【摘要】：銅銹環(huán)棱螺作為一種新興的潛在水生態(tài)毒理學(xué)模式生物,本論文首先在人為控制條件下,對(duì)其室內(nèi)試驗(yàn)條件進(jìn)行了優(yōu)化與篩選,結(jié)果表明:當(dāng)投喂冰鮮小球藻、密度6個(gè)/L水體積、水深10-15cm、泥土底質(zhì)并且水中鈣離子濃度30mg·L-1時(shí),銅銹環(huán)棱螺的體重增長(zhǎng)最為明顯。麻醉效果表明,MgCL2·2H2O對(duì)成螺的麻醉時(shí)間短并且傷害小,可作為實(shí)驗(yàn)用麻醉劑首選。本論文隨后選取了8個(gè)內(nèi)參基因,分析了經(jīng)為期10天暴露于3種不同雌激素下,銅銹環(huán)棱螺內(nèi)臟組織內(nèi)參基因的表達(dá)水平,利用RefFinder在線(xiàn)分析了各個(gè)內(nèi)參基因在銅銹環(huán)棱螺不同雌激素暴露下體內(nèi)表達(dá)水平的穩(wěn)定性,結(jié)果表明,糖體蛋白L7(RPL7)的表達(dá)最為穩(wěn)定。因此,RPL7可作為銅銹環(huán)棱螺在不同外源環(huán)境條件刺激下,定量目標(biāo)基因表達(dá)水平的合適內(nèi)參基因之一。最后,針對(duì)太湖流域銅銹環(huán)棱螺的性比進(jìn)行了初步調(diào)查,發(fā)現(xiàn)其畸變率較高,左右觸角或右側(cè)觸角呈分叉狀或蹼狀的畸變發(fā)生率約為2.7%,對(duì)畸變及正常成螺進(jìn)行了初步的組織學(xué)研究,發(fā)現(xiàn)肌肉組織含有大量寄生蟲(chóng)胚胎,正常組織中,腹足肌肉為平滑肌,精巢內(nèi)含大量成熟簇狀精子,屬進(jìn)化型的II型精子。以上研究結(jié)果皆為發(fā)展以銅銹環(huán)棱螺為指示生物開(kāi)展的水生態(tài)環(huán)境毒理學(xué)研究奠定了基礎(chǔ)。
[Abstract]:As a new potential aquatic ecotoxicological model organism, the laboratory test conditions were optimized and screened under the condition of artificial control. The results showed that when Chlorella vulgaris was fed with chlorella, density 6 / L water volume, water depth 10-15 cm, soil sediment and calcium concentration in water 30mg 路L-1, the body weight of Pterus cerevisiae increased the most obviously. The results showed that when Chlorella vulgaris was fed with chlorella vulgaris, the water depth was 10-15 cm, the soil sediment and the concentration of Ca ~ (2 +) in water were 10 ~ (- 15) cm. The anesthetic effect showed that MgCL2 路2H2O had short anesthetic time and little injury to adult snail, so it could be used as the first choice for experimental anaesthesia. In this paper, eight internal reference genes were selected to analyze the expression level of internal reference genes in visceral tissues of three different estrogens exposed to three different estrogens for 10 days. RefFinder was used to analyze the stability of the expression levels of each internal reference genes in different estrogens exposed to different estrogens. The results showed that the expression of glycosome protein L7 (RPL7) was the most stable. Therefore, RPL7 can be used as one of the suitable internal reference genes for quantitative target gene expression under different external environmental conditions. Finally, the sex ratio of copper rust snail in Taihu Lake Basin was investigated. It was found that the distortion rate was high, and the incidence of forked or webbed distortion in left and right tentacles was about 2.7%. A preliminary histologic study on distortion and normal snail was carried out. It was found that muscle tissue contained a large number of parasite embryos. In normal tissue, gastropoda muscle was smooth muscle and sperm nest contained a large number of mature cluster sperm. Evolutionary type II sperm. The above results laid a foundation for the development of aquatic ecological environment toxicology research with copper rust ring snail as an indicator organism.
【學(xué)位授予單位】：天津農(nóng)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：X171.5

