度與位置精度，因此要求懸臂的剛度與機(jī)械阻抗越大越好，其振動越小越好。 本文在研究國內(nèi)外現(xiàn)有多通道加樣機(jī)構(gòu)與閱讀大量相關(guān)文獻(xiàn)基礎(chǔ)上，，首先，通過構(gòu)型綜合確定了采用單輸入螺旋式等距分度機(jī)構(gòu)作為加樣針間距調(diào)整機(jī)構(gòu)，并通過工程應(yīng)用分析確定了懸臂式的支撐方式，在此基礎(chǔ)上完成了關(guān)節(jié)結(jié)構(gòu)方案的設(shè)計(jì)。 然后，應(yīng)用模態(tài)分析理論將懸臂簡化為歐拉伯努利梁，從機(jī)械剛度、機(jī)械振動、機(jī)械阻抗三個(gè)方面對懸臂的機(jī)械性能進(jìn)行分析，通過建立系統(tǒng)的數(shù)學(xué)模型，得到影響懸臂剛度的若干因素、計(jì)算出了組合梁的固有頻率、框架的固有頻率；推導(dǎo)出了移動質(zhì)量激勵(lì)下懸臂梁的位移、速度、加速度響應(yīng)與懸臂的機(jī)械阻抗表達(dá)式。著重分析了對懸臂動態(tài)特性影響最大的框架結(jié)構(gòu)，應(yīng)用懲罰函數(shù)法以框架剛度慣量比最大為優(yōu)化目標(biāo)函數(shù)對框架截面尺寸進(jìn)行了優(yōu)化。優(yōu)化后計(jì)算結(jié)果表明，在剛度慣量比增大的情況下，框架的固有頻率提高了24%，靜剛度提高了89%。 最后，搭建了實(shí)驗(yàn)平臺，采用模態(tài)實(shí)驗(yàn)分析方法，應(yīng)用壓電陶瓷傳感器等，通過對壓電陶瓷變形輸出變化的電壓信號進(jìn)行圖像與數(shù)據(jù)采集并運(yùn)用周期法完成框架與組合梁的固有頻率與靜剛度測定后，分析了測量值與理論值產(chǎn)生誤差的原因。
[Abstract]:Gene extraction, protein crystallization and the development of new drugs need to carry out high-throughput and high-precision quantitative distribution of a wide variety of biological reagents. The traditional manual work is characterized by high labor intensity, low precision and poor repeatability. The single channel automatic sampling system has overcome the shortcoming of manual sampling to some extent, but it still can not meet the more and more heavy sample operation requirements. Because the multi-channel automatic sampling system has 4, 8 or 16 channels, the change of needle spacing can be consistent with that of 96 hole microporous plates, and the distribution of multiple samples can be realized in one operation, which not only reduces the number of assignment operations of the laboratory operators, Moreover, the precision of distribution can be improved, which is the development trend of high throughput treatment of trace samples. The four-channel sampling mechanism studied in this paper has the processing speed of 16 holes per second, the accuracy of 鹵0.2 mm in the position of the needle, and the distance between 9mm and 18mm can be transformed between the needle pitch. The cantilever is an extremely important part of the sample adding mechanism. It bears a large mass moving body composed of multiple channels. The high speed motion of the 1m/s on the cantilever will result in the vibration of the cantilever. Therefore, the greater the stiffness and mechanical impedance of the cantilever is, the better the vibration is. Based on the study of the existing multi-channel sampling mechanism at home and abroad and the reading of a large number of related documents, firstly, the single-input spiral isometric indexing mechanism is adopted as the adjusting mechanism of the needle spacing through the configuration synthesis. The cantilever support mode is determined through engineering application analysis, and the design of joint structure scheme is completed on this basis. Then, using modal analysis theory, the cantilever is simplified to Euler Bernoulli beam. The mechanical properties of the cantilever are analyzed from three aspects of mechanical stiffness, mechanical vibration and mechanical impedance, and the mathematical model of the system is established. Some factors affecting cantilever stiffness are obtained, and the natural frequency of composite beam and frame are calculated. The expressions of displacement, velocity, acceleration response and mechanical impedance of cantilever under moving mass excitation are derived. The frame structure which has the greatest influence on the cantilever dynamic characteristics is emphatically analyzed. The frame cross-section size is optimized by using the penalty function method with the maximum stiffness / inertia ratio of the frame as the optimization objective function. The results show that the natural frequency of the frame is increased by 24 and the static stiffness of the frame is increased by 89 when the ratio of stiffness and inertia is increased. Finally, the experiment platform is built, the modal experiment analysis method is adopted, the piezoelectric ceramic sensor and so on are used. By collecting the image and data of the voltage signal of the piezoelectric ceramic deformation output and using the periodic method to measure the natural frequency and static stiffness of the frame and composite beam, the causes of the errors between the measured and theoretical values are analyzed.
【學(xué)位授予單位】：哈爾濱工業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：TH112

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 曾磊;查春光;鄭山鎖;;基于混合懲罰函數(shù)法的型鋼混凝土框架柱優(yōu)化設(shè)計(jì)[J];四川建筑科學(xué)研究;2010年02期

2 李金泉;段冰蕾;李忠明;;新型碼垛機(jī)器人工作空間及影響系數(shù)分析[J];北京郵電大學(xué)學(xué)報(bào);2011年06期

3 修淑麗;李燕;吳碩;陳宋新;郭新;劉彥;;陽性拖帶現(xiàn)象產(chǎn)生的原因和解決方法[J];中國輸血雜志;2006年03期

4 李振權(quán);唐先意;;Matlab優(yōu)化設(shè)計(jì)在機(jī)械設(shè)計(jì)課程設(shè)計(jì)教學(xué)中的應(yīng)用探討[J];成才之路;2010年29期

5 晏紅;趙美云;;電阻應(yīng)變計(jì)在機(jī)械振動測量中的應(yīng)用[J];傳感器世界;2008年08期

6 胡允祥;;機(jī)械阻抗法與鍵圖間的相似關(guān)系及其在頻率響應(yīng)分析中的應(yīng)用[J];紡織基礎(chǔ)科學(xué)學(xué)報(bào);1990年04期

7 彭獻(xiàn);劉子建;洪家旺;;勻變速移動質(zhì)量與簡支梁耦合系統(tǒng)的振動分析[J];工程力學(xué);2006年06期

8 徐陽;盧毓江;秦劍生;陳輝;;測定懸臂梁固有頻率[J];硅谷;2011年17期

9 劉鶴松,崔勝民;基于MATLAB的汽車變速器優(yōu)化設(shè)計(jì)方法[J];哈爾濱工業(yè)大學(xué)學(xué)報(bào);2004年01期

10 周劍平;基于MATLAB的機(jī)械優(yōu)化設(shè)計(jì)[J];黃石理工學(xué)院學(xué)報(bào);2005年03期

本文編號：2427342