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 Qinqin Lou;Shan Cao;Wei Xu;Yinfeng Zhang;Zhanfen Qin;Wuji Wei;;Molecular characterization and m RNA expression of ribosomal protein L8 in Rana nigromaculata during development and under exposure to hormones[J];Journal of Environmental Sciences;2014年11期

2 王萌;馬陶武;龍奕;劉珊珊;劉佳;彭巾英;;腐殖酸作用下沉積物中納米氧化銅對(duì)銅銹環(huán)棱螺生態(tài)毒性的影響[J];生態(tài)毒理學(xué)報(bào);2014年04期

3 MAKSUP Sarunyaporn;SUPAIBULWATANA Kanyaratt;SELVARAJ Gopalan;;High-quality reference genes for quantifying the transcriptional responses of Oryza sativa L. (ssp. indica and japonica) to abiotic stress conditions[J];Chinese Science Bulletin;2013年16期

4 張紀(jì)亮;王占斌;朱文文;孫平;熊建利;;三苯基錫對(duì)鯽稚魚(yú)肌肉組織學(xué)的影響[J];水生態(tài)學(xué)雜志;2012年05期

5 曾毅;鐘文玨;祝凌燕;劉楠楠;;太湖地區(qū)全沉積物毒性識(shí)別評(píng)估研究[J];中國(guó)科學(xué):化學(xué);2012年08期

6 劉佳;彭巾英;馬陶武;周科;朱程;;沉積物中2,2',4,4'-四溴聯(lián)苯醚(BDE-47)在銅銹環(huán)棱螺體內(nèi)的毒代動(dòng)力學(xué)及其繁殖毒性[J];生態(tài)毒理學(xué)報(bào);2012年03期

7 周科;馬陶武;朱程;劉佳;彭巾英;吳波;;2,2′,4,4′-四溴聯(lián)苯醚(BDE-47)污染沉積物對(duì)銅銹環(huán)棱螺肝胰臟的SOD、CAT和EROD活性的影響[J];環(huán)境科學(xué)學(xué)報(bào);2010年08期

8 李劍;馬梅;王子健;;環(huán)境內(nèi)分泌干擾物的作用機(jī)理及其生物檢測(cè)方法[J];環(huán)境監(jiān)控與預(yù)警;2010年03期

9 ;Laboratory culture of the freshwater benthic gastropod Bellamya aeruginosa (Reeve) and its utility as a test species for sediment toxicity[J];Journal of Environmental Sciences;2010年02期

10 馬陶武;朱程;周科;劉佳;王子健;;銅銹環(huán)棱螺對(duì)鉛污染沉積物慢性脅迫的繁殖響應(yīng)和氧化應(yīng)激反應(yīng)敏感性[J];生態(tài)學(xué)報(bào);2009年10期

相關(guān)會(huì)議論文 前1條

1 李遠(yuǎn)友;杜永兵;譚燕;鄭一軍;胡長(zhǎng)博;陳偉洲;孫澤偉;;有機(jī)錫和辛基苯酚對(duì)孔雀魚(yú)、藍(lán)子魚(yú)的毒性效應(yīng)[A];持久性有機(jī)污染物論壇2006暨第一屆持久性有機(jī)污染物全國(guó)學(xué)術(shù)研討會(huì)論文集[C];2006年

相關(guān)博士學(xué)位論文 前1條

1 伊雄海;農(nóng)藥類(lèi)環(huán)境激素低劑量暴露對(duì)鯽魚(yú)內(nèi)分泌干擾效應(yīng)及生物標(biāo)志物研究[D];上海交通大學(xué);2008年

相關(guān)碩士學(xué)位論文 前1條

1 沈萬(wàn)峗;雙酚A、壬基酚慢性暴露對(duì)斑馬魚(yú)（Danio rerio）生長(zhǎng)和生殖影響的研究[D];華東師范大學(xué);2007年

，

本文編號(hào)：2504